| Literature DB >> 33977457 |
Eva G Barreales1, Jesús F Aparicio2.
Abstract
Polyene antibiotics are macrolide antifungal compounds obtained by fermentation of producer Streptomyces strains. Here we describe commonly used methods for polyene production, detection, and their subsequent extraction and purification. While bioassays are used to detect these compounds based on their biological activity, quantification by spectrophotometry or high-performance liquid chromatography (HPLC ) relies on their physiochemical properties and is more reliable.Entities:
Keywords: Antifungal agent; Bioassay; HPLC; Polyene macrolide; Streptomyces; UV-visible spectrum
Year: 2021 PMID： 33977457 DOI： 10.1007/978-1-0716-1358-0_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745