Zerrin Barut1, Ayca Turer Cabbar2, Seda Gulec Yilmaz3, Fatma Tuba Akdeniz3, Mustafa Aytek Simsek2, Betul Capar4, Muzaffer Degertekin2, Altay Burak Dalan3, Halit Yerebakan5, Turgay Isbir6. 1. Department of Basic Medical Science, Faculty of Dentistry, Antalya Bilim University, Antalya, Turkey. 2. Department of Cardiology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey. 3. Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey. 4. Department of Molecular Medicine, Institute of Health Sciences, Yeditepe University, Istanbul, Turkey. 5. Department of Cardiovascular Surgery, Faculty of Medicine, Yeditepe University, Istanbul, Turkey. 6. Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey; turgay.isbir@yeditepe.edu.tr.
Abstract
BACKGROUND/AIM: Left ventricular hypertrophy (LVH) involves increased muscular mass of the left ventricle due to increased cardiomyocyte size and is caused by cardiomyopathies. Several microRNAs (miRNAs) have been implicated in processes that contribute to heart disease. This study aimed to examine miRNA-133, miRNA-26 and miRNA-378 as candidate biomarkers to define prognosis in patients with LVH. PATIENTS AND METHODS: The study group consisted of 70 patients who were diagnosed with LVH and 16 unaffected individuals who served as the control group. Real-time polymerase chain reaction (RT-PCR) was used to analyze serum miRNA-133, miRNA-26, and miRNA-378 expression levels in LVH patients and the control group. Receiver operating characteristic (ROC) curve analysis was performed to assess the diagnostic capability of miRNA-378. RESULTS: When crossing threshold (CT) values were compared between patient and control samples, we found that there were no statistically significant differences in miRNA-133 and miRNA-26 CT values, while the miRNA-378 expression was significantly increased in LVH patients. ROC analysis demonstrated that the expression levels of miRNA-378 (AUC=0.484, p=0.0013) were significantly different between groups. CONCLUSION: We observed a statistically significant relationship between miRNA-378 expression levels and LVH, suggesting that circulating miRNA-378 may be used as a novel biomarker to distinguish patients who have LVH from those who do not. Copyright
BACKGROUND/AIM: Left ventricular hypertrophy (LVH) involves increased muscular mass of the left ventricle due to increased cardiomyocyte size and is caused by cardiomyopathies. Several microRNAs (miRNAs) have been implicated in processes that contribute to heart disease. This study aimed to examine miRNA-133, miRNA-26 and miRNA-378 as candidate biomarkers to define prognosis in patients with LVH. PATIENTS AND METHODS: The study group consisted of 70 patients who were diagnosed with LVH and 16 unaffected individuals who served as the control group. Real-time polymerase chain reaction (RT-PCR) was used to analyze serum miRNA-133, miRNA-26, and miRNA-378 expression levels in LVH patients and the control group. Receiver operating characteristic (ROC) curve analysis was performed to assess the diagnostic capability of miRNA-378. RESULTS: When crossing threshold (CT) values were compared between patient and control samples, we found that there were no statistically significant differences in miRNA-133 and miRNA-26 CT values, while the miRNA-378 expression was significantly increased in LVH patients. ROC analysis demonstrated that the expression levels of miRNA-378 (AUC=0.484, p=0.0013) were significantly different between groups. CONCLUSION: We observed a statistically significant relationship between miRNA-378 expression levels and LVH, suggesting that circulating miRNA-378 may be used as a novel biomarker to distinguish patients who have LVH from those who do not. Copyright
Authors: L Thierfelder; H Watkins; C MacRae; R Lamas; W McKenna; H P Vosberg; J G Seidman; C E Seidman Journal: Cell Date: 1994-06-03 Impact factor: 41.582
Authors: Scot J Matkovich; Wei Wang; Yizheng Tu; William H Eschenbacher; Lisa E Dorn; Gianluigi Condorelli; Abhinav Diwan; Jeanne M Nerbonne; Gerald W Dorn Journal: Circ Res Date: 2009-11-05 Impact factor: 17.367