| Literature DB >> 3397368 |
L L Ibric1, W F Benedict, A R Peterson.
Abstract
A reproducible method is described for the separation and quantification of ascorbic acid and dehydroascorbic acid by ion-pairing reverse-phase high performance liquid chromatography and detection by absorbance at 232 nm. Lowest detectable concentrations with a linear response of detection were 5 nmol for ascorbic acid and 50 nmol for dehydroascorbic acid. This method was applied to the analysis of C3H/10T1/2 cells and culture medium after influx or efflux experiments and single or multiple treatments with ascorbic acid. Subsequent measurement of the radioactivity in the eluted fractions increased the detectability of both ascorbic acid and dehydroascorbic acid to 10 to 20 pmol.Entities:
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Year: 1988 PMID： 3397368 DOI： 10.1007/bf02623604
Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364