| Literature DB >> 22318451 |
Barbara Uszczyńska1, Tomasz Ratajczak, Emilia Frydrych, Hieronim Maciejewski, Marek Figlerowicz, Wojciech T Markiewicz, Marcin K Chmielewski.
Abstract
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.Entities:
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Year: 2012 PMID： 22318451 DOI： 10.1039/c2lc21096g
Source DB: PubMed Journal: Lab Chip ISSN： 1473-0189 Impact factor: 6.799