Wei Liu1,2, Jian Zheng1,3, Xiaohu Ren1, Yuxuan Xie4, Dafeng Lin4, Peimao Li4, Yuan Lv3, Maggie Pui Man Hoi2, Yanfang Zhang5, Jianjun Liu6,7. 1. Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of Health Toxicology (2020-2024), Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China. 2. Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen, 518020, China. 3. Key Laboratory of Molecular Epidemiology of Hunan Province, School of Medicine, Hunan Normal University, Changsha, 410081, China. 4. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China. 5. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China. 13923431511@139.com. 6. Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of Health Toxicology (2020-2024), Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China. junii8@126.com. 7. Key Laboratory of Molecular Epidemiology of Hunan Province, School of Medicine, Hunan Normal University, Changsha, 410081, China. junii8@126.com.
Abstract
BACKGROUND: Trichloroethylene (TCE) hypersensitivity syndrome (THS) is a dose-independent and potentially life-threatening disease. In this study, we sought to identify THS-related miRNAs and evaluate its potential clinical value. METHODS: Serum samples of five patients and five matched TCE contacts were used for screening differential miRNAs. Another 34 patients and 34 matched TCE contacts were used for verifying significantly differential miRNAs with SYBR™ Green PCR and MGB PCR. The diagnostic model based on these miRNAs was established via the support vector machine (SVM) algorithm. Correlation between differential miRNAs and liver function was analyzed via the Spearman correlation test. RESULTS: A total of 69 miRNAs was found to be differentially expressed. MiR-21-5p and miR-339-5p were verified to have significant higher expressions in patients. The sensitivity, specificity and accuracy of disease model were 100, 75 and 86%, respectively. The two miRNAs showed significant correlations with liver function. CONCLUSION: These findings suggested that miRNAs profiles in serum of THS patients had changed significantly, and miR-21-5p and miR-339-5p were associated with THS.
BACKGROUND:Trichloroethylene (TCE) hypersensitivity syndrome (THS) is a dose-independent and potentially life-threatening disease. In this study, we sought to identify THS-related miRNAs and evaluate its potential clinical value. METHODS: Serum samples of five patients and five matched TCE contacts were used for screening differential miRNAs. Another 34 patients and 34 matched TCE contacts were used for verifying significantly differential miRNAs with SYBR™ Green PCR and MGB PCR. The diagnostic model based on these miRNAs was established via the support vector machine (SVM) algorithm. Correlation between differential miRNAs and liver function was analyzed via the Spearman correlation test. RESULTS: A total of 69 miRNAs was found to be differentially expressed. MiR-21-5p and miR-339-5p were verified to have significant higher expressions in patients. The sensitivity, specificity and accuracy of disease model were 100, 75 and 86%, respectively. The two miRNAs showed significant correlations with liver function. CONCLUSION: These findings suggested that miRNAs profiles in serum of THSpatients had changed significantly, and miR-21-5p and miR-339-5p were associated with THS.
Authors: Melissa C Friesen; Sarah J Locke; Yu-Cheng Chen; Joseph B Coble; Patricia A Stewart; Bu-Tian Ji; Bryan Bassig; Wei Lu; Shouzheng Xue; Wong-Ho Chow; Qing Lan; Mark P Purdue; Nathaniel Rothman; Roel Vermeulen Journal: Ann Occup Hyg Date: 2014-09-01
Authors: Natalie P Archer; Carrie M Bradford; John F Villanacci; Neil E Crain; Richard L Corsi; David M Chambers; Tonia Burk; Benjamin C Blount Journal: J Environ Sci Health A Tox Hazard Subst Environ Eng Date: 2015-08-11 Impact factor: 2.269
Authors: Weihsueh A Chiu; Jennifer Jinot; Cheryl Siegel Scott; Susan L Makris; Glinda S Cooper; Rebecca C Dzubow; Ambuja S Bale; Marina V Evans; Kathryn Z Guyton; Nagalakshmi Keshava; John C Lipscomb; Stanley Barone; John F Fox; Maureen R Gwinn; John Schaum; Jane C Caldwell Journal: Environ Health Perspect Date: 2012-12-18 Impact factor: 9.031