Classification of chronic granulomatous disease on the basis of monoclonal antibody-defined surface cytochrome b deficiency.

Flow cytometric quantitative analysis of cytochrome b on outer surface membrane and of oxidative product formation in polymorphonuclear leukocytes (PMNLs) from patients with chronic granulomatous disease was carried out with the use of monoclonal antibody against cytochrome b of human neutrophils and 2', 7'-dichlorofluorescin diacetate. Cytochrome b was present on the outer surface membrane of PMNLs in normal individuals, and its absence on the outer surface membrane was of value in the diagnosis and classification of chronic granulomatous disease. This study has shown that a major type of chronic granulomatous disease is one of the monoclonal antibody-defined surface membrane diseases.