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Literature DB >> 33954948

Targeted Lipidomics of Drosophila melanogaster During Development.

Abstract

Lipids play critical roles in developmental processes, and alterations in lipid metabolism are linked to a wide range of human diseases, including neurodegeneration, cancer, metabolic diseases, and microbial infections. Drosophila melanogaster, more commonly known as the fruit fly, is a powerful organism for developmental biology and human disease research. We have previously developed a comprehensive biochemical tool, based on liquid chromatography-mass spectrometry (LC-MS), to probe the dynamics of lipid remodeling during D. melanogaster development. This chapter introduces a step-by-step protocol for extracting and analyzing lipids across all developmental stages (embryo, larvae, pupa, and adult) of D. melanogaster. The targeted semi-quantitative approach offers a comprehensive coverage of more than 400 lipid species spanning the lipid classes, glycerophospholipids, sphingolipids, triacylglycerols, and sterols.

Entities: Chemical Disease Species

Keywords: Development; Drosophila melanogaster; Lipid remodeling; Lipidomics; Metabolism; Multiple reaction monitoring

Year: 2021 PMID： 33954948 DOI： 10.1007/978-1-0716-1410-5_13

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

35 in total

1. Survival strategies of a sterol auxotroph.

Authors: Maria Carvalho; Dominik Schwudke; Julio L Sampaio; Wilhelm Palm; Isabelle Riezman; Gautam Dey; Gagan D Gupta; Satyajit Mayor; Howard Riezman; Andrej Shevchenko; Teymuras V Kurzchalia; Suzanne Eaton
Journal: Development Date: 2010-11 Impact factor: 6.868

2. Analysis of Drosophila lipids by matrix-assisted laser desorption/ionization mass spectrometric imaging.

Authors: Ann-Christin Niehoff; Hans Kettling; Alexander Pirkl; Yin Ning Chiang; Klaus Dreisewerd; Joanne Y Yew
Journal: Anal Chem Date: 2014-11-05 Impact factor: 6.986

3. Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids.

Authors: Yan Huang; Sheng Huang; Sin Man Lam; Zhihua Liu; Guanghou Shui; Yong Q Zhang
Journal: J Cell Sci Date: 2016-09-21 Impact factor: 5.285

4. A combined proteomic and genetic analysis identifies a role for the lipid desaturase Desat1 in starvation-induced autophagy in Drosophila.

Authors: Katja Köhler; Erich Brunner; Xue Li Guan; Karin Boucke; Urs F Greber; Sonali Mohanty; Julia M I Barth; Markus R Wenk; Ernst Hafen
Journal: Autophagy Date: 2009-10-22 Impact factor: 16.016

5. Biochemical membrane lipidomics during Drosophila development.

Authors: Xue Li Guan; Gianluca Cestra; Guanghou Shui; Antje Kuhrs; Ralf B Schittenhelm; Ernst Hafen; F Gisou van der Goot; Carmen C Robinett; Maurizio Gatti; Marcos Gonzalez-Gaitan; Markus R Wenk
Journal: Dev Cell Date: 2012-12-20 Impact factor: 12.270

6. A drosophila genetic resource of mutants to study mechanisms underlying human genetic diseases.

Authors: Shinya Yamamoto; Manish Jaiswal; Wu-Lin Charng; Tomasz Gambin; Ender Karaca; Ghayda Mirzaa; Wojciech Wiszniewski; Hector Sandoval; Nele A Haelterman; Bo Xiong; Ke Zhang; Vafa Bayat; Gabriela David; Tongchao Li; Kuchuan Chen; Upasana Gala; Tamar Harel; Davut Pehlivan; Samantha Penney; Lisenka E L M Vissers; Joep de Ligt; Shalini N Jhangiani; Yajing Xie; Stephen H Tsang; Yesim Parman; Merve Sivaci; Esra Battaloglu; Donna Muzny; Ying-Wooi Wan; Zhandong Liu; Alexander T Lin-Moore; Robin D Clark; Cynthia J Curry; Nichole Link; Karen L Schulze; Eric Boerwinkle; William B Dobyns; Rando Allikmets; Richard A Gibbs; Rui Chen; James R Lupski; Michael F Wangler; Hugo J Bellen
Journal: Cell Date: 2014-09-25 Impact factor: 66.850

1. Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and MALDI-TOF/TOF Mass Spectrometry Imaging.

Authors: Huaguo Chen; Lei Peng; Chao Zhao; Zongwei Cai; Xin Zhou
Journal: Foods Date: 2022-05-28

1 in total

Targeted Lipidomics of Drosophila melanogaster During Development.

1. Survival strategies of a sterol auxotroph.

2. Analysis of Drosophila lipids by matrix-assisted laser desorption/ionization mass spectrometric imaging.

3. Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids.

4. A combined proteomic and genetic analysis identifies a role for the lipid desaturase Desat1 in starvation-induced autophagy in Drosophila.

5. Biochemical membrane lipidomics during Drosophila development.

6. A drosophila genetic resource of mutants to study mechanisms underlying human genetic diseases.

Review 7. Wax, sex and the origin of species: Dual roles of insect cuticular hydrocarbons in adaptation and mating.

8. Effects of diet and development on the Drosophila lipidome.

Review 9. Drosophila tools and assays for the study of human diseases.

10. Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.

1. Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and MALDI-TOF/TOF Mass Spectrometry Imaging.