Sequential Organ Failure Assessment (SOFA) Score-Based Factors Predict Early Mortality in High-Risk Patients with Living Donor Liver Transplant.

BACKGROUND Patients with a Sequential Organ Failure Assessment (SOFA) score >7 on post-transplant day (POD) 7 have been reported to have a higher risk of short-term mortality after living donor liver transplant (LDLT). We sought to identify factors that were associated with early mortality in this high-risk population. MATERIAL AND METHODS A total of 102 patients with a high SOFA score (>7) on POD 7 were enrolled, of which 72 (70.6%) were assigned to the survivor group, and the other 30 (29.4%) patients were assigned to the non-survivor group according to post-transplant 3-month results. Demographics, clinical data, operative parameters, and individual SOFA component scores were collected. Independent risk factors for 3-month mortality were identified by multivariate logistic regression analysis using backward elimination procedures. RESULTS Of 102 high SOFA score patients, the 3-month mortality rate after LDLT in our study was 29.4%. Four independent risk factors were indicative for early death: graft-to-recipient weight ratio (GRWR) <0.8 (hazard ratio [HR]=3.00; 95% CI=1.05-8.09; P=0.041), longer warm ischemia time (HR=37.84; 95% CI=1.63-880.77; P=0.024), high liver component of the SOFA score, and cardiovascular component of the SOFA score (liver component: HR=10.39; 95% CI=1.77-60.89; P=0.009 and cardiovascular component: HR=13.34; 95% CI=2.22-80.12; P=0.005). CONCLUSIONS In conclusion, 3-month mortality among patients with high SOFA score on POD 7 is associated with multiple independent risk factors, including smaller GRWR, longer warm ischemia time, and higher category of liver and cardiovascular component of SOFA score. By recognizing high-risk patients earlier, the LDLT outcomes may be improved by timely intensive therapies.

Background

Liver transplantation (LT) remains the optimal treatment for patients with advanced chronic liver disease, acute decompensated liver failure, and hepatocellular carcinoma [1]. Despite great improvement in surgical techniques and progress in managing allograft and critical care [2,3], early vital organs failure remains a major obstacle while managing transplant patients.

Several studies had searched for possible risk factors affecting early mortality after LT [4,5]. However, most of these studies focused on the entire population rather than a particular group with extremely high risk.

The Sequential Organ Failure Assessment (SOFA) [6] score was initially developed to describe and detect organ failure in sepsis settings and has become one of the most comprehensive tools to predict early outcomes in various intensive care units (ICU) [7,8]. The SOFA score [6] was traditionally calculated by a sum of corresponding scores from 6 components – respiratory, coagulation, cardiovascular, liver, renal, and central nervous system (CNS) – with a 0 to 4 scale, shown in Supplementary Table 1. Our group previously reported that patients with a SOFA [6] score >7 on post-transplant day 7 are considered to have a higher risk of short-term mortality after LT [9]. The 3-month mortality rate in this high-risk population was found to be up to 67.5% [7]. This conclusion has been examined to discriminate between survivors and non-survivors [10]. Nevertheless, some patients with high post-transplant SOFA scores still can recover from a critical condition, and the most important factors remains inconclusive.

In this study we tried to validate the SOFA model for a larger cohort in the last 10 years. We aimed to identify decisive factors associated with mortality in the high-risk population of living donor liver transplantation (LDLT) to improve the overall survival.

Material and Methods

Study Population and Data Collection

A total of 527 consecutive LDLT cases, during the period between January 2010 and March 2019, were retrospectively reviewed. Exclusion criteria were: pediatric patients (<18 years old, n=3), lack of essential SOFA information (n=5), and post-transplant day 7 SOFA score ≤7 (n=417). Subsequently, we enrolled 102 patients with high POD 7 SOFA scores (>7). This study was approved by our Institutional Review Board (No. 202001325B0). All patients fit the standard indication for the necessity of liver transplantation. Associated clinical information, laboratory data, and SOFA scores on POD 7 were documented. Preoperative data included recipient and donor age, sex, body mass index, ABO compatibility between the recipient and the donor, model for end-stage liver disease (MELD) score, Child-Pugh class, primary etiology of liver disease, and relevant medical history. We also collected intraoperative data on ischemia time, total operation time, ascites amount, blood loss, and graft-to -recipient weight ratio (GRWR). The survival period was defined as the time from liver transplant until death due to any causes and was censored on the last follow-up date on which the patient was alive. Our primary outcome was post-transplant 3-month survival, and we further divided all patients into survivor (n=72) and non-survivor groups (n=30).

SOFA Score Calculation and SOFA Components Dichotomy

We simplified these SOFA categories by dichotomizing each organ system score by an optimal cut-off value, which was determined by receiver operating characteristic curve to predict post-transplant 3-month mortality. Each SOFA category was dichotomized by cut-off values: 0 for cardiovascular, 2 for coagulation, 1 for renal, 2 for liver, 2 for respiration, and 0 for CNS.

LDLT Programs and Post-transplant Care

Before getting approval for performing a liver transplant, routine work-up must be completed, including liver and vascular images, echocardiogram, pulmonary function test, a variety of urine and blood tests (to determine blood type, clotting function, biochemical studies, and serology screening), and consultations with the transplant team members (hepatologist, surgeon, psychologist, transplant coordinator, social worker, and other specialists, as dictated by particular needs). In addition, patients need to receive pre-liver transplant HLA compatibility screening and lymphocyte cross-matching, and a goal of anti-blood type isoagglutinin titers equal to or less than 1: 64 is desired while performing ABO-incompatible LDLT. Two different pre-transplant rituximab regimens are given according to the anti-A and -B isoagglutinin titers before transplantation, and extra plasmapheresis or plasma exchange was given for patients who failed to reach the goal before receiving transplantation. The details and results of our pre-transplant preparation and post-transplant immunosuppression were illustrated in a previously published article [11].

We performed LDLT using standard technique, as our previous publications described [12,13], and we do not routinely remove the spleen. Regarding prophylactic antibiotics, our routine program suggests a third-generation cephalosporin prescription for uncomplicated patients with a lower MELD score (<20). For patients with a threshold MELD score greater than 20 or who have a complex medical condition (eg, tense ascites, older recipient age, massive intraoperative blood loss), the combination of carbapenem, vancomycin, and echinocandins is administrated [14].

Regarding post-transplant care, ICU care is indispensable to observe and check the progress of vitals and graft condition. The length of ICU stay varies according to individual’s medical stability, and it usually ranges from 5 to 10 days. An extension of ICU stay is indispensable when a patient has organ failure in one or more organs in the first week, including: neurologic or respiratory failure requiring ventilator dependence, persistent shock status, acute deterioration of kidney function causing fluid/electrolyte imbalance requiring renal replacement therapy, uncontrolled or new bleeding episode, any medical emergency that needs a second operation, or any combination of these factors.

Statistics

The independent t test was used to determine relationships between continuous variables (expressed by mean±standard deviations), while Pearson’s chi-square test was used for categorical variables (numbers and percentages). Variable with a P value <0.100 in univariate logistic regression modeling was required as a prior condition before being entered into multivariate analysis to identify independent risk factors for 3-month mortality after transplant. Survival comparisons were constructed with the Kaplan-Meier method with log-rank test. All P values are 2-sided and a level ≤ 0.05 was considered statistically significant. We performed all analyses by using SPSS Statistics version 24.0 (SPSS, Inc., Chicago, IL, USA).

Results

Characteristics of Enrolled Patients

Table 1 shows recipient and donor characteristics, surgery-related factors, and total SOFA score and corresponding components on POD 7 of 102 high-risk LDLT cases. The mean age was 53.3±9.5 and 33.1±9.2 years for recipients and donors, respectively. Most of the recipients were male (n=73, 71.6%), while the sex ratio of donors was about equal (n=47, 46.1%). The leading etiology was viral hepatitis (hepatitis B virus: n=65, 63.7%; hepatitis C virus: n=30, 29.4%), and 22 (21.6%) patients had hepatocellular carcinoma. There were 39 (38.2%) patients with a history of alcohol abuse. The mean MELD score was 24.9±10.5 (median: 24, range: 8–40). Most of our liver grafts were right livers, with a mean GRWR of 0.95±0.24 (median: 0.92, range: 0.54–1.74). The mean cold ischemia time, warm ischemia time, and total procedure time were 45.8±44.6, 37.1±9.6, and 653.5±111 minutes, respectively. Concerning the SOFA score on POD 7, a mean total score of all 102 included patients was 9.8±1.9 (median: 9, range: 8–16). The distribution of case numbers was concentrated at SOFA scores 8 and 9 (n=58/102, 56.9%) among enrolled patients (Figure 1). Of this entire cohort, without regard to high or low SOFA score on POD 7 (n=519), the post-transplantation 3-month survival rate was 91.9%. On the contrary, the 3-month survival rate after transplant was 97.1% for patients with low SOFA (≤7, n=417) and 70.6% for high SOFA score (>7, n=102; p<0.001, Figure 2).

Table 1

Demographic characteristics of 102 patients with post-transplant day 7 SOFA score >7.

Factors	Median or number	Mean±SD	Range
General information
Recipient age, years	54.0	53.3±9.5	28.1–68.8
Recipient BMI, kg/m2	24.2	24.6±3.7	18.2–40.0
Recipient sex, MaleDonor age, years	73 (71.6%)31.8	33.1±9.2	18.5–59.7
Donor BMI, kg/m2	22.9	23.1±2.9	17.5–30.8
Donor sex, MaleMELD score	47 (46.1%)24	24.9±10.5	8–40
HBV infection	65 (63.7%)
HCV infection	30 (29.4%)
Alcohol use	39 (38.2%)
HCC	22 (21.6%)
Ascites, mL	2825	4110.8±4101.3	0–13 500
GRWR, %	0.92	0.95±0.24	0.54–1.74
Blood loss, mL	2475	2962.6±229.0	250–14 500
Cold ischemia time, minutes	30	45.8±44.6	8–246
Warm ischemia time, minutes	36	37.1±9.6	15–64
Operation time, minutes	634	653.5±111.0	460–1219
ICU stay length, days	19	28.2±20.9	4–90
SOFA on POD 7
Cardiovascular component	0	0.2±0.5	0–3
Coagulation component	3	2.9±0.7	0–4
Respiratory component	2	1.6±1.0	0–4
Renal component	1	1.3±1.2	0–4
Liver component	3	3.1±1.0	0–4
CNS component	0	0.8±1.1	0–4
Total score	9	9.8±1.9	8–16

SOFA – Sequential Organ Failure Assessment Score; SD – standard deviation; BMI – body mass index; MELD – model for end-stage liver disease; HBV – hepatitis B virus; HCV – hepatitis C virus; HCC – hepatocellular carcinoma; GRWR – graft recipient weight ratio; CNS – central nervous system; ICU – Intensive Care Unit.

Figure 1

Distribution of SOFA scores on POD 7 among the enrolled population (n=102) in the current study.

Figure 2

Kaplan-Meier plot of the 3-month survival according to high and low SOFA score on POD 7 with a cut-off value of 7. There was significant survival difference (P<0.001) between the 2 groups.

Comparison Between Survivors and Non-survivors

There were 102 patients with a high total SOFA scores (>7) on POD 7 enrolled, of which 72 (70.6%) patients were classified to the survivor group, and the other 30 (29.4%) patients were in the non-survivor group according to post-transplant 3-month results. A comparison between the 2 groups is summarized in Table 2. The prevalence of liver disease etiology, severity of liver disease, and MELD score did not significantly differ between the 2 groups. However, the proportion of patients with older donor age (>45 years old), smaller GRWR (<0.8%), and longer warm ischemia time (>60 minutes) was significantly higher in the non-survivor group. Compared to patients in the survivor group, non-survivors tended to have a higher total SOFA score on POD 7 (10.8±2.2 vs 9.3±1.5, P<0.001), and a higher category in cardiovascular and liver components was associated with early mortality after transplant (both P<0.05).

Table 2

Baseline demographics and clinical characteristics of high SOFA score patients by 3-month mortality.

Factors	Survivors, n=72	Non-survivors, n=30	P value
General information
Recipient age, year-old (>60)	18 (25.0%)	11 (36.7%)	0.234
Recipient sex (Male)	52 (72.2%)	21 (70.0%)	0.821
Recipient BMI, kg/m2	24.6±3.6	24.6±4.0	0.992
Donor age, year-old (>45)	5 (6.9%)	7 (23.3%)	0.019
Donor gender (Male)	33 (45.8%)	14 (46.7%)	0.939
Donor BMI, kg/m2	22.8±2.9	24.0±3.0	0.073
MELD score	25.6±10.2	23.1±11.2	0.279
HBV infection	49 (68.1%)	16 (53.3%)	0.159
HCV infection	22 (30.6%)	8 (26.7%)	0.694
Alcohol use	29 (40.3%)	10 (33.3%)	0.511
HCC	18 (25.0%)	4 (13.3%)	0.192
Pre-transplant RFA history	3 (4.3%)	0 (0.0%)	0.258
Abdominal operation history	13 (18.6%)	4 (13.8%)	0.566
Child-Pugh class (B/C)	21/46 (29.2/63.9%)	13/15 (43.3/50%)	0.375
Ascites, mL (>3000)	34 (47.2%)	14 (46.7%)	0.959
Graft weight, gm	616.9±130.0	550.7±171.9	0.064
GRWR,% (<0.8)	16 (22.2%)	13 (43.3%)	0.031
Blood loss, mL (>3000)	26 (36.1%)	10 (33.3%)	0.789
Cold ischemia time, minutes (>60)	14 (19.4%)	9 (30.0%)	0.245
Warm ischemia time, minutes (>60)	1 (1.4%)	3 (10.0%)	0.041
OP time, minutes (>700)	16 (22.2%)	11 (36.7%)	0.132
SOFA on POD 7
Total SOFA score	9.3±1.5	10.8±2.2	0.001
Cardiovascular component (>0)	4 (5.6%)	7 (23.3%)	0.008
Coagulation component (>2)	57 (79.2%)	25 (83.3%)	0.629
Respiratory component (>2)	10 (13.9%)	4 (13.3%)	0.941
Renal component (>1)	44 (61.1%)	23 (76.7%)	0.132
Liver component (>2)	45 (62.5%)	27 (90.0%)	0.005
CNS component (>0)	23 (31.9%)	14 (46.7%)	0.159
Events
Infection	21 (29.2%)	15 (50.0%)	0.045
Rejection	5 (6.9%)	7 (23.3%)	0.019
Vascular complication	0 (0.0%)	4 (13.3%)	0.002
Cardiopulmonary-related	2 (2.8%)	2 (6.7%)	0.357
Bleeding	2 (2.8%)	2 (6.7%)	0.357
ICU stay length, days	28.4±23.4	27.5±13.9	0.256
Ventilator dependence*	19 (26.4%)	16 (53.3%)	0.009

SOFA – Sequential Organ Failure Assessment Score; BMI – body mass index; MELD – model for end-stage liver disease; OP – operation; BMI – body mass index; HBV – hepatitis B virus; HCV – hepatitis C virus; HCC – hepatocellular carcinoma; RFA – radiofrequency ablation; GRWR – graft-recipient weight ratio; CNS – central nervous system. The value within parentheses indicates optimal cut-off value to predict 3-month mortality after liver transplantation.

Ventilator dependence was defined as failed weaning within 48 hours after transplant or re-intubation of endotracheal tube within 7 days after extubating.

Complications are also shown in Table 2. Significant differences between the 2 groups were found, and there was a higher proportion of infection (50.0% vs 29.2%, p=0.045), rejection (23.3% v.s 6.9%, p=0.019), and vascular complications (13.3% v.s 0.0%, P=0.002) in the non-survivor group. Furthermore, ventilator-dependence (53.3% vs 26.4%, P=0.009), either failure of weaning within 48 hours after transplant or re-intubation of endotracheal tube within 7 days, seemed to be more frequent in the non-survivor group.

Among the survivors, 2 of 3 patients (n=48/72, 66.7%) had a concentrated SOFA score between 8 and 9 on POD 7 (Figure 3A). The composition of case numbers was dispersed among individual SOFA scores in the non-survivor group (Figure 3B).

Figure 3

Distributions of SOFA score on POD 7 for the survivor group (n=72; A) and the non-survivor group (n=30; B). The case numbers decreased as the SOFA score increased in the survivor group but was dispersed in the non-survivor group.

Independent Risk Factors for 3-Month Mortality in Patients with High POD 7 SOFA Scores

In univariate analysis, we analyzed all available clinical factors, and 5 factors (older donor age, smaller GRWR, longer warm ischemia time, and higher liver and cardiovascular component) were subsequently considered as potential risk factors (P<0.100) associated with 3-month mortality after transplant, as shown in Table 3. These potential risk factors were entered into multivariate analysis, showing that GRWR <0.8 (hazard ratio [HR]=3.00; 95% confidence interval [CI]=1.05–8.09; P=0.041) and higher categorized liver component of SOFA score (HR=10.39; 95% CI=1.77–60.89; P=0.009), cardiovascular component of SOFA score (HR=13.34; 95% CI=2.22–80.12; P=0.005), and longer warm ischemia time (HR=37.84; 95% CI=1.63–880.77; P=0.024) were independent risk factors for 3-month mortality after LDLT. We compared Kaplan-Meier survival curves by numbers of risk factors a patient had (21 patients had 0 risk factors, 52 patients had 1 risk factor, 26 patients had 2 risk factors, and 3 patients had 3 risk factors; none of them had all 4 risk factors), and the group with multiple risk factors had inferior outcomes (P<0.001, Figure 4).

Table 3

Uni-/multivariate analyses of clinical characteristics on the 3-month mortality in high POD 7 SOFA score patients by logistic regression.

	Univariate			Multivariate
	HR	95% CI	P value	HR	95% CI	P value
Donor age (>45-year-old)	4.08	1.18–14.11	0.026
Liver component score (>2)	5.40	1.494–19.513	0.010	10.39	1.77–60.89	0.009
Cardiovascular component (>0)	5.17	1.387–19.297	0.014	13.34	2.22–80.12	0.005
Warm ischemia time (>60 minutes)	7.89	0.786–79.171	0.079	37.84	1.63–880.77	0.024
GRWR (<0.8%)	2.68	1.076–6.657	0.034	3.00	1.05–8.59	0.041

POD – post-transplant day; HR – hazard ratio; CI – confidence interval; SOFA – Sequential Organ Failure Assessment Score; GRWR – graft-to-recipient weight ratio. Only significant results were listed.

Figure 4

Kaplan-Meier plot of post-transplant 3-month survival according to the number of independent risks. The more risks the patients carried, the higher mortality rates they would have.

The Association Between High SOFA Components and Adverse Events

It is relatively common to have a high-risk liver component score (>2); for total serum bilirubin > 6 mg/dL, there were 72 cases among that fulfilled the criterion. After excluding patients who were actually having a fair recovery from pre-transplant hyperbilirubinemia (n=30), the remaining 42 patients experienced a variety of conditions that deteriorated their liver function. Most had various degrees of infection (n=21), and 6 of them were directly associated with biliary complications, and 2 were cytomegalovirus disease-related. Acute rejection occurred in 11, and more than half (n=6) eventually lost the graft. Vascular complications (n=4; 3 insufficient portal inflow and 1 hepatic venous outflow obstruction), cardiopulmonary complications (n=3; 2 acute myocardial infarctions and 1 cardiac arrhythmia), and massive hemorrhagic events (n=3) were also responsible for high liver component score (>2) on POD 7.

On the other hand, there were 11 cases with high-risk cardiovascular component scores (>0), such as hypotension with a mean arterial pressure less than 70 mmHg, and only 1 of them was directly heart-originated because of acute myocardial infarction. The rest of them were mostly due to septic shock (n=8), followed by hemorrhagic shock (n=1), and portal vein thrombosis-related graft and multiple organ failures (n=1).

Causes of Death in the High-risk Population

The causes of death are summarized in Table 4. Of the 30 deaths within 90 days after liver transplantation, 7 (23.3%) deaths were considered to be related to rejection, with the majority owing to a combination of either simultaneous or subsequent infection. A primary critical infection accounted for 15 deaths (50.0%): 8 originated from the respiratory tract, 4 were due to intraabdominal infection, 1 was associated with urinary sepsis, 1 was attributed to central nervous system infection, and the last 1 was due to catheter-associated blood stream infection. Other less common causes of death were vascular complications, either portal inflow insufficiency or hepatic venous outflow obstruction (n=4, 13.3%), cardiopulmonary failure (n=2, 6.7%), and bleeding (n=2, 6.7%). Table 4 also reveals the relationship between numbers of independent risk factors (0 to 3) that individuals had and the causes of death. Although rejection and vascular complications seemed to have more risks, there was no strong correlation among SOFA scores, causes of death, and the number of independent risks.

Table 4

Causes of death among non-survivors according to numbers of independent risks contained.

	Non-survivors, n=30 (100.0%)
	Risk no.
Causes	0 risk	1 risk	2 risks	3 risks	Total
Infection	1 (3.3%)	8 (26.7%)	5 (16.7%)	1 (3.3%)	15 (50.0%)
Rejection	0 (0.0%)	1 (6.7%)	4 (13.3%)	2 (6.7%)	7 (23.3%)
Vascular complication*	0 (0.0%)	0 (0.0%)	4 (13.3%)	0 (0.0%)	4 (13.3%)
Cardiopulmonary-related**	0 (0.0%)	1 (3.3%)	1 (3.3%)	0 (0.0%)	2 (6.7%)
Bleeding***	1 (3.3%)	0 (0.0%)	1 (3.3%)	0 (0.0%)	2 (6.7%)
Total	2 (6.7%)	10 (33.3%)	15 (50.0%)	3 (10.0%)	30 (100.0%)
SOFA score on POD 7
SOFA score, mean±SD	10.0±2.8	11.5±2.6	10.6±2.1	10.0±1.0	10.8±2.2
SOFA score, min. to max.	8–12	8–16	8–14	9–11	8–16

POD – post-operative day; SD – standard deviation; min – minimum; max – maximum.

Included 3 insufficient portal inflow and 1 hepatic venous outflow obstruction;

included 1 acute myocardial infarction and 1 pulmonary embolism;

both were massive gastric variceal bleeding.

Discussion

In the current study targeting patients with high POD 7 SOFA score (>7), already considered to be a high-risk population, we found that smaller GRWR, longer warm ischemia time, and higher category of cardiovascular and liver component of SOFA score were correlated to post-transplant short-term adverse outcomes. Interestingly, 2 factors (GRWR and warm ischemia time) outweighed some SOFA components on POD 7 except for cardiovascular and liver component of SOFA score. They can be accessed beforehand to make medical adjustment and prepare in advance for those who might be at very high risk of short-term mortality. In liver allograft recipients, it is important to predict their outcomes. The SOFA scoring scale is an objective assessment to evaluate vital system function, especially in critically ill patients [7]. A convention of GRWR <0.8% has been generally considered as a poor indicator for post-transplant liver function, and it can compromise LT outcomes [15,16]. It is evident that the length of warm ischemia time should be controlled to within 60 minutes to reduce post-transplant complications, especially acute kidney injury [17].

Several SOFA-based prediction models [9,10,18] displayed high discrimination power in predicting mortality after LT, but none paid particular attention to SOFA components and their impact or focused on these sicker patients. We believe that the essence of the SOFA score after LT is the liver component, which is also an indispensable criterion for early allograft dysfunction diagnosis [19]. Moreover, most of our 3-month mortalities were complicated with an infectious condition, which may play a role in predisposing to liver graft dysfunction or be a result of graft failure. On the other hand, the cardiovascular component of the SOFA score is a clinical indicator of circulatory function and severity of sepsis, and reflects treatment response. It is a common problem in dealing with early post-transplant infection [20,21], especially when trying to reduce the risk of rejection by increasing immunosuppressive therapy. When the inflammatory reaction is compromised by anti-rejection drugs, the microbial invasion is often in the process of spreading when revealed clinically [22]. Therefore, a thorough routine examination of patients to identify obscure infectious sources and give early interventions, such as lower immunosuppressive level and apply broad-spectrum antibiotics, is fundamental.

Elsayed et al demonstrated that the duration of ICU stay is also a significant risk factor related to early mortality after living donor liver transplantation [23]. However, in the current study, the ICU stay length between the 2 groups did not differ significantly. A possible explanation might be the relatively short observation period (90 days) and high-risk patients (SOFA score >7 on POD 7) we assessed. An extension of ICU stay was common in the population we chose (mean ICU stay: 28.2 days in the current study vs 9.5 in Elsayed et al), which had a trend of needing more medical care and frequent observation of vital and graft conditions.

Our study found that patients with multiple risk factors tend to have an inferior prognosis. Therefore, efforts are started in donor selection to avoid grafts with small GRWR and preclude complex biliary anastomoses if possible. Detailed and precise surgical planning and execution are needed to avoid unnecessary risks and long ischemia time. In post-transplant critical care, the first step is to identify the subgroup of patients who are at high risk of developing adverse outcomes in the early post-operative period. Preventing and early detection of acute allograft rejection is a major issue to address, especially in patients with infectious concerns. We believe that the adoption of a consummate LDLT protocol and reducing associated risks will help to improve transplant outcomes.

This study has several limitations. First, the retrospective nature restricted the ability to access a serial measure and to evaluate whether the reversal of organ failure helps to improve patient outcomes. Second, this research was conducted in a single tertiary medical center, and conflicts may arise from possible protocol bias between our facility and other facilities. Larger and prospective studies are required to confirm our findings.

Conclusions

In conclusion, 3-month mortality among patients with high SOFA score on POD 7 is associated with multiple independent risk factors, including smaller GRWR, longer warm ischemia time, and higher category of cardiovascular and liver component of SOFA score. By reducing risk factors in high-risk populations, the LDLT outcomes can make greater progress.

Supplementary Table 1

The sequential organ failure assessment (SOFA) score and its components.

SOFA score	0	1	2	3	4
Respiratory
PaO2/FiO2	>400	>300–≤400	>200–≤300	>100–≤200, use of ventilator	≤100, use of ventilator
Coagulation
Platelets, ×103/mm3	>150	>100–≤150	>50–≤100	>20–≤50	≤20
Liver
Bilirubin, mg/dL	<1.2	≥1.2–<2.0	≥2.0–<6.0	≥6.0–<12.0	≥12
Cardiovascular
Hypotension, or catecholamine doses, μg/kg/min for at least one hour	MAP ≥70 mm Hg	MAP <70 mm Hg	Dopamine ≤5, or use of dobutamine	Dopamine >5, or epinephrine or norepinephrine ≤0.1	Dopamine >15, or epinephrine or norepinephrine >0.1
CNS
Glasgow Coma Score	15	13–14	10–12	6–9	<6
Renal
Creatinine, mg/dL, or urine output	<1.2	≥1.2–<2.0	≥2.0–<3.5	≥3.5–<5.0, or <500 mL/day	≥5.0, or <200 mL/day

SOFA – Sequential Organ Failure Assessment; CNS – central nervous system; FiO2 – fractional inspired oxygen; MAP – mean arterial pressure; PaO2 – arterial oxygen tension.