Literature DB >> 33928575

Preparation and Characterization of siRNA-Loaded Liposomes.

Yang Liu1, Leaf Huang2.   

Abstract

The major challenge for RNAi-based therapy is the fabrication of the delivery system that meet the requirement of clinical applicability. Liposome-derived nanoparticles (NPs) are one of the best investigated systems for in vivo siRNA delivery. In the recent years, we have successfully redesigned the conventional cationic liposomes into Liposome/Protamine/hyaluronic acid (LPH) NPs and Lipid-Calcium-Phosphate (LCP) NPs in order to increase the in vivo gene silencing effect and reduce the toxicity. Here we describe the preparation of LPH and LCP NPs loaded with siRNA, and characterization analysis including size distribution, trapping efficiency, and in vivo activity. This protocol could be used for in vivo delivery of siRNA to target genes in cancer cells.

Entities:  

Keywords:  Calcium phosphate; Cationic lipid; In vivo silencing effect; Liposomes; Nanoparticles; Size; Trapping efficiency; siRNA delivery

Year:  2021        PMID： 33928575     DOI： 10.1007/978-1-0716-1298-9_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  In vivo delivery of RNAi with lipid-based nanoparticles.

Authors:  Leaf Huang; Yang Liu
Journal:  Annu Rev Biomed Eng       Date:  2011-08-15       Impact factor: 9.590

Review 2.  Therapeutic potential of retroviral RNAi vectors.

Authors:  Eric Devroe; Pamela A Silver
Journal:  Expert Opin Biol Ther       Date:  2004-03       Impact factor: 4.388

Review 3.  Efficient and targeted delivery of siRNA in vivo.

Authors:  Min Suk Shim; Young Jik Kwon
Journal:  FEBS J       Date:  2010-12       Impact factor: 5.542

4.  In vivo gene transfer via intravenous administration of cationic lipid-protamine-DNA (LPD) complexes.

Authors:  S Li; L Huang
Journal:  Gene Ther       Date:  1997-09       Impact factor: 5.250

5.  Sticky overhangs enhance siRNA-mediated gene silencing.

Authors:  Anne-Laure Bolcato-Bellemin; Marie-Elise Bonnet; Gaëlle Creusat; Patrick Erbacher; Jean-Paul Behr
Journal:  Proc Natl Acad Sci U S A       Date:  2007-10-03       Impact factor: 11.205

6.  An efficient and low immunostimulatory nanoparticle formulation for systemic siRNA delivery to the tumor.

Authors:  Sumio Chono; Shyh-Dar Li; Christine C Conwell; Leaf Huang
Journal:  J Control Release       Date:  2008-07-13       Impact factor: 9.776

7.  Patisiran, an RNAi Therapeutic, for Hereditary Transthyretin Amyloidosis.

Authors:  David Adams; Alejandra Gonzalez-Duarte; William D O'Riordan; Chih-Chao Yang; Mitsuharu Ueda; Arnt V Kristen; Ivailo Tournev; Hartmut H Schmidt; Teresa Coelho; John L Berk; Kon-Ping Lin; Giuseppe Vita; Shahram Attarian; Violaine Planté-Bordeneuve; Michelle M Mezei; Josep M Campistol; Juan Buades; Thomas H Brannagan; Byoung J Kim; Jeeyoung Oh; Yesim Parman; Yoshiki Sekijima; Philip N Hawkins; Scott D Solomon; Michael Polydefkis; Peter J Dyck; Pritesh J Gandhi; Sunita Goyal; Jihong Chen; Andrew L Strahs; Saraswathy V Nochur; Marianne T Sweetser; Pushkal P Garg; Akshay K Vaishnaw; Jared A Gollob; Ole B Suhr
Journal:  N Engl J Med       Date:  2018-07-05       Impact factor: 91.245

8.  Tumor-targeted delivery of siRNA by self-assembled nanoparticles.

Authors:  Shyh-Dar Li; Yun-Ching Chen; Michael J Hackett; Leaf Huang
Journal:  Mol Ther       Date:  2007-10-09       Impact factor: 11.454

9.  Characterization of cationic lipid-protamine-DNA (LPD) complexes for intravenous gene delivery.

Authors:  S Li; M A Rizzo; S Bhattacharya; L Huang
Journal:  Gene Ther       Date:  1998-07       Impact factor: 5.250

Review 10.  Knocking down barriers: advances in siRNA delivery.

Authors:  Kathryn A Whitehead; Robert Langer; Daniel G Anderson
Journal:  Nat Rev Drug Discov       Date:  2009-02       Impact factor: 84.694
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  2 in total

1.  MCP mediated active targeting calcium phosphate hybrid nanoparticles for the treatment of orthotopic drug-resistant colon cancer.

Authors:  Shaobo Bai; Yang Sun; Ying Cheng; Weiliang Ye; Chenchao Jiang; Miao Liu; Qifeng Ji; Bangle Zhang; Qibing Mei; Daozhou Liu; Siyuan Zhou
Journal:  J Nanobiotechnology       Date:  2021-11-17       Impact factor: 10.435

2.  Pharmaceutical Development and Design of Thermosensitive Liposomes Based on the QbD Approach.

Authors:  Dorina Gabriella Dobó; Zsófia Németh; Bence Sipos; Martin Cseh; Edina Pallagi; Dániel Berkesi; Gábor Kozma; Zoltán Kónya; Ildikó Csóka
Journal:  Molecules       Date:  2022-02-24       Impact factor: 4.411
  2 in total

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