Gabriel U Dix1,2, Garett S Jackson1, Kendra R Todd1,2, Jan W van der Scheer3, Jeremy J Walsh1,4, Kathleen A Martin Ginis1,2,5,6, Jonathan P Little7,8. 1. School of Health & Exercise Sciences, University of British Columbia, Kelowna, Canada. 2. International Collaboration on Repair Discoveries (ICORD), Blusson Spinal Cord Centre (BSSC), University of British Columbia, Vancouver, Canada. 3. The Healthcare Improvement Studies (THIS) Institute, Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom. 4. Kinesiology Department, McMaster University, Hamilton, ON, Canada. 5. Department of Medicine, Division of Physical Medicine & Rehabilitation, University of British Columbia, Vancouver, Canada. 6. Centre for Chronic Disease Prevention and Management, University of British Columbia, Kelowna, BC, Canada. 7. School of Health & Exercise Sciences, University of British Columbia, Kelowna, Canada. jonathan.little@ubc.ca. 8. Centre for Chronic Disease Prevention and Management, University of British Columbia, Kelowna, BC, Canada. jonathan.little@ubc.ca.
Abstract
STUDY DESIGN: Secondary analysis of aggregated case series data. OBJECTIVES: To examine the effects of a high-fat/high-carbohydrate meal on leukocyte populations in adults with a chronic SCI. SETTING: University-based laboratories in British Columbia, Canada. METHODS: Ten individuals (M = 9) with a traumatic SCI (>1-year post-injury; M = 15.5 years; n = 2 sensory complete, n = 7 motor complete) participated in this study. Participants arrived fasted (≥12 h) prior to both the control (quiet sitting, no food/drink) and experimental meal conditions (high-fat/high-carb meal: 880 kcal, 52 g fat, 73 g carbohydrates, 29 g protein). Blood samples were taken in the fasted state and at 120-min post-meal/baseline in both conditions. Immune cell counts were assessed using multi-color flow cytometry. RESULTS: A significant time × condition interaction effect was seen in CD3+, CD4+, and CD8+ T cells as well as CD56+ and CD3+/CD56+ natural killer (NK) cells (p < 0.05). CD14+/CD16+ monocytes and CD19+ B cells approached a significant time × condition interaction (p < 0.07). A main effect of time was observed in CD19+ B cells (p < 0.05). Cell counts for T-lymphocytes and NK cells followed the general trend of an increase in the control condition from baseline to 120-min with no change observed following the experimental meal condition. CONCLUSIONS: Following the HFHC meal, immune cells did not show the same general increase observed following the control condition. Future research is needed to determine if there are any potential consequences of these immune cell responses in immunosuppressed populations and if other factors (e.g., diurnal variation) might influence immune cell response.
STUDY DESIGN: Secondary analysis of aggregated case series data. OBJECTIVES: To examine the effects of a high-fat/high-carbohydrate meal on leukocyte populations in adults with a chronic SCI. SETTING: University-based laboratories in British Columbia, Canada. METHODS: Ten individuals (M = 9) with a traumatic SCI (>1-year post-injury; M = 15.5 years; n = 2 sensory complete, n = 7 motor complete) participated in this study. Participants arrived fasted (≥12 h) prior to both the control (quiet sitting, no food/drink) and experimental meal conditions (high-fat/high-carb meal: 880 kcal, 52 g fat, 73 g carbohydrates, 29 g protein). Blood samples were taken in the fasted state and at 120-min post-meal/baseline in both conditions. Immune cell counts were assessed using multi-color flow cytometry. RESULTS: A significant time × condition interaction effect was seen in CD3+, CD4+, and CD8+ T cells as well as CD56+ and CD3+/CD56+ natural killer (NK) cells (p < 0.05). CD14+/CD16+ monocytes and CD19+ B cells approached a significant time × condition interaction (p < 0.07). A main effect of time was observed in CD19+ B cells (p < 0.05). Cell counts for T-lymphocytes and NK cells followed the general trend of an increase in the control condition from baseline to 120-min with no change observed following the experimental meal condition. CONCLUSIONS: Following the HFHC meal, immune cells did not show the same general increase observed following the control condition. Future research is needed to determine if there are any potential consequences of these immune cell responses in immunosuppressed populations and if other factors (e.g., diurnal variation) might influence immune cell response.
Authors: Kathleen A Martin Ginis; Jan W van der Scheer; Amy E Latimer-Cheung; Andy Barrow; Chris Bourne; Peter Carruthers; Marco Bernardi; David S Ditor; Sonja Gaudet; Sonja de Groot; Keith C Hayes; Audrey L Hicks; Christof A Leicht; Jan Lexell; Steven Macaluso; Patricia J Manns; Christopher B McBride; Vanessa K Noonan; Pierre Pomerleau; James H Rimmer; Robert B Shaw; Brett Smith; Karen M Smith; John D Steeves; Dot Tussler; Christopher R West; Dalton L Wolfe; Victoria L Goosey-Tolfrey Journal: Spinal Cord Date: 2017-10-25 Impact factor: 2.772
Authors: Husam Ghanim; Sanaa Abuaysheh; Ching Ling Sia; Kelly Korzeniewski; Ajay Chaudhuri; Jose Manuel Fernandez-Real; Paresh Dandona Journal: Diabetes Care Date: 2009-09-15 Impact factor: 17.152
Authors: Garett S Jackson; Kendra R Todd; Jan W VAN DER Scheer; Jeremy J Walsh; Gabriel U Dix; Kathleen A Martin Ginis; Jonathan P Little Journal: Int J Exerc Sci Date: 2022-07-01