Literature DB >> 3394934

A bioluminescent assay for the determination of phosphoenolpyruvate carboxykinase activity in nanogram-sized tissue samples.

M Wimmer1.   

Abstract

A highly specific and sensitive assay for the determination of phosphoenolpyruvate carboxykinase (PEPCK) in nanogram-sized tissue samples is described. This test system is based on the stoichiometric transformation of phosphoenolpyruvate into ATP. In a subsequent step ATP is quantified by bioluminescent techniques. The applicability of this assay system is shown by measurements in liver samples with normal and high PEPCK activity levels.

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Year:  1988        PMID： 3394934     DOI： 10.1016/0003-2697(88)90646-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

1.  Changes in the acinar activity patterns of phosphoenolpyruvate carboxykinase in livers of male and female rats upon feeding a high protein and a high fat diet.

Authors:  M Wimmer; C Luttringer; M Colombi
Journal:  Histochemistry       Date:  1990

2.  Effects of starvation and refeeding a high carbohydrate diet on the intra-acinar distribution pattern of phosphoenolpyruvate carboxykinase activity in the liver of male and female rats.

Authors:  M Wimmer
Journal:  Histochemistry       Date:  1989

3.  The effects of acetylsalicylic acid on phosphoenolpyruvate carboxykinase activity and acinar heterotopy in livers from juvenile and adult rats.

Authors:  M Wimmer; C Luttringer; M Colombi
Journal:  Histochemistry       Date:  1990

4.  The development of the acinar heterotopic pattern of phosphoenolpyruvate carboxykinase activity in the newborn rat.

Authors:  M Wimmer; C Luttringer; M Colombi
Journal:  Histochemistry       Date:  1990

5.  Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on tryptophan and glucose homeostasis in the most TCDD-susceptible and the most TCDD-resistant species, guinea pigs and hamsters.

Authors:  M Unkila; M Ruotsalainen; R Pohjanvirta; M Viluksela; E MacDonald; J T Tuomisto; K Rozman; J Tuomisto
Journal:  Arch Toxicol       Date:  1995       Impact factor: 5.153

6.  Enzyme activity patterns of phosphoenolpyruvate carboxykinase, pyruvate kinase, glucose-6-phosphate-dehydrogenase and malic enzyme in human liver.

Authors:  M Wimmer; C Luttringer; M Colombi
Journal:  Histochemistry       Date:  1990

7.  Phosphoenolpyruvate carboxykinase activity patterns in the liver acinus of diabetic and diabetic and estrogen treated rats.

Authors:  M Wimmer
Journal:  Histochemistry       Date:  1989

8.  Sex differences of the influence of T3 on the topical distribution of phosphoenolpyruvate carboxykinase activity in the liver acinus.

Authors:  M Wimmer
Journal:  Histochemistry       Date:  1989

9.  Metabolic adaptation of pigs to a Mycoplasma hyopneumoniae and Lawsonia intracellularis dual challenge.

Authors:  Emma T Helm; Amanda C Outhouse; Kent J Schwartz; Steven M Lonergan; Shelby M Curry; Jack C M Dekkers; Nicholas K Gabler
Journal:  J Anim Sci       Date:  2018-07-28       Impact factor: 3.159

10.  The acinar heterotopy of phosphoenolpyruvate carboxykinase activity in rat liver under the influence of the oestrous cycle.

Authors:  M Wimmer
Journal:  Histochemistry       Date:  1993-08
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