Bioactive potential evaluation and purification of compounds from an endophytic fungus Diaporthe longicolla, a resident of Saraca asoca (Roxb.) Willd.

An endophytic fungus (L3), isolated from the leaf tissues of Saraca asoca was identified as D. longicolla by microscopic and molecular methods. The crude extracts of D. longicolla revealed to harbor seven compounds in GC-MS analysis which was subjected to a thin layer chromatography (TLC) for purification and separation of bioactive ingredients. The partially purified fraction from TLC displayed the presence of 2-tridecene (Z) (RT-14.50), 5-tridecene (E) (RT-16.65) and 2,4-di-tert-butylphenol (RT-13.92) in GC-MS. High-performance liquid chromatography (HPLC) was performed to further purify the constituents which led to the collection of 2,4-di-tert-butyl phenol (RT-2.34) with excellent antioxidant activity and antibacterial activity against methicillin resistance Staphylococcus aureus (MRSA).