Literature DB >> 3404582

DNA-binding activity of hepatitis B e antigen polypeptide lacking the protaminelike sequence of nucleocapsid protein of human hepatitis B virus.

K Matsuda1, S Satoh, H Ohori.   

Abstract

The characteristics of binding of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) polypeptides to hepatitis B virus (HBV) DNA were analyzed. HBcAg polypeptide from recombinant HBV core particles and HBeAg polypeptide from partially purified serum HBeAg were prepared and verified to have molecular weights of 21,500 (P21.5) and of 17,000 (P17) and 18,000 (P18), respectively, by immunoblot analysis. By reaction of these proteins on a nitrocellulose membrane with cloned 32P-HBV DNA, it was revealed that the HBeAg polypeptide, which lacks the C-terminal 34 amino acids of P21.5, as well as the HBcAg polypeptide, bound to the DNA. The secondary structures of nucleocapsid proteins of HBV, woodchuck hepatitis virus, and ground squirrel hepatitis virus were predicted by the Garnier algorithm. Amino acid sequences which, in addition to those of the C-terminal regions, may contribute to binding were proposed to be the 21-amino-acid residues located at amino acids 100 to 120 of the nucleocapsid proteins of these hepadnaviruses.

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Year:  1988        PMID： 3404582      PMCID： PMC253481     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  36 in total

1.  Identification of two HBeAg subspecificities revealed by chemical treatment and enzymatic digestion of liver-derived HBcAg.

Authors:  A Budkowska; B Kalinowska; A Nowosławski
Journal:  J Immunol       Date:  1979-09       Impact factor: 5.422

2.  Demonstration of hepatitis B e antigen (HBeAg) in association with intact Dane particles.

Authors:  H Ohori; S Onodera; N Ishida
Journal:  J Gen Virol       Date:  1979-05       Impact factor: 3.891

3.  Demonstration of hepatitis B e antigen in the core of Dane particles.

Authors:  K Takahashi; Y Akahane; T Gotanda; T Mishiro; M Imai; Y Miyakawa; M Mayumi
Journal:  J Immunol       Date:  1979-01       Impact factor: 5.422

4.  New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants.

Authors:  L O Magnius; J A Espmark
Journal:  J Immunol       Date:  1972-11       Impact factor: 5.422

5.  Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun.

Authors:  P K Vogt; T J Bos; R F Doolittle
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

6.  Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

Authors:  J Garnier; D J Osguthorpe; B Robson
Journal:  J Mol Biol       Date:  1978-03-25       Impact factor: 5.469

7.  The detection of DNA-binding proteins by protein blotting.

Authors:  B Bowen; J Steinberg; U K Laemmli; H Weintraub
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

8.  Demonstration of hepatitis B e antigen in hepatitis B core particles obtained from the nucleus of hepatocytes infected with hepatitis B virus.

Authors:  H Yoshizawa; Y Itoh; J P Simonetti; T Takahashi; A Machida; Y Miyakawa; M Mayumi
Journal:  J Gen Virol       Date:  1979-03       Impact factor: 3.891

9.  A 49,000-dalton polypeptide bearing all antigenic determinants and full immunogenicity of 22-nm hepatitis B surface antigen particles.

Authors:  S Mishiro; M Imai; K Takahashi; A Machida; T Gotanda; Y Miyakawa; M Mayumi
Journal:  J Immunol       Date:  1980-04       Impact factor: 5.422

10.  Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.

Authors:  F Galibert; E Mandart; F Fitoussi; P Tiollais; P Charnay
Journal:  Nature       Date:  1979-10-25       Impact factor: 49.962
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  11 in total

1.  Interaction between hepatitis B virus core protein and reverse transcriptase.

Authors:  L Lott; B Beames; L Notvall; R E Lanford
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

2.  Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: implications for morphogenesis and organization of encapsidated RNA.

Authors:  A Zlotnick; N Cheng; S J Stahl; J F Conway; A C Steven; P T Wingfield
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-02       Impact factor: 11.205

3.  Hepatitis B virus nucleocapsid assembly: primary structure requirements in the core protein.

Authors:  F Birnbaum; M Nassal
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

4.  A recombinant hepatitis B core antigen polypeptide with the protamine-like domain deleted self-assembles into capsid particles but fails to bind nucleic acids.

Authors:  A Gallina; F Bonelli; L Zentilin; G Rindi; M Muttini; G Milanesi
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

5.  Comparison of three different recombinant hepatitis B virus core particles expressed in Escherichia coli.

Authors:  A Maassen; A Rehfeldt; S Kiessig; A Ladhoff; W E Höhne; H Meisel
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

6.  The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly.

Authors:  M Nassal
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

7.  Detection of an RNase H activity associated with hepadnaviruses.

Authors:  S M Oberhaus; J E Newbold
Journal:  J Virol       Date:  1995-09       Impact factor: 5.103

8.  Capsid assembly and involved function analysis of twelve core protein mutants of duck hepatitis B virus.

Authors:  W Yang; J Guo; Z Ying; S Hua; W Dong; H Chen
Journal:  J Virol       Date:  1994-01       Impact factor: 5.103

9.  Phosphorylation and nuclear localization of the hepatitis B virus core protein: significance of serine in the three repeated SPRRR motifs.

Authors:  W Liao; J H Ou
Journal:  J Virol       Date:  1995-02       Impact factor: 5.103

Review 10.  Phosphorylation of the Arginine-Rich C-Terminal Domains of the Hepatitis B Virus (HBV) Core Protein as a Fine Regulator of the Interaction between HBc and Nucleic Acid.

Authors:  Hugues de Rocquigny; Virgile Rat; Florentin Pastor; Jean Luc Darlix; Christophe Hourioux; Philippe Roingeard
Journal:  Viruses       Date:  2020-07-08       Impact factor: 5.048
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