| Literature DB >> 33950506 |
Oliver Pagel1, Laxmikanth Kollipara1, Albert Sickmann2,3,4.
Abstract
In mass spectrometry-based proteomics, relative quantitative approaches enable differential protein abundance analysis. Isobaric labeling strategies, such as tandem mass tags (TMT), provide simultaneous quantification of several samples (e.g., up to 16 using 16plex TMTpro) owing to its multiplexing capability. This technology improves sample throughput and thereby minimizes both measurement time and overall experimental variation. However, TMT-based MS data processing and statistical analysis are probably the crucial parts of this pipeline to obtain reliable, plausible, and significantly quantified results. Here, we provide a step-by-step guide to the analysis and evaluation of TMT quantitative proteomics data.Keywords: Data analysis; Relative quantitative proteomics; TMT
Year: 2021 PMID： 33950506 DOI： 10.1007/978-1-0716-1024-4_28
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745