Literature DB >> 3410634

Further studies on the resolution of human mercapt- and nonmercaptalbumin and on human serum albumin in the elderly by high-performance liquid chromatography.

S Era1, T Hamaguchi, M Sogami, K Kuwata, E Suzuki, K Miura, K Kawai, Y Kitazawa, H Okabe, A Noma.   

Abstract

High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on Asahipak GS-520 showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary one to nonmercaptalbumin (HNA). HPLC analysis of HSA on Asahipak ES-520 N showed three peaks, the principal component corresponding to HMA, the secondary one to HNA having mixed disulfide with cysteine or glutathione and the tertiary one to HNA oxidized higher than mixed disulfide. Two kinds of rapid HPLC for the resolution of HSA into HMA and HNA were developed by the present authors. Using these HPLC, the present authors found a significant decrease in the fraction of HMA in the elderly.

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Year:  1988        PMID： 3410634     DOI： 10.1111/j.1399-3011.1988.tb00900.x

Source DB:  PubMed          Journal:  Int J Pept Protein Res        ISSN： 0367-8377


  18 in total

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4.  A lower level of reduced albumin induces serious cardiovascular incidence among peritoneal dialysis patients.

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