Literature DB >> 34115

Modification of the tyrosine hydroxylase assay. Increased enzyme activity in the presence of ascorbic acid.

P Lerner, P Nosé, M M Ames, W Lovenberg.   

Abstract

A modification of the tyrosine hydroxylase assay is described in which ascorbate, rather than 2-mercaptoethanol or dihydropteridine reductase with NADPH, is used as the reductant. Enzyme activity is 3-4 times higher with ascorbate than with the other reducing agents. Low blanks are obtained with the ascorbate system provided that catalase is also included. The tissue distribution and kinetic activation of the enzyme have been studied with the ascorbate assay. The results obtained are consistent with the biological and regulatory properties of the enzyme which have been determined with the other reducing systems.

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Year:  1978        PMID： 34115     DOI： 10.1007/bf00963765

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  19 in total

1.  Phospholipid-induced activation of tyrosine hydroxylase from rat brain striatal synaptosomes.

Authors:  J Raese; R L Patrick; J D Barchas
Journal:  Biochem Pharmacol       Date:  1976-10-15       Impact factor: 5.858

2.  Cellular localization of tyrosine hydroxylase by immunohistochemistry.

Authors:  V M Pickel; T H Joh; P M Field; C G Becker; D J Reis
Journal:  J Histochem Cytochem       Date:  1975-01       Impact factor: 2.479

3.  ATP, cyclic AMP, and magnesium increase the affinity of rat striatal tyrosine hydroxylase for its cofactor.

Authors:  W Lovenberg; E A Bruckwick; I Hanbauer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

4.  Evidence for the lack of direct phosphorylation of bovine caudate tyrosine hydroxylase following activation by exposure to enzymatic phosphorylating conditions.

Authors:  T Lloyd; S Kaufman
Journal:  Biochem Biophys Res Commun       Date:  1975-10-06       Impact factor: 3.575

5.  Regional distribution of tyrosine hydroxylase in rat brain.

Authors:  T J Cicero; L G Sharpe; E Robins; S S Grote
Journal:  J Neurochem       Date:  1972-09       Impact factor: 5.372

6.  Studies on tyrosine hydroxylase from bovine adrenal medulla.

Authors:  B Petrack; F Sheppy; V Fetzer
Journal:  J Biol Chem       Date:  1968-02-25       Impact factor: 5.157

7.  The stimulation of partially purified bovine caudate tyrosine hydroxylase by phosphatidyl-L-serine.

Authors:  T Lloyd; S Kaufman
Journal:  Biochem Biophys Res Commun       Date:  1974-08-19       Impact factor: 3.575

8.  Changes of kinetic constant of striatal tyrosine hydroxylase elicited by neuroleptics that impair the function of dopamine receptors.

Authors:  B Zivkovic; A Guidotti
Journal:  Brain Res       Date:  1974-10-25       Impact factor: 3.252

9.  Effect of thioridazine, clozapine and other antipsychotics on the kinetic state of tyrosine hydroxylase and on the turnover rate of dopamine in striatum and nucleus accumbens.

Authors:  B Zivkovic; A Guidotti; A Revuelta; E Costa
Journal:  J Pharmacol Exp Ther       Date:  1975-07       Impact factor: 4.030

10.  Ultrastructural localization of tyrosine hydroxylase in noradrenergic neurons of brain.

Authors:  V M Pickel; T H Joh; D J Reis
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205
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  6 in total

1.  Proceedings of the British Pharmacological Society. Leeds, 12th-14th July 1989. Abstracts.

Authors: 
Journal:  Br J Pharmacol       Date:  1989-10       Impact factor: 8.739

2.  Peroxynitrite inactivation of tyrosine hydroxylase: mediation by sulfhydryl oxidation, not tyrosine nitration.

Authors:  D M Kuhn; C W Aretha; T J Geddes
Journal:  J Neurosci       Date:  1999-12-01       Impact factor: 6.167

3.  Analytical characterization of a sensitive radioassay for tyrosine hydroxylase activity in rodent striatum.

Authors:  Haseeb Ahmad Khan
Journal:  Neurochem Res       Date:  2004-08       Impact factor: 3.996

4.  Evidence for the involvement of a cyclic AMP-independent protein kinase in the activation of soluble tyrosine hydroxylase from rat striatum.

Authors:  D W Andrews; T A Langan; N Weiner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-04       Impact factor: 11.205

5.  Induction of tyrosine hydroxylase gene expression by a nonneuronal nonpituitary-mediated mechanism in immobilization stress.

Authors:  B Nankova; R Kvetnanský; A McMahon; E Viskupic; B Hiremagalur; G Frankle; K Fukuhara; I J Kopin; E L Sabban
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-21       Impact factor: 11.205

6.  Development of a High-Throughput Lysyl Hydroxylase (LH) Assay and Identification of Small-Molecule Inhibitors against LH2.

Authors:  Ashwini K Devkota; John R Veloria; Hou-Fu Guo; Jonathan M Kurie; Eun Jeong Cho; Kevin N Dalby
Journal:  SLAS Discov       Date:  2018-12-27       Impact factor: 3.341
  6 in total

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