| Literature DB >> 34115317 |
Fabiana Fernandes Bressan1, André Furugen Cesar de Andrade2, Naira Caroline Godoy Pieri3, Aline Fernanda de Souza1, Ramon Cesar Botigelli4, Laís Vicari de Figueiredo Pessôa1, Kaiana Recchia5, Lucas Simões Machado6, Mayra Hirakawa Glória1, Raquel Vasconcelos Guimarães de Castro7, Diego Feitosa Leal2, Paulo Fantinato Neto1, Simone Maria Massami Kitamura Martins8, Daniele Dos Santos Martins8.
Abstract
Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells.Entities:
Keywords: PGCs; Porcine; Regenerative medicine; Reproduction; Translational medicine
Mesh：
Year: 2021 PMID： 34115317 DOI： 10.1007/s12015-021-10198-8
Source DB: PubMed Journal: Stem Cell Rev Rep ISSN： 2629-3277 Impact factor: 6.692