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Literature DB >> 341154

Cloning of the silk fibroin gene and its flanking sequences.

Abstract

Thirteen Escherichia coli clones containing the whole or a part of the fibroin gene (16 kilobases long) have been isolated. The starting material was DNA extracted from the posterior silk glands of Bombyx mori. Most clones were obtained from sheared DNA fragments linked by poly(dA)-poly(dT) joints to the plasmid pMB9. One of them includes the 5' end of the fibroin gene with a flanking sequence of 12 kilobases, and another includes the 3' end of the gene with a flanking sequence of about 1 kilobase. One clone was obtained by ligation, to pMB9, of a fragment generated by endodeoxy-ribonuclease EcoRI. This clone has a 21-kilobase insertion that probably includes the entire fibroin gene with flanking sequences at both ends. The cleavage sites for endodeoxyribonucleases EcoRI, HindIII, and BamHI have been established for the cloned sequences.

Entities: Chemical Species

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Year: 1977 PMID： 341154 PMCID： PMC431721 DOI： 10.1073/pnas.74.12.5363

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

1. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

Authors: A Dugaiczyk; H W Boyer; H M Goodman
Journal: J Mol Biol Date: 1975-07-25 Impact factor: 5.469

2. Quantitative measurements of fibroin messenger RNA synthesis in the posterior silk gland of normal and mutant Bombyx mori.

Authors: Y Suzuki; E Suzuki
Journal: J Mol Biol Date: 1974-09-15 Impact factor: 5.469

3. Isolation and identification of the messenger RNA for silk fibroin from Bombyx mori.

Authors: Y Suzuki; D D Brown
Journal: J Mol Biol Date: 1972-02-14 Impact factor: 5.469

4. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors: M W McDonell; M N Simon; F W Studier
Journal: J Mol Biol Date: 1977-02-15 Impact factor: 5.469

Cloning of the silk fibroin gene and its flanking sequences.

1. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

2. Quantitative measurements of fibroin messenger RNA synthesis in the posterior silk gland of normal and mutant Bombyx mori.

3. Isolation and identification of the messenger RNA for silk fibroin from Bombyx mori.

4. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

5. Accentuated expression of silk fibroin genes in vivo and in vitro.

6. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.

7. The length of the fibroin gene in the Bombyx mori genome.

8. Isolation of high-molecular-weight DNA from mammalian cells.

9. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.

10. Nucleotide sequence of the rightward operator of phage lambda.

1. Circular mRNA can direct translation of extremely long repeating-sequence proteins in vivo.

2. Assumed initiation site of fibroin gene transcription.

3. Cloning and sequencing of the gene encoding cytochrome c553 from Desulfovibrio vulgaris Hildenborough.

4. Low temperature causes accumulation of unspliced fibroin mRNA precursor molecules in silkworm larvae.

5. Highly repetitive structure and its organization of the silk fibroin gene.

6. Self-complementary DNA sequences within the BRc gene of Chironomus thummi.

7. Discrete-length repeated sequences in eukaryotic genomes.

8. Fine structural analysis of the chicken pro alpha 2 collagen gene.

9. Structure of the transforming region of human cytomegalovirus AD169.

10. Organization of the genes encoding [Fe] hydrogenase in Desulfovibrio vulgaris subsp. oxamicus Monticello.