Xiaoqing Zhang1, Xiangyang Guo1, Yanan Zong1, Chuanya Xu1, Jilian Wang1, Bin Zhang1, Chang Liu1, Yueqing Gong2, Lixiang Xue2, Lulin Ma3, Shudong Zhang3, Yi Li4, Hong Zeng5. 1. Department of Anesthesia, Peking University Third Hospital, Huayuan 49# Rd, Haidian Dist., Beijing, China. 2. Biological Sample Bank, Peking University Third Hospital, Beijing, China. 3. Department of Urology, Peking University Third Hospital, Beijing, China. 4. Department of Anesthesia, Peking University Third Hospital, Huayuan 49# Rd, Haidian Dist., Beijing, China. liyicomeon@yahoo.com. 5. Department of Anesthesia, Peking University Third Hospital, Huayuan 49# Rd, Haidian Dist., Beijing, China. dr_zeng@sina.com.
Abstract
BACKGROUND: Intra-operative cell salvage (IOCS) and leukocyte-depleted filter (LDF) are widely used and effective in saving blood. However, the safety issue concerning reinfusion of IOCS-LDF processed blood to renal cell carcinoma (RCC) patients with inferior vena cava (IVC) thrombus were inconclusive for fear of increased risk of cancer metastases. This study intends to analyze the circulating tumor cell (CTC) eliminating effect of IOCS-LDF in 5 RCC-IVC thrombus patients. METHODS: A novel strategy integrating negative enrichment by immunomagnetic beads and immunostaining-fluorescence in situ hybridization with probes identifying aneuploid of 8 and/or 7 were used to detect CTCs from salvages blood. Blood samples were collected from 4 stages in each patient. RESULTS: Of the 5 RCC patients, the number of CTCs decreased (from 3, 4, 10, 7, 3, respectively, to all zero) after IOCS-LDF treatment. The triploid of chromosome 7 and/or chromosome 8 were most common karyotype for RCC patients with IVC thrombus. Tetraploid of chromosome 8 occurred in only one sample and no polypoid (number of chromosome > 4) were found. CONCLUSION: IOCS-LDF might be a promising way of reducing of allogeneic product transfusion based on current preliminary outcome. More convincing conclusions are to be drawn with enlarged sample size and long-term follow-up for patients prognosis.
BACKGROUND: Intra-operative cell salvage (IOCS) and leukocyte-depleted filter (LDF) are widely used and effective in saving blood. However, the safety issue concerning reinfusion of IOCS-LDF processed blood to renal cell carcinoma (RCC) patients with inferior vena cava (IVC) thrombus were inconclusive for fear of increased risk of cancer metastases. This study intends to analyze the circulating tumor cell (CTC) eliminating effect of IOCS-LDF in 5 RCC-IVC thrombuspatients. METHODS: A novel strategy integrating negative enrichment by immunomagnetic beads and immunostaining-fluorescence in situ hybridization with probes identifying aneuploid of 8 and/or 7 were used to detect CTCs from salvages blood. Blood samples were collected from 4 stages in each patient. RESULTS: Of the 5 RCCpatients, the number of CTCs decreased (from 3, 4, 10, 7, 3, respectively, to all zero) after IOCS-LDF treatment. The triploid of chromosome 7 and/or chromosome 8 were most common karyotype for RCCpatients with IVC thrombus. Tetraploid of chromosome 8 occurred in only one sample and no polypoid (number of chromosome > 4) were found. CONCLUSION:IOCS-LDF might be a promising way of reducing of allogeneic product transfusion based on current preliminary outcome. More convincing conclusions are to be drawn with enlarged sample size and long-term follow-up for patients prognosis.
Authors: Christopher T Aquina; Neil Blumberg; Adan Z Becerra; Francis P Boscoe; Maria J Schymura; Katia Noyes; John R T Monson; Fergal J Fleming Journal: Ann Surg Date: 2017-08 Impact factor: 12.969
Authors: A A Klein; C R Bailey; A J Charlton; E Evans; M Guckian-Fisher; R McCrossan; A F Nimmo; S Payne; K Shreeve; J Smith; F Torella Journal: Anaesthesia Date: 2018-07-10 Impact factor: 6.955
Authors: Jonathan Aning; Jamie Dunn; Mark Daugherty; Robert Mason; Richard Pocock; Biddy Ridler; John Thompson; John S McGrath Journal: BJU Int Date: 2012-07-23 Impact factor: 5.588
Authors: Riccardo Campi; Riccardo Tellini; Francesco Sessa; Andrea Mari; Andrea Cocci; Francesco Greco; Alessandro Crestani; Juan Gomez Rivas; Cristian Fiori; Alberto Lapini; Michele Gallucci; Umberto Capitanio; Morgan Roupret; Ronney Abaza; Marco Carini; Sergio Serni; Vincenzo Ficarra; Francesco Porpiglia; Francesco Esperto; Andrea Minervini Journal: Minerva Urol Nefrol Date: 2019-04-05 Impact factor: 3.720
Authors: Brian J Linder; Igor Frank; John C Cheville; Matthew K Tollefson; R Houston Thompson; Robert F Tarrell; Prabin Thapa; Stephen A Boorjian Journal: Eur Urol Date: 2013-01-11 Impact factor: 20.096
Authors: Kathy Lue; Christopher M Russell; John Fisher; Tony Kurian; Gautum Agarwal; Adam Luchey; Michael Poch; Julio M Pow-Sang; Wade J Sexton; Philippe E Spiess Journal: Clin Genitourin Cancer Date: 2015-09-25 Impact factor: 2.872
Authors: Umberto Capitanio; Karim Bensalah; Axel Bex; Stephen A Boorjian; Freddie Bray; Jonathan Coleman; John L Gore; Maxine Sun; Christopher Wood; Paul Russo Journal: Eur Urol Date: 2018-09-19 Impact factor: 20.096