Yifan Tang1, Tao Xie1, Silin Wu1, Qiaoqiao Yang2, Tengfei Liu1, Chen Li1, Shuang Liu1, Zhiyong Shao1,3, Xiaobiao Zhang4,5,6. 1. Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China. 2. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. 3. State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, China. 4. Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China. xiaobiao_zhang@163.com. 5. Digital Medical Research Center, Fudan University, Shanghai, China. xiaobiao_zhang@163.com. 6. Shanghai Key Laboratory of Medical Image Computing and Computer-Assisted Intervention, Shanghai, China. xiaobiao_zhang@163.com.
Abstract
PURPOSE: Somatotroph adenomas are obviously heterogeneous in clinical characteristics, imaging performance, pathological diagnosis and therapeutic effect. The heterogeneity of the tumors, especially for SG and DG type adenomas, have attracted great interest in identifying the specific pathological markers and therapeutic targets of them. However, previous analyses of the molecular characteristics of the subtypes of somatotroph adenomas were performed at genomic and transcriptome level. The proteomic differences between the two subtypes of somatotroph adenomas are still unknown. METHODS: Tumor samples were surgically removed from 10 sporadic pituitary somatotroph adenoma patients and grouped according to the pathological type. Tandem mass tag (TMT)-based quantitative proteomic analysis was employed to analyze the proteomic differences between SG and DG tumors. RESULTS: In total, 228 differentially expressed proteins were identified between SG adenomas and DG adenomas. They were enriched mainly in extracellular matrix (ECM)-receptor interaction, leukocyte transendothelial migration, arrhythmogenic right ventricular cardiomyopathy and DNA replication pathways. Protein-protein interaction (PPI) network analysis indicated that Cadherin-1 and Catenin beta-1 were the most important key proteins in the differences between SG and DG adenomas. Immunohistochemistry (IHC) confirmed the expression levels of the key proteins. CONCLUSIONS: This study provides large-scale proteome molecular characteristics of distinct granulation subtypes of somatotroph adenomas. Compared with DG adenomas, The differential protein of SG adenomas mostly enrich in invasive and proliferative functions and pathways at the proteomic level. Cadherin-1 and Catenin beta-1 play key roles in the different biological characteristics of the two tumor subtypes.
PURPOSE:Somatotroph adenomas are obviously heterogeneous in clinical characteristics, imaging performance, pathological diagnosis and therapeutic effect. The heterogeneity of the tumors, especially for SG and DG type adenomas, have attracted great interest in identifying the specific pathological markers and therapeutic targets of them. However, previous analyses of the molecular characteristics of the subtypes of somatotroph adenomas were performed at genomic and transcriptome level. The proteomic differences between the two subtypes of somatotroph adenomas are still unknown. METHODS:Tumor samples were surgically removed from 10 sporadic pituitary somatotroph adenomapatients and grouped according to the pathological type. Tandem mass tag (TMT)-based quantitative proteomic analysis was employed to analyze the proteomic differences between SG and DG tumors. RESULTS: In total, 228 differentially expressed proteins were identified between SG adenomas and DG adenomas. They were enriched mainly in extracellular matrix (ECM)-receptor interaction, leukocyte transendothelial migration, arrhythmogenic right ventricular cardiomyopathy and DNA replication pathways. Protein-protein interaction (PPI) network analysis indicated that Cadherin-1 and Catenin beta-1 were the most important key proteins in the differences between SG and DG adenomas. Immunohistochemistry (IHC) confirmed the expression levels of the key proteins. CONCLUSIONS: This study provides large-scale proteome molecular characteristics of distinct granulation subtypes of somatotroph adenomas. Compared with DG adenomas, The differential protein of SG adenomas mostly enrich in invasive and proliferative functions and pathways at the proteomic level. Cadherin-1 and Catenin beta-1 play key roles in the different biological characteristics of the two tumor subtypes.
Entities:
Keywords:
Acromegaly; GH; Molecular network; Pituitary adenoma; Quantitative proteomics; Tandem mass tag (TMT)
Authors: Katja Kiseljak-Vassiliades; Nichole E Carlson; Manuel T Borges; B K Kleinschmidt-DeMasters; Kevin O Lillehei; Janice M Kerr; Margaret E Wierman Journal: Endocrine Date: 2014-08-17 Impact factor: 3.633
Authors: Iulia Potorac; Patrick Petrossians; Adrian F Daly; Orsalia Alexopoulou; Sophie Borot; Mona Sahnoun-Fathallah; Frederic Castinetti; France Devuyst; Marie-Lise Jaffrain-Rea; Claire Briet; Florina Luca; Marion Lapoirie; Flavius Zoicas; Isabelle Simoneau; Alpha M Diallo; Ammar Muhammad; Fahrettin Kelestimur; Elena Nazzari; Rogelio Garcia Centeno; Susan M Webb; Marie-Laure Nunes; Vaclav Hana; Véronique Pascal-Vigneron; Irena Ilovayskaya; Farida Nasybullina; Samia Achir; Diego Ferone; Sebastian J C M M Neggers; Brigitte Delemer; Jean-Michel Petit; Christof Schöfl; Gerald Raverot; Bernard Goichot; Patrice Rodien; Bernard Corvilain; Thierry Brue; Franck Schillo; Luaba Tshibanda; Dominique Maiter; Jean-François Bonneville; Albert Beckers Journal: Endocr Relat Cancer Date: 2016-09-20 Impact factor: 5.678