Elena Olăreț1, Brîndușa Bălănucă1,2, Andra Mihaela Onaș1, Jana Ghițman1, Horia Iovu1,3, Izabela-Cristina Stancu1,4, Andrada Serafim1. 1. Advanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Ghe. Polizu Street, 011061 Bucharest, Romania. 2. Department of Organic Chemistry Costin Nenitescu, University Politehnica of Bucharest, 1-7 Ghe. Polizu Street, 011061 Bucharest, Romania. 3. Academy of Romanian Scientists, 54 Splaiul Independentei, 050094 Bucharest, Romania. 4. Faculty of Medical Engineering, University Politehnica of Bucharest, 1-7 Ghe. Polizu Street, 011061 Bucharest, Romania.
Abstract
Mucin is a glycoprotein with proven potential in the biomaterials field, but its use is still underexploited for such applications. The present work aims to produce a synthesis of methacryloyl mucin single-network (SN) hydrogels and their double-cross-linked-network (DCN) counterparts. Following the synthesis of the mucin methacryloyl derivative, various SN hydrogels are prepared through the photopolymerization of methacrylate bonds, using reaction media with different pH values. The SN hydrogels are converted into DCN systems via supplementary cross-linking in tannic acid aqueous solution. The chemical modification of mucin is described, and the obtained product is characterized; the structural modification of mucin is assessed through FTIR spectroscopy, and the circular dichroism and the isoelectric point of methacryloyl mucin is evaluated. The affinity for aqueous media of both SN and DCN hydrogels is estimated, and the mechanical properties of the systems are assessed, both at macroscale through uniaxial compression and rheology tests and also at microscale through nanoindentation tests.
Mucin is a glycoprotein with proven potential in tn class="Chemical">he biomaterials field, but its use is still underexploited for such applications. The present work aims to produce a synthesis of methacryloyl mucin single-network (SN) hydrogels and their double-cross-linked-network (DCN) counterparts. Following the synthesis of themucinmethacryloyl derivative, various SN hydrogels are prepared through the photopolymerization of methacrylate bonds, using reaction media with different pH values. TheSN hydrogels are converted into DCN systems via supplementary cross-linking in tannic acid aqueous solution. The chemical modification of mucin is described, and the obtained product is characterized; the structural modification of mucin is assessed through FTIR spectroscopy, and the circular dichroism and the isoelectric point of methacryloyl mucin is evaluated. The affinity for aqueous media of both SN and DCN hydrogels is estimated, and the mechanical properties of the systems are assessed, both at macroscale through uniaxial compression and rheology tests and also at microscale through nanoindentation tests.
Authors: Jan Busk Madsen; Javier Sotres; Kirsi I Pakkanen; Petr Efler; Birte Svensson; Maher Abou Hachem; Thomas Arnebrant; Seunghwan Lee Journal: Langmuir Date: 2016-09-13 Impact factor: 3.882