Georg Leixner1, Astrid Voill-Glaninger1, Elisabeth Bonner2, Anna Kreil3, René Zadnikar1, André Viveiros4. 1. Institute for Laboratory Medicine, Klinik Landstrasse, Vienna Healthcare Group, Juchgasse 25, 1030, Vienna, Austria. 2. Institute for Pathology and Microbiology, Klinik Landstrasse, Vienna Healthcare Group, Juchgasse 25, 1030, Vienna, Austria. 3. Emergency Department, Klinik Landstrasse, Vienna Healthcare Group, Juchgasse 25, 1030, Vienna, Austria. 4. Institute for Laboratory Medicine, Klinik Landstrasse, Vienna Healthcare Group, Juchgasse 25, 1030, Vienna, Austria. Electronic address: andre.viveiros@gesundheitsverbund.at.
Abstract
OBJECTIVES: Quick and inexpensive SARS-CoV-2 screening and frontline testing are of growing demand. The aim of our study was to evaluate the performance of the immunochromatographic AMP rapid antigen test (AMP RAT) in comparison with the gold-standard real-time reverse transcription PCR (rRT-PCR) in a hospital cohort. METHODS: A total of 392 patients, who presented consecutively with COVID-19 symptoms at our emergency department, were included in this retrospective study. Two swabs were collected per patient: a nasopharyngeal for the RAT and a combined naso- and oropharyngeal for the rRT-PCR. A positive rRT-PCR (defined as cycle threshold (Ct) < 40) was found in 94 (24%) patients. RESULTS: In our cohort with a median patient age of 70, overall sensitivity and specificity of the AMP RAT was 69.1% (58.8 - 78.3, 95% CI) and 99.7% (98.1 - 100.0, 95% CI), respectively. In patients with a Ct value < 25 and < 30, higher sensitivities of 100.0% (89.4 - 100.0, 95% CI) and 91.8% (81.9 - 97.3%, 95% CI) could be observed. CONCLUSIONS: The AMP RAT showed a high sensitivity in patients with a Ct value < 25 and < 30 and might be useful for frontline testing, whenever rRT-PCR is not rapidly feasible.
OBJECTIVES: Quick and inexpensive SARS-CoV-2 screening and frontline testing are of growing demand. The aim of our study was to evaluate the performance of the immunochromatographic AMP rapid antigen test (AMPRAT) in comparison with the gold-standard real-time reverse transcription PCR (rRT-PCR) in a hospital cohort. METHODS: A total of 392 patients, who presented consecutively with COVID-19 symptoms at our emergency department, were included in this retrospective study. Two swabs were collected per patient: a nasopharyngeal for the RAT and a combined naso- and oropharyngeal for the rRT-PCR. A positive rRT-PCR (defined as cycle threshold (Ct) < 40) was found in 94 (24%) patients. RESULTS: In our cohort with a median patient age of 70, overall sensitivity and specificity of the AMPRAT was 69.1% (58.8 - 78.3, 95% CI) and 99.7% (98.1 - 100.0, 95% CI), respectively. In patients with a Ct value < 25 and < 30, higher sensitivities of 100.0% (89.4 - 100.0, 95% CI) and 91.8% (81.9 - 97.3%, 95% CI) could be observed. CONCLUSIONS: The AMPRAT showed a high sensitivity in patients with a Ct value < 25 and < 30 and might be useful for frontline testing, whenever rRT-PCR is not rapidly feasible.
Authors: Jacqueline Dinnes; Pawana Sharma; Sarah Berhane; Susanna S van Wyk; Nicholas Nyaaba; Julie Domen; Melissa Taylor; Jane Cunningham; Clare Davenport; Sabine Dittrich; Devy Emperador; Lotty Hooft; Mariska Mg Leeflang; Matthew Df McInnes; René Spijker; Jan Y Verbakel; Yemisi Takwoingi; Sian Taylor-Phillips; Ann Van den Bruel; Jonathan J Deeks Journal: Cochrane Database Syst Rev Date: 2022-07-22
Authors: Anastasia Tapari; Georgia G Braliou; Maria Papaefthimiou; Helen Mavriki; Panagiota I Kontou; Georgios K Nikolopoulos; Pantelis G Bagos Journal: Diagnostics (Basel) Date: 2022-06-04
Authors: Lukas E Brümmer; Stephan Katzenschlager; Sean McGrath; Stephani Schmitz; Mary Gaeddert; Christian Erdmann; Marc Bota; Maurizio Grilli; Jan Larmann; Markus A Weigand; Nira R Pollock; Aurélien Macé; Berra Erkosar; Sergio Carmona; Jilian A Sacks; Stefano Ongarello; Claudia M Denkinger Journal: PLoS Med Date: 2022-05-26 Impact factor: 11.613
Authors: Georg Leixner; Astrid Voill-Glaninger; Isabella Krejci; Julia Gaugeler-Kurzweil; Tanja Kusstatscher; Walter Krugluger; André Viveiros Journal: Access Microbiol Date: 2022-06-01