BACKGROUND: The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acute myocarditis. METHODS: To identify a microRNA specific for myocarditis, we performed microRNA microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells and type 17 helper T (Th17) cells after inducing experimental autoimmune myocarditis or myocardial infarction in mice. We also performed qPCR in samples from coxsackievirus-induced myocarditis in mice. We then identified the human homologue for this microRNA and compared its expression in plasma obtained from patients with acute myocarditis with the expression in various controls. RESULTS: We confirmed that Th17 cells, which are characterized by the production of interleukin-17, are a characteristic feature of myocardial injury in the acute phase of myocarditis. The microRNA mmu-miR-721 was synthesized by Th17 cells and was present in the plasma of mice with acute autoimmune or viral myocarditis but not in those with acute myocardial infarction. The human homologue, designated hsa-miR-Chr8:96, was identified in four independent cohorts of patients with myocarditis. The area under the receiver-operating-characteristic curve for this novel microRNA for distinguishing patients with acute myocarditis from those with myocardial infarction was 0.927 (95% confidence interval, 0.879 to 0.975). The microRNA retained its diagnostic value in models after adjustment for age, sex, ejection fraction, and serum troponin level. CONCLUSIONS: After identifying a novel microRNA in mice and humans with myocarditis, we found that the human homologue (hsa-miR-Chr8:96) could be used to distinguish patients with myocarditis from those with myocardial infarction. (Funded by the Spanish Ministry of Science and Innovation and others.).
BACKGROUND: The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acute myocarditis. METHODS: To identify a microRNA specific for myocarditis, we performed microRNA microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells and type 17 helper T (Th17) cells after inducing experimental autoimmune myocarditis or myocardial infarction in mice. We also performed qPCR in samples from coxsackievirus-induced myocarditis in mice. We then identified the human homologue for this microRNA and compared its expression in plasma obtained from patients with acute myocarditis with the expression in various controls. RESULTS: We confirmed that Th17 cells, which are characterized by the production of interleukin-17, are a characteristic feature of myocardial injury in the acute phase of myocarditis. The microRNA mmu-miR-721 was synthesized by Th17 cells and was present in the plasma of mice with acute autoimmune or viral myocarditis but not in those with acute myocardial infarction. The human homologue, designated hsa-miR-Chr8:96, was identified in four independent cohorts of patients with myocarditis. The area under the receiver-operating-characteristic curve for this novel microRNA for distinguishing patients with acute myocarditis from those with myocardial infarction was 0.927 (95% confidence interval, 0.879 to 0.975). The microRNA retained its diagnostic value in models after adjustment for age, sex, ejection fraction, and serum troponin level. CONCLUSIONS: After identifying a novel microRNA in mice and humans with myocarditis, we found that the human homologue (hsa-miR-Chr8:96) could be used to distinguish patients with myocarditis from those with myocardial infarction. (Funded by the Spanish Ministry of Science and Innovation and others.).
Authors: Kristian Thygesen; Joseph S Alpert; Allan S Jaffe; Bernard R Chaitman; Jeroen J Bax; David A Morrow; Harvey D White Journal: J Am Coll Cardiol Date: 2018-08-25 Impact factor: 24.094
Authors: Maarten F Corsten; Anna Papageorgiou; Wouter Verhesen; Paolo Carai; Morten Lindow; Susanna Obad; Georg Summer; Susan L M Coort; Mark Hazebroek; Rick van Leeuwen; Marion J J Gijbels; Erwin Wijnands; Erik A L Biessen; Menno P J De Winther; Frank R M Stassen; Peter Carmeliet; Sakari Kauppinen; Blanche Schroen; Stephane Heymans Journal: Circ Res Date: 2012-06-19 Impact factor: 17.367
Authors: Jennifer M Myers; Leslie T Cooper; David C Kem; Stavros Stavrakis; Stanley D Kosanke; Ethan M Shevach; DeLisa Fairweather; Julie A Stoner; Carol J Cox; Madeleine W Cunningham Journal: JCI Insight Date: 2016-06-16
Authors: Han Zhu; Francisco X Galdos; Daniel Lee; Sarah Waliany; Yuhsin Vivian Huang; Julia Ryan; Katherine Dang; Joel W Neal; Heather A Wakelee; Sunil A Reddy; Sandy Srinivas; Lih-Ling Lin; Ronald M Witteles; Holden T Maecker; Mark M Davis; Patricia K Nguyen; Sean M Wu Journal: Circulation Date: 2022-06-28 Impact factor: 39.918
Authors: Stephane Heymans; James S Ware; Sanjay K Prasad; Amrit S Lota; Mark R Hazebroek; Pantazis Theotokis; Rebecca Wassall; Sara Salmi; Brian P Halliday; Upasana Tayal; Job Verdonschot; Devendra Meena; Ruth Owen; Antonio de Marvao; Alma Iacob; Momina Yazdani; Daniel J Hammersley; Richard E Jones; Riccardo Wage; Rachel Buchan; Fredrik Vivian; Yakeen Hafouda; Michela Noseda; John Gregson; Tarun Mittal; Joyce Wong; Jan Lukas Robertus; A John Baksi; Vassilios Vassiliou; Ioanna Tzoulaki; Antonis Pantazis; John G F Cleland; Paul J R Barton; Stuart A Cook; Dudley J Pennell; Pablo Garcia-Pavia; Leslie T Cooper Journal: Circulation Date: 2022-09-26 Impact factor: 39.918
Authors: Marta Molinero; Iván D Benítez; Jessica González; Clara Gort-Paniello; Anna Moncusí-Moix; Fátima Rodríguez-Jara; María C García-Hidalgo; Gerard Torres; J J Vengoechea; Silvia Gómez; Ramón Cabo; Jesús Caballero; Jesús F Bermejo-Martin; Adrián Ceccato; Laia Fernández-Barat; Ricard Ferrer; Dario Garcia-Gasulla; Rosario Menéndez; Ana Motos; Oscar Peñuelas; Jordi Riera; Antoni Torres; Ferran Barbé; David de Gonzalo-Calvo Journal: Front Med (Lausanne) Date: 2022-02-03
Authors: Sarah Nasreen Schmidt; Wilfried Reichardt; Beat A Kaufmann; Carolin Wadle; Dominik von Elverfeldt; Peter Stachon; Ingo Hilgendorf; Dennis Wolf; Timo Heidt; Daniel Duerschmied; Karlheinz Peter; Christoph Bode; Constantin von Zur Mühlen; Alexander Maier Journal: Cells Date: 2021-12-04 Impact factor: 6.600