Literature DB >> 33987023

A Python script to merge Sanger sequences.

Cen Chen1,2,3, Bingguo Lu4, Xiaofang Huang5, Chuyun Bi5, Lili Zhao6, Yunzhuo Hu5, Xuanyang Chen5, Shiqiang Lin5, Kai Huang1.   

Abstract

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
© 2021 Chen et al.

Entities:  

Keywords:  Merge; Python script; Sanger sequencing

Year:  2021        PMID： 33987023      PMCID： PMC8086567          DOI： 10.7717/peerj.11354

Source DB:  PubMed          Journal:  PeerJ        ISSN： 2167-8359            Impact factor:   2.984


  21 in total

1.  An Eulerian path approach to DNA fragment assembly.

Authors:  P A Pevzner; H Tang; M S Waterman
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-14       Impact factor: 11.205

2.  Genome assembly reborn: recent computational challenges.

Authors:  Mihai Pop
Journal:  Brief Bioinform       Date:  2009-05-29       Impact factor: 11.622

3.  Nucleotide sequence of bacteriophage phi X174 DNA.

Authors:  F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  Nature       Date:  1977-02-24       Impact factor: 49.962

4.  A python script to design site-directed mutagenesis primers.

Authors:  Chuyun Bi; Xiaofang Huang; Dan Tang; Yuanyuan Shi; Lixiang Zhou; Yunzhuo Hu; Xuanyang Chen; Shiqian Qi; Shiqiang Lin
Journal:  Protein Sci       Date:  2019-12-27       Impact factor: 6.725

5.  A new algorithm for DNA sequence assembly.

Authors:  R M Idury; M S Waterman
Journal:  J Comput Biol       Date:  1995       Impact factor: 1.479

6.  Genome engineering using the CRISPR-Cas9 system.

Authors:  F Ann Ran; Patrick D Hsu; Jason Wright; Vineeta Agarwala; David A Scott; Feng Zhang
Journal:  Nat Protoc       Date:  2013-10-24       Impact factor: 13.491

7.  Bioconductor: open software development for computational biology and bioinformatics.

Authors:  Robert C Gentleman; Vincent J Carey; Douglas M Bates; Ben Bolstad; Marcel Dettling; Sandrine Dudoit; Byron Ellis; Laurent Gautier; Yongchao Ge; Jeff Gentry; Kurt Hornik; Torsten Hothorn; Wolfgang Huber; Stefano Iacus; Rafael Irizarry; Friedrich Leisch; Cheng Li; Martin Maechler; Anthony J Rossini; Gunther Sawitzki; Colin Smith; Gordon Smyth; Luke Tierney; Jean Y H Yang; Jianhua Zhang
Journal:  Genome Biol       Date:  2004-09-15       Impact factor: 13.583

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Cryo-EM structure of C9ORF72-SMCR8-WDR41 reveals the role as a GAP for Rab8a and Rab11a.

Authors:  Dan Tang; Jingwen Sheng; Liangting Xu; Xiechao Zhan; Jiaming Liu; Hui Jiang; Xiaoling Shu; Xiaoyu Liu; Tizhong Zhang; Lan Jiang; Cuiyan Zhou; Wenqi Li; Wei Cheng; Zhonghan Li; Kunjie Wang; Kefeng Lu; Chuangye Yan; Shiqian Qi
Journal:  Proc Natl Acad Sci U S A       Date:  2020-04-17       Impact factor: 11.205

10.  Fragment merger: an online tool to merge overlapping long sequence fragments.

Authors:  Trevor G Bell; Anna Kramvis
Journal:  Viruses       Date:  2013-03-12       Impact factor: 5.048
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.