Emilia Frydrych-Tomczak1, Tomasz Ratajczak2, Łukasz Kościński3, Agnieszka Ranecka3, Natalia Michalak3, Tadeusz Luciński3, Hieronim Maciejewski1,4, Stefan Jurga5, Mikołaj Lewandowski3,5, Marcin K Chmielewski2. 1. Poznań Science and Technology Park, Adam Mickiewicz University Foundation, Rubież 46, 61-612 Poznań, Poland. 2. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland. 3. Institute of Molecular Physics, Polish Academy of Sciences, M. Smoluchowskiego 17, 60-179 Poznań, Poland. 4. Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland. 5. NanoBioMedical Centre, Adam Mickiewicz University, Wszechnicy Piastowskiej 3, 61-614 Poznań, Poland.
Abstract
The structural characterization of glass slides surface-modified with 3-azidopropyltrimethoxysilane and used for anchoring nucleic acids, resulting in the so-called DNA microarrays, is presented. Depending on the silanization conditions, the slides were found to show different oligonucleotide binding efficiency, thus, an attempt was made to correlate this efficiency with the structural characteristics of the silane layers. Atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and X-ray reflectometry (XRR) measurements provided information on the surface topography, chemical composition and thickness of the silane films, respectively. The surface for which the best oligonucleotides binding efficiency is observed, has been found to consist of a densely-packed silane layer, decorated with a high-number of additional clusters that are believed to host exposed azide groups.
The structural characterization of glass slides surface-modified with 3-azidopropyltrimethoxysilane and used for anchoring nucleic acids, resulting in the so-called DNA microarrays, is presented. Depending on the n class="Chemical">silanization conditions, the slides were found to show different oligonucleotide binding efficiency, thus, an attempt was made to correlate this efficiency with the structural characteristics of the silane layers. Atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and X-ray reflectometry (XRR) measurements provided information on the surface topography, chemical composition and thickness of the silane films, respectively. The surface for which the best oligonucleotides binding efficiency is observed, has been found to consist of a densely-packed silane layer, decorated with a high-number of additional clusters that are believed to host exposed azide groups.
Entities:
Keywords:
DNA microarrays; X-ray photoelectron spectroscopy (XPS); atomic force microscopy (AFM); click chemistry; functional layers; organofunctional silanes; structure; surface modification
Authors: Barbara Uszczyńska; Tomasz Ratajczak; Emilia Frydrych; Hieronim Maciejewski; Marek Figlerowicz; Wojciech T Markiewicz; Marcin K Chmielewski Journal: Lab Chip Date: 2012-02-09 Impact factor: 6.799