Literature DB >> 33908000

Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass Spectrometry.

Elsa Wagner-Rousset1, Olivier Colas1, Stéphane Chenu1, Yannis-Nicolas François2, Davy Guillarme3,4, Sarah Cianferani5, Yury O Tsybin6, Jonathan Sjögren7, Arnaud Delobel8, Alain Beck9.   

Abstract

Middle-up LC-MS antibody characterization workflows using reduction or IdeS digestion for a focused assessment of N-glycan profiling of three representative glycoengineered monoclonal antibodies (mAbs), namely, obinutuzumab (GlycomAb technology, Glycart/Roche), benralizumab (Potelligent Technology, BioWa, Kyowa Kirin) and mAb B (kifunensine) and compared to mAb A, produced in a common CHO cell line. In addition, EndoS or EndoS2 enzyme are used for quantitative determination of Fc-glycan core afucosylation and high mannose for these antibodies, as requested by health authorities for Fc-competent therapeutics mAbs critical quality attributes (CQAs).

Entities:  

Keywords:  ADCC; Benralizumab; EndoS; EndoS2; High mannose; IdeS; Kifunensine; Low fucose; Mass spectrometry; Obinutuzumab

Year:  2021        PMID： 33908000     DOI： 10.1007/978-1-0716-1241-5_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  25 in total

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Authors:  Yu-Tzu Tai; Patrick A Mayes; Chirag Acharya; Mike Y Zhong; Michele Cea; Antonia Cagnetta; Jenny Craigen; John Yates; Louise Gliddon; William Fieles; Bao Hoang; James Tunstead; Amanda L Christie; Andrew L Kung; Paul Richardson; Nikhil C Munshi; Kenneth C Anderson
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Review 2.  Cutting-edge multi-level analytical and structural characterization of antibody-drug conjugates: present and future.

Authors:  Alain Beck; Valentina D'Atri; Anthony Ehkirch; Szabolcs Fekete; Oscar Hernandez-Alba; Rabah Gahoual; Emmanuel Leize-Wagner; Yannis François; Davy Guillarme; Sarah Cianférani
Journal:  Expert Rev Proteomics       Date:  2019-02-18       Impact factor: 3.940

Review 3.  Next generation antibody drugs: pursuit of the 'high-hanging fruit'.

Authors:  Paul J Carter; Greg A Lazar
Journal:  Nat Rev Drug Discov       Date:  2017-12-01       Impact factor: 84.694

4.  Development of Comprehensive Online Two-Dimensional Liquid Chromatography/Mass Spectrometry Using Hydrophilic Interaction and Reversed-Phase Separations for Rapid and Deep Profiling of Therapeutic Antibodies.

Authors:  Dwight R Stoll; David C Harmes; Gregory O Staples; Oscar G Potter; Carston T Dammann; Davy Guillarme; Alain Beck
Journal:  Anal Chem       Date:  2018-04-13       Impact factor: 6.986

Review 5.  Glycosylation of biosimilars: Recent advances in analytical characterization and clinical implications.

Authors:  Bastiaan L Duivelshof; Wim Jiskoot; Alain Beck; Jean-Luc Veuthey; Davy Guillarme; Valentina D'Atri
Journal:  Anal Chim Acta       Date:  2019-08-22       Impact factor: 6.558

6.  Increasing the efficacy of CD20 antibody therapy through the engineering of a new type II anti-CD20 antibody with enhanced direct and immune effector cell-mediated B-cell cytotoxicity.

Authors:  Ekkehard Mössner; Peter Brünker; Samuel Moser; Ursula Püntener; Carla Schmidt; Sylvia Herter; Roger Grau; Christian Gerdes; Adam Nopora; Erwin van Puijenbroek; Claudia Ferrara; Peter Sondermann; Christiane Jäger; Pamela Strein; Georg Fertig; Thomas Friess; Christine Schüll; Sabine Bauer; Joseph Dal Porto; Christopher Del Nagro; Karim Dabbagh; Martin J S Dyer; Sibrand Poppema; Christian Klein; Pablo Umaña
Journal:  Blood       Date:  2010-03-01       Impact factor: 22.113

7.  Marketing approval of mogamulizumab: a triumph for glyco-engineering.

Authors:  Alain Beck; Janice M Reichert
Journal:  MAbs       Date:  2012-07-01       Impact factor: 5.857

8.  Structural Analysis of Monoclonal Antibodies by Ultrahigh Resolution MALDI In-Source Decay FT-ICR Mass Spectrometry.

Authors:  Yuri E M van der Burgt; David P A Kilgour; Yury O Tsybin; Kristina Srzentić; Luca Fornelli; Alain Beck; Manfred Wuhrer; Simone Nicolardi
Journal:  Anal Chem       Date:  2019-01-08       Impact factor: 6.986

9.  NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.

Authors:  Maria Lorna A De Leoz; David L Duewer; Adam Fung; Lily Liu; Hoi Kei Yau; Oscar Potter; Gregory O Staples; Kenichiro Furuki; Ruth Frenkel; Yunli Hu; Zoran Sosic; Peiqing Zhang; Friedrich Altmann; Clemens Grunwald-Grube; Chun Shao; Joseph Zaia; Waltraud Evers; Stuart Pengelley; Detlev Suckau; Anja Wiechmann; Anja Resemann; Wolfgang Jabs; Alain Beck; John W Froehlich; Chuncui Huang; Yan Li; Yaming Liu; Shiwei Sun; Yaojun Wang; Youngsuk Seo; Hyun Joo An; Niels-Christian Reichardt; Juan Echevarria Ruiz; Stephanie Archer-Hartmann; Parastoo Azadi; Len Bell; Zsuzsanna Lakos; Yanming An; John F Cipollo; Maja Pucic-Bakovic; Jerko Štambuk; Gordan Lauc; Xu Li; Peng George Wang; Andreas Bock; René Hennig; Erdmann Rapp; Marybeth Creskey; Terry D Cyr; Miyako Nakano; Taiki Sugiyama; Pui-King Amy Leung; Paweł Link-Lenczowski; Jolanta Jaworek; Shuang Yang; Hui Zhang; Tim Kelly; Song Klapoetke; Rui Cao; Jin Young Kim; Hyun Kyoung Lee; Ju Yeon Lee; Jong Shin Yoo; Sa-Rang Kim; Soo-Kyung Suh; Noortje de Haan; David Falck; Guinevere S M Lageveen-Kammeijer; Manfred Wuhrer; Robert J Emery; Radoslaw P Kozak; Li Phing Liew; Louise Royle; Paulina A Urbanowicz; Nicolle H Packer; Xiaomin Song; Arun Everest-Dass; Erika Lattová; Samanta Cajic; Kathirvel Alagesan; Daniel Kolarich; Toyin Kasali; Viv Lindo; Yuetian Chen; Kudrat Goswami; Brian Gau; Ravi Amunugama; Richard Jones; Corné J M Stroop; Koichi Kato; Hirokazu Yagi; Sachiko Kondo; C T Yuen; Akira Harazono; Xiaofeng Shi; Paula E Magnelli; Brian T Kasper; Lara Mahal; David J Harvey; Roisin O'Flaherty; Pauline M Rudd; Radka Saldova; Elizabeth S Hecht; David C Muddiman; Jichao Kang; Prachi Bhoskar; Daniele Menard; Andrew Saati; Christine Merle; Steven Mast; Sam Tep; Jennie Truong; Takashi Nishikaze; Sadanori Sekiya; Aaron Shafer; Sohei Funaoka; Masaaki Toyoda; Peter de Vreugd; Cassie Caron; Pralima Pradhan; Niclas Chiang Tan; Yehia Mechref; Sachin Patil; Jeffrey S Rohrer; Ranjan Chakrabarti; Disha Dadke; Mohammedazam Lahori; Chunxia Zou; Christopher Cairo; Béla Reiz; Randy M Whittal; Carlito B Lebrilla; Lauren Wu; Andras Guttman; Marton Szigeti; Benjamin G Kremkow; Kelvin H Lee; Carina Sihlbom; Barbara Adamczyk; Chunsheng Jin; Niclas G Karlsson; Jessica Örnros; Göran Larson; Jonas Nilsson; Bernd Meyer; Alena Wiegandt; Emy Komatsu; Helene Perreault; Edward D Bodnar; Nassur Said; Yannis-Nicolas Francois; Emmanuelle Leize-Wagner; Sandra Maier; Anne Zeck; Albert J R Heck; Yang Yang; Rob Haselberg; Ying Qing Yu; William Alley; Joseph W Leone; Hua Yuan; Stephen E Stein
Journal:  Mol Cell Proteomics       Date:  2019-10-07       Impact factor: 5.911

10.  CD16 pre-ligation by defucosylated tumor-targeting mAb sensitizes human NK cells to γc cytokine stimulation via PI3K/mTOR axis.

Authors:  Cristina Capuano; Chiara Pighi; Roberta Maggio; Simone Battella; Stefania Morrone; Gabriella Palmieri; Angela Santoni; Christian Klein; Ricciarda Galandrini
Journal:  Cancer Immunol Immunother       Date:  2020-01-16       Impact factor: 6.968
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