| Literature DB >> 3405203 |
F Yamao1, S Iwagami, Y Azumi, A Muto, S Osawa, N Fujita, A Ishihama.
Abstract
Mycoplasma capricolum uses two tryptophan codons, the "universal" nonsense codon UGA and the universal codon UGG. The bacterium contains two tryptophan tRNAs, one with anticodon UCA, (U: 2'-O-methyl U derivative), and the other with CCA (5'-C: partially 2'-O-methylated). tRNAUCA would translate codons UGA and probably UGG by wobbling. tRNACCA is much less charged by tryptophan in the cells than tRNAUCA, and the intracellular amount of tRNACCA is 5-10 times lower than that of tRNAUCA. The genes for these two tRNAs are separated by a terminator-like structure in a single operon. In vitro transcription experiments suggest that the predominance of tRNAUCA over tRNACCA results from the attenuation of transcription by this terminator-like structure.Entities:
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Year: 1988 PMID： 3405203 DOI： 10.1007/bf00334708
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925