Yanmei Ge1, Fei Pan2, Rui Bai1, Yuan Mao2, Wenli Ji1, Fenfang Wang2, Huacheng Tong3. 1. Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, 211102, China. 2. Nanjing Kingmed Center for Clinical Laboratory Co., Ltd, Nanjing, 210043, China. 3. Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, 211102, China. gym8902@163.com.
Abstract
BACKGROUND: Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China, and revealed the difference of GBS infection between culture and PCR. METHODS: A total of 16,184 pregnant women at 34 to 37 weeks' gestation aged 16-47 years were recruited from Nanjing Kingmed Center for Clinical Laboratory. Nine thousand twenty-two pregnant women received GBS screening by PCR detection only. Seven thousand one hundred sixty-two pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance. RESULTS: The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. Colonization rate was highest in the "25-29 years" age group. The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin, and levofloxacin was 77.5, 68.3, and 52.2%, respectively. CONCLUSIONS: This study revealed the data on the prevalence of GBS colonization in pregnant women at 34 to 37 weeks' gestation in Jiangsu, East China. It compared the difference of the sensitivity to detect GBS between PCR and culture. PCR was expected to become a quick method in pregnancy women conventional detection of GBS infection.
BACKGROUND:Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China, and revealed the difference of GBS infection between culture and PCR. METHODS: A total of 16,184 pregnant women at 34 to 37 weeks' gestation aged 16-47 years were recruited from Nanjing Kingmed Center for Clinical Laboratory. Nine thousand twenty-two pregnant women received GBS screening by PCR detection only. Seven thousand one hundred sixty-two pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance. RESULTS: The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. Colonization rate was highest in the "25-29 years" age group. The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin, and levofloxacin was 77.5, 68.3, and 52.2%, respectively. CONCLUSIONS: This study revealed the data on the prevalence of GBS colonization in pregnant women at 34 to 37 weeks' gestation in Jiangsu, East China. It compared the difference of the sensitivity to detect GBS between PCR and culture. PCR was expected to become a quick method in pregnancy women conventional detection of GBS infection.
Entities:
Keywords:
Antibiotic resistance; Colonization; Group B streptococcus; Prevalence
Authors: Gaurav Kwatra; Marianne C Cunnington; Elizabeth Merrall; Peter V Adrian; Margaret Ip; Keith P Klugman; Wing Hung Tam; Shabir A Madhi Journal: Lancet Infect Dis Date: 2016-05-25 Impact factor: 25.071
Authors: Martina O Chukwu; Rooyen Tinago Mavenyengwa; Charles M Monyama; John Y Bolukaoto; Sogolo L Lebelo; Motlatji Rb Maloba; Maphoshane Nchabeleng; Sylvester Rogers Moyo Journal: Germs Date: 2015-12-02
Authors: Kathleen M Breeding; Bhavana Ragipani; Kun-Uk David Lee; Martin Malik; Tara M Randis; Adam J Ratner Journal: Sci Rep Date: 2016-12-02 Impact factor: 4.379
Authors: Vu Van Du; Pham Thai Dung; Nguyen Linh Toan; Can Van Mao; Nguyen Thanh Bac; Hoang Van Tong; Ho Anh Son; Nghiem Duc Thuan; Nguyen Thanh Viet Journal: Sci Rep Date: 2021-10-21 Impact factor: 4.379