| Literature DB >> 34102169 |
Md Khirul Islam1, Parvez Syed2, Bert Dhondt3, Kamlesh Gidwani4, Kim Pettersson4, Urpo Lamminmäki4, Janne Leivo4.
Abstract
We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-doped-nanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCa-derived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.Entities:
Keywords: Bladder cancer; Glycosylations; ITGA3; Integrin; UEA-lectin; Urine
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Year: 2021 PMID： 34102169 DOI： 10.1016/j.ab.2021.114283
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365