Kelly Dimovska Nilsson1, Anthi Karagianni1, Ibrahim Kaya1,2,3, Marcus Henricsson4, John S Fletcher5. 1. Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30, Gothenburg, Sweden. 2. Department of Psychiatry and Neurochemistry, Sahlgrenska Academy at the University of Gothenburg, 413 45, Mölndal, Sweden. 3. Medical Mass Spectrometry Laboratory, Department of Pharmaceutical Biosciences, Uppsala University, 751 05, Uppsala, Sweden. 4. Department of Molecular and Clinical Medicine/Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, 41345, Gothenburg, Sweden. 5. Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30, Gothenburg, Sweden. john.fletcher@chem.gu.se.
Abstract
This work assesses the potential of new water cluster-based ion beams for improving the capabilities of secondary ion mass spectrometry (SIMS) for in situ lipidomics. The effect of water clusters was compared to carbon dioxide clusters, along with the effect of using pure water clusters compared to mixed water and carbon dioxide clusters. A signal increase was found when using pure water clusters. However, when analyzing cells, a more substantial signal increase was found in positive ion mode when the water clusters also contained carbon dioxide, suggesting that additional reactions are in play. The effects of using a water primary ion beam on a more complex sample were investigated by analyzing brain tissue from an Alzheimer's disease transgenic mouse model. The results indicate that the ToF-SIMS results are approaching those from MALDI as ToF-SIMS was able to image lyso-phosphocholine (LPC) lipids, a lipid class that for a long time has eluded detection during SIMS analyses. Gangliosides, sulfatides, and cholesterol were also imaged.
This work assesses the potential of new pan class="Chemical">water cluster-based ion beams for improving the capan>bilities of secondary ion mass span class="Chemical">pectrometry (SIMS) for in situ lipidomics. The effect of water clusters was compared to carbon dioxide clusters, along with the effect of using pure water clusters compared to mixed water and carbon dioxide clusters. A signal increase was found when using pure water clusters. However, when analyzing cells, a more substantial signal increase was found in positive ion mode when the water clusters also contained carbon dioxide, suggesting that additional reactions are in play. The effects of using a water primary ion beam on a more complex sample were investigated by analyzing brain tissue from an Alzheimer's disease transgenic mouse model. The results indicate that the ToF-SIMS results are approaching those from MALDI as ToF-SIMS was able to image lyso-phosphocholine (LPC) lipids, a lipid class that for a long time has eluded detection during SIMS analyses. Gangliosides, sulfatides, and cholesterol were also imaged.
Entities:
Keywords:
Alzheimer’s; Imaging; Lipids; Mass spectrometry; SIMS; Water clusters
Authors: Daniel Weibel; Steve Wong; Nicholas Lockyer; Paul Blenkinsopp; Rowland Hill; John C Vickerman Journal: Anal Chem Date: 2003-04-01 Impact factor: 6.986