Literature DB >> 34076596

Principal component analysis is limited to low-resolution analysis in cryoEM.

Carlos Oscar S Sorzano1, Jose Maria Carazo1.   

Abstract

Principal component analysis (PCA) has been widely proposed to analyze flexibility and heterogeneity in cryo-electron microscopy (cryoEM). In this paper, it is argued that (i) PCA is an excellent technique to describe continuous flexibility at low resolution (but not so much at high resolution) and (ii) PCA components should be analyzed in a concerted manner (and not independently). open access.

Entities:  

Keywords:  3D reconstruction; cryo-electron microscopy; image processing; macromolecular machines; protein structure; single-particle cryoEM; structure determination

Year:  2021        PMID： 34076596     DOI： 10.1107/S2059798321002291

Source DB:  PubMed          Journal:  Acta Crystallogr D Struct Biol        ISSN： 2059-7983            Impact factor:   7.652


  4 in total

Review 1.  Emerging Themes in CryoEM─Single Particle Analysis Image Processing.

Authors:  Jose Luis Vilas; Jose Maria Carazo; Carlos Oscar S Sorzano
Journal:  Chem Rev       Date:  2022-07-04       Impact factor: 72.087

2.  Approximating deformation fields for the analysis of continuous heterogeneity of biological macromolecules by 3D Zernike polynomials.

Authors:  David Herreros; Roy R Lederman; James Krieger; Amaya Jiménez-Moreno; Marta Martínez; David Myška; David Strelak; Jiri Filipovic; Ivet Bahar; Jose Maria Carazo; Carlos Oscar S Sanchez
Journal:  IUCrJ       Date:  2021-10-14       Impact factor: 4.769

3.  The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation.

Authors:  Tiziana Ginex; Clara Marco-Marín; Miłosz Wieczór; Carlos P Mata; James Krieger; Paula Ruiz-Rodriguez; Maria Luisa López-Redondo; Clara Francés-Gómez; Roberto Melero; Carlos Óscar Sánchez-Sorzano; Marta Martínez; Nadine Gougeard; Alicia Forcada-Nadal; Sara Zamora-Caballero; Roberto Gozalbo-Rovira; Carla Sanz-Frasquet; Rocío Arranz; Jeronimo Bravo; Vicente Rubio; Alberto Marina; Ron Geller; Iñaki Comas; Carmen Gil; Mireia Coscolla; Modesto Orozco; José Luis Llácer; Jose-Maria Carazo
Journal:  PLoS Pathog       Date:  2022-07-11       Impact factor: 7.464

4.  Application of transport-based metric for continuous interpolation between cryo-EM density maps.

Authors:  Arthur Ecoffet; Geoffrey Woollard; Artem Kushner; Frédéric Poitevin; Khanh Dao Duc
Journal:  AIMS Math       Date:  2021-10-19
  4 in total

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