Azza A Attia1, Heba S Ramdan2, Rasha A Al-Eisa3, Bassant O A Adle Fadle2, Nahla S El-Shenawy4. 1. Zoology Department, Faculty of Science, Alexandria University, Alexandria 21511, Egypt. 2. Medical Research Institute, Biophysics Department, Alexandria University, Alexandria 21511, Egypt. 3. Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia. 4. Zoology Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt.
Abstract
BACKGROUND: Nanotechnology application has widespread use in many products. Copper nanoparticles (CuNPs) are widely used in industrial applications. The present study was conducted to investigate the effect of the ethanolic saffron extract (ESE) as a natural antioxidant on the hepatotoxicity induced by CuNPs in male mice. METHODS: The characterization of CuNPs was determined using ultraviolet-visible absorption spectroscopy, particle size analysis, zeta potential, Fourier-transform infrared spectroscopy, and electron microscope. The effect of saffron on the hepatotoxicity induced by CuNPs in mice was evaluated by evaluating the survival rate of the mice, oxidative stress, antioxidant capacity, DNA evaluation, as well as its effect on the histology and transmission electron microscope of the liver. RESULTS: The results revealed that all parameters were affected in a dose-dependent manner by CuNPs. These effects have been improved when the treatment of CuNPs is combined with ethanolic saffron extract. CONCLUSIONS: We can conclude that saffron and its bioactive crocin portion can prevent CuNP-induced oxidative liver damage. This substance should be useful as a new pharmacological tool for oxidative stress prevention.
BACKGROUND: Nanotechnology application has widespread use in many products. Copper nanoparticles (CuNPs) are widely used in industrial applications. The present study was conducted to investigate the effect of the ethanolic saffron extract (ESE) as a natural antioxidant on the hepatotoxicity induced by CuNPs in male mice. METHODS: The characterization of CuNPs was determined using ultraviolet-visible absorption spectroscopy, particle size analysis, zeta potential, Fourier-transform infrared spectroscopy, and electron microscope. The effect of saffron on the hepatotoxicity induced by CuNPs in mice was evaluated by evaluating the survival rate of the mice, oxidative stress, antioxidant capacity, DNA evaluation, as well as its effect on the histology and transmission electron microscope of the liver. RESULTS: The results revealed that all parameters were affected in a dose-dependent manner by CuNPs. These effects have been improved when the treatment of CuNPs is combined with ethanolic saffron extract. CONCLUSIONS: We can conclude that saffron and its bioactive crocin portion can prevent CuNP-induced oxidative liver damage. This substance should be useful as a new pharmacological tool for oxidative stress prevention.
Authors: Oscar R Colegio; Christina Van Itallie; Christoph Rahner; James Melvin Anderson Journal: Am J Physiol Cell Physiol Date: 2003-04-16 Impact factor: 4.249
Authors: Ning Li; Constantinos Sioutas; Arthur Cho; Debra Schmitz; Chandan Misra; Joan Sempf; Meiying Wang; Terry Oberley; John Froines; Andre Nel Journal: Environ Health Perspect Date: 2003-04 Impact factor: 9.031