Literature DB >> 3407911

A one-vial method for routine extraction and quantification of free fatty acids in blood and tissue by HPLC.

E Jüngling1, H Kammermeier.   

Abstract

A one-vial procedure has been developed to quantify free fatty acids in human blood serum and rat heart tissue. To allow routine analysis the system has been constructed to allow simultaneous processing of nine samples (Praechromat). The free fatty acids are extracted with Freon 11 and then derivatized to coumarin esters prior to HPLC. The Freon 11 extraction of the free fatty acids is rapid and complete. Neither hydrolytic degradation of natural fatty acid esters nor oxidative damage of unsaturated fatty acids was observed. Fifteen free fatty acids (FFA) were routinely quantified by isocratic elution with high reproducibility (SD less than 4%) and good recovery (0.1 mM FFA: 98-100%, 0.02 mM: 91-105%). The free fatty acids could be determined in the range from 20 pmol to 20 nmol.

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Year:  1988        PMID： 3407911     DOI： 10.1016/0003-2697(88)90136-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

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2.  Fatty acids and eicosanoids regulate gene expression through direct interactions with peroxisome proliferator-activated receptors alpha and gamma.

Authors:  S A Kliewer; S S Sundseth; S A Jones; P J Brown; G B Wisely; C S Koble; P Devchand; W Wahli; T M Willson; J M Lenhard; J M Lehmann
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3.  Elevated levels of nonesterified fatty acids in the myocardium of alloxan diabetic rats.

Authors:  J Chattopadhyay; E W Thompson; H H Schmid
Journal:  Lipids       Date:  1990-06       Impact factor: 1.880

4.  Omega-3 fatty acids regulate gene expression levels differently in subjects carrying the PPARalpha L162V polymorphism.

Authors:  Iwona Rudkowska; Christophe Garenc; Patrick Couture; Marie-Claude Vohl
Journal:  Genes Nutr       Date:  2009-07-08       Impact factor: 5.523

5.  Novel Role of Soluble Epoxide Hydrolase in Regulating Cholesterol in Mammalian Cells.

Authors:  Ahmed Enayetallah; Li Cao; David F Grant
Journal:  Open Drug Metab J       Date:  2007-01-01

6.  Differences in transcriptional activation by the two allelic (L162V Polymorphic) variants of PPARα after Omega-3 fatty acids treatment.

Authors:  Iwona Rudkowska; Mélanie Verreault; Olivier Barbier; Marie-Claude Vohl
Journal:  PPAR Res       Date:  2009-02-25       Impact factor: 4.964
  6 in total

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