Literature DB >> 34050470

Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.

Mitsuyasu Kawaguchi1, Hidehiko Nakagawa2.   

Abstract

Sirtuins (SIRTs) are a family of NAD+-dependent histone deacetylases (HDACs). In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases, cancers, and even aging. Therefore, the detection of SIRT activity in living cells or tissues would be helpful for diagnosis of a wide range of SIRT-associated diseases. Here, we present methodology to measure SIRT activity in living cells by using our newly developed SIRT fluorescence probe, KST-F-DA.

Entities:  

Keywords:  Fluorescence microscopy; Fluorescence probe; Live imaging; SIRT1; Sirtuin

Year:  2021        PMID： 34050470     DOI： 10.1007/978-1-0716-1258-3_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

Review 1.  Sirtuins in aging and age-related disease.

Authors:  Valter D Longo; Brian K Kennedy
Journal:  Cell       Date:  2006-07-28       Impact factor: 41.582

Review 2.  The biochemistry of sirtuins.

Authors:  Anthony A Sauve; Cynthia Wolberger; Vern L Schramm; Jef D Boeke
Journal:  Annu Rev Biochem       Date:  2006       Impact factor: 23.643

Review 3.  A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.

Authors:  Elizabeth A Specht; Esther Braselmann; Amy E Palmer
Journal:  Annu Rev Physiol       Date:  2016-11-16       Impact factor: 19.318

Review 4.  Sirtuins as regulators of metabolism and healthspan.

Authors:  Riekelt H Houtkooper; Eija Pirinen; Johan Auwerx
Journal:  Nat Rev Mol Cell Biol       Date:  2012-03-07       Impact factor: 94.444

5.  Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.

Authors:  S Imai; C M Armstrong; M Kaeberlein; L Guarente
Journal:  Nature       Date:  2000-02-17       Impact factor: 49.962

Review 6.  Mammalian sirtuins--emerging roles in physiology, aging, and calorie restriction.

Authors:  Marcia C Haigis; Leonard P Guarente
Journal:  Genes Dev       Date:  2006-11-01       Impact factor: 11.361

7.  The histone deacetylase SIRT6 is a tumor suppressor that controls cancer metabolism.

Authors:  Carlos Sebastián; Bernadette M M Zwaans; Dafne M Silberman; Melissa Gymrek; Alon Goren; Lei Zhong; Oren Ram; Jessica Truelove; Alexander R Guimaraes; Debra Toiber; Claudia Cosentino; Joel K Greenson; Alasdair I MacDonald; Liane McGlynn; Fraser Maxwell; Joanne Edwards; Sofia Giacosa; Ernesto Guccione; Ralph Weissleder; Bradley E Bernstein; Aviv Regev; Paul G Shiels; David B Lombard; Raul Mostoslavsky
Journal:  Cell       Date:  2012-12-07       Impact factor: 41.582

Review 8.  Recent progress in the biology and physiology of sirtuins.

Authors:  Toren Finkel; Chu-Xia Deng; Raul Mostoslavsky
Journal:  Nature       Date:  2009-07-30       Impact factor: 49.962

9.  The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.

Authors:  Lei Zhong; Agustina D'Urso; Debra Toiber; Carlos Sebastian; Ryan E Henry; Douangsone D Vadysirisack; Alexander Guimaraes; Brett Marinelli; Jakob D Wikstrom; Tomer Nir; Clary B Clish; Bhavapriya Vaitheesvaran; Othon Iliopoulos; Irwin Kurland; Yuval Dor; Ralph Weissleder; Orian S Shirihai; Leif W Ellisen; Joaquin M Espinosa; Raul Mostoslavsky
Journal:  Cell       Date:  2010-01-22       Impact factor: 41.582

Review 10.  SIRT1, is it a tumor promoter or tumor suppressor?

Authors:  Chu-Xia Deng
Journal:  Int J Biol Sci       Date:  2009-01-21       Impact factor: 6.580
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