Characterization of Peripheral Anterior Synechiae Formation After Microhook Ab-interno Trabeculotomy Using a 360-Degree Gonio-Camera.

PURPOSE: To investigate the prevalence, locations, and characteristics of peripheral anterior synechiae (PAS) formation after microhook ab-interno trabeculotomy (µLOT), a minimally invasive glaucoma surgery, using a 360-degree gonio-camera, gonioscope GS-1 (NIDEK Co., Gamagori, Japan). SUBJECTS AND METHODS: A total of 105 consecutive eyes of 75 subjects with open-angle glaucoma were analyzed. The eyes had undergone µLOT or combined µLOT and cataract surgery as an initial glaucoma surgery. Postoperative PAS formation was evaluated in 16 iridocorneal angle images with the best focus covering 360 degrees in each eye.
RESULTS: Compared to baseline, at 225±226 days postoperatively, the intraocular pressure and number of antiglaucoma medications decreased significantly (p<0.01, respectively). PAS developed in 86% of eyes. The mean number of iridocorneal angle images that showed PAS in all eyes was 4.1 (26%) in the total circumference, 3.1 (39%) within the µLOT incision, and 1.0 (13%) outside of the µLOT incision; the rate was significantly (p<0.0001) higher within the incision than outside of the incision. Moreover, the higher total PAS rate and that within the incision may be associated with later postoperative days (p=0.01 and 0.004, respectively), that outside of the incision with µLOT alone rather than the combined surgery, and with shallower preoperative central anterior chamber depth (p=0.048 and 0.04, respectively), calculated by the mixed-effect model.
CONCLUSION: The current results showed the characteristics of PAS formation after µLOT using 360-degree gonio-images, and the formation rate was significantly higher within the µLOT incision. The PAS within and outside of the incision had different causes.

Introduction

Glaucoma is the leading cause of irreversible blindness worldwide, and the global number of affected individuals is expected to increase with the growing elderly population.1,2 Because elevated intraocular pressure (IOP) is a major risk factor for glaucoma development and progression,3 it is necessary for IOP to be controlled with medications, laser treatment, and surgery. Ab-externo filtration surgery such as trabeculectomy is remarkably effective and considered the gold standard; however, it may lead to significant complications, the consideration of which can sometimes delay glaucoma surgery until all other less invasive treatment options are attempted. Various minimally invasive glaucoma surgery (MIGS) procedures are safer and less traumatic with an ab-interno approach for patients with mild-to-moderate glaucoma or those who are refractory to standard medical therapy.4 Therefore, MIGS could decrease the number of patients who require more invasive surgeries to control the IOP. These surgeries also lowered the hurdles for surgical intervention to provide better IOP control and reduce the glaucoma medication burden. Ab-interno trabeculotomy is a MIGS that increases trabecular outflow4–8 and is increasing in popularity. The surgical mechanism is assumed to be elimination of aqueous flow resistance by cleavage of the trabecular meshwork (TM) and inner walls of Schlemm’s canal at the point of outflow resistance.9,10 However, peripheral anterior synechiae (PAS) may develop after ab-externo trabeculotomy (modified 360-degree suture trabeculotomy) that obstruct the TM, decrease the outflow, and possibly weaken the IOP-lowering effect.11,12 However, PAS formation after trabeculotomy has not been studied in detail.

Microhook ab-interno trabeculotomy (µLOT) is a novel ab-interno trabeculotomy-related glaucoma surgery in which Tanito ab–interno Trabeculotomy Microhooks (Inami & Co., Ltd., Tokyo, Japan) are used to incise the TM; several studies have reported the effectiveness of µLOT for decreasing both the IOP and number of antiglaucoma medications used.9,10,13–15 However, PAS also develop after µLOT, which we reported after evaluating images of the entire circumference anterior chamber angle using the Gonioscope GS-1 (NIDEK Co., Gamagori, Japan).16 Postoperative PAS formation after µLOT also is not well studied; however, it could potentially reduce the surgical effectiveness and therefore would be clinically relevant. In addition, investigation of this would contribute to a better understanding of the surgical outcomes of trabeculotomy. Thus, the purpose of the current study was to investigate the prevalence, locations, and characteristics of PAS formation after µLOT using the GS-1 and evaluate the effects on various clinical variables including IOP and number of antiglaucoma medications.

Subjects and Methods

The current single-center retrospective observational case series was conducted at Shimane University Hospital, Izumo, Japan, as a part of the study protocol “Establishment of New Gonioscopic Methods and Development of Automatic Analysis Algorithms Using 360–degree Gonioscopic Images.” The institutional review board (IRB) of Shimane University Hospital reviewed and approved the research (No. 20181124-1). All research adhered to the tenets of the Declaration of Helsinki. The IRB approval did not require each patient to provide written informed consent for publication; instead, the study protocol was posted at the study institutions to notify participants about the study, and they were given the opportunity to opt out from the research.

Participants

One hundred eight eyes of 75 consecutive subjects with open-angle glaucoma were identified in a search of the medical record data and enrolled; the eyes had 360 degrees of visible TM preoperatively and had undergone µLOT or combined µLOT and cataract surgery. One surgeon (MT) performed all the cataract surgeries and the initial glaucoma surgery, and all had been performed successfully using the gonioscope GS-1 between October 2018 to August 2019 at Shimane University Hospital. Eyes were excluded if they had PAS preoperatively, active uveitis or a history of uveitis, intraocular inflammation, trauma, and an eye surgery that included cataract extraction and laser treatment, which might have affected postoperative PAS formation. All patients were Japanese and data from both eyes were included. In addition to patient age, gender, ocular characteristics of each study eye, and clinical diagnosis, the following examination data were obtained from the medical chart review: visual acuity; findings from slit-lamp, manual gonioscopy, and fundus examinations; refractive error by auto-refractometry (RC-5000, Tomey Corporation, Nagoya, Japan); IOP measured by Goldmann applanation tonometry (Haag–Streit, Koniz, Switzerland); visual field mean deviation of the central 30-2 program by Humphrey Field Analyzer (HFA) (Carl Zeiss Meditec, Dublin, CA, USA); axial length and central anterior chamber depth (ACD) measured by optical biometry (OA-2000, Tomey Corporation); and anterior chamber aqueous flare measured by FM-600 laser flare meter (Kowa, Nagoya, Japan). The patients with primary open-angle glaucoma (POAG) had glaucomatous optic nerve damage with a normal-appearing anterior chamber angle and visual function loss on HFA.17 The patients with pseudoexfoliation glaucoma had a history of IOP elevation that exceeded 22 mmHg without treatment because of pseudoexfoliation material.18 The patients with juvenile open-angle glaucoma had increased IOP or a history of IOP elevation exceeding 22 mmHg without treatment, glaucomatous optic nerve damage for a primary reason excluding all secondary causes, and visual function loss on perimetry. The patients had been diagnosed between the ages of 3 and 40 years.19 The exclusion criteria were the inability to obtain clear GS-1 gonio-photographs, which prevented reliable examination for the presence of PAS. After excluding three eyes of three subjects because of poor-quality images, 105 eyes of 75 participants were analyzed.

Gonioscope GS-1 Imaging

The Gonioscope GS-1 is a recently released anterior-segment imaging device that covers 360 degrees of the angle and provides true-color gonio-images automatically in a standardized manner in less than 1 minute/eye. Because these images can be analyzed post-hoc, physicians can make detailed observations and magnify any abnormalities. According to our previous reports,20,21 after applying topical anesthetic eye drops and using gel coupling, gonio-images of the entire circumference were captured by GS-1 with the participants fixating in primary gaze in a darkened room as during a standard evaluation. The angle was detected using a prism with 16 mirrored facets, which covers about 30 degrees each, and a series of 16 images was acquired as if performing indirect gonioscopy with a Goldmann lens. The instrument then automatically displayed the image with the best focus within the 17 depths of focus for each position. Finally, we acquired the 16 gonio-images with the best focus for each eye. Figure 1A shows a typical merged GS-1 image displayed in a 360-degree view of the angle.

Figure 1

Representative gonioscope GS-1 images with PAS formation after µLOT in a 360-degree view of the angle, and the distribution of the average PAS formation rate in each angle position.

Microhook Ab-interno Trabeculotomy

A standard surgical procedure was performed with microhooks during µLOT. A Swan-Jacob gonioprism lens (Ocular Instruments, Inc., Bellevue, WA) was used to observe the angle opposite to the corneal port, and the microhook was inserted into the anterior chamber through the corneal port. The microhook tip then was inserted into Schlemm’s canal and moved circumferentially to incise the inner wall of the canal and TM over 90-degree extents; this also was performed on the opposite side. For postoperative eye drops,1.5% levofloxacin (Nipro, Osaka, Japan) and 0.1% betamethasone (Sanbetason, Santen Pharmaceutical) are applied topically 4 times daily for 3–4 weeks in all cases. Miotic ophthalmic solutions are not used postoperatively.16 Regarding the clinical records, all subjects had undergone successful standard µLOT procedures, and the incisions were created that extended from 180 to 240 degrees, with at least the following sectors of the TM incised: the superotemporal (ST) to superotemporal-temporal (STT)∼inferotemporal (IT) to inferoinferior-temporal (IIT) and inferoinferior nasal (IIN) to inferonasal (IN)∼superonasal-nasal (SNN) to superonasal (SN). Therefore, eight images of the STT, T, ITT, IT, IN, INN, N, and SNN sectors were included in the extend of the µLOT incisions (Figure 1B).

Postoperative PAS Evaluations with GS-1 Gonio-Images

We assessed the prevalence of PAS formation after trabeculotomy and the rates of postoperative PAS formation in the total angle circumference both within the extent of the incision and outside of the incision in the 16 GS-1 gonio-images of each eye. An ophthalmologist (MM) who was masked to the study and familiar with the angle determinations using the GS-1 evaluated the PAS formation in each gonio-photograph. We then calculated the rate of PAS formation as follows: total = X images/16 images; rate of PAS formation in the extent of the µLOT incision = Y images/8 images; and the rate of PAS formation outside of the µLOT incision = Z images/8 images. Figure 1A shows a representative image of the GS-1 with PAS formation postoperatively, and it was determined that X = 5, Y = 5, and Z = 0 in this case.

Statistical Analysis

All statistical analyses were performed using the JMP Pro version 14.2 statistical software (SAS Institute, Inc., Cary, NC, USA). The clinical and demographic characteristics were expressed as the means and standard deviations (SD) for continuous variables or by the number and frequency of discrete variables. The differences in the continuous variables were evaluated statistically between preoperatively and postoperatively in the study population using the paired t-test. The incidence of PAS formation and rates after µLOT were classified based on the categorical variables in the demographic data and then analyzed using the Pearson’s chi-square test for categorical data and the Student’s t-test for continuous data or the Pearson’s chi-square test for categorical data and one-way analysis of variance for continuous data. The paired t-test also was used to analyze the differences between the PAS formation rates within and outside of the incisions. Moreover, the possible associations among the PAS rate and various variables were analyzed by a simple linear regression model and a mixed-effect model. P < 0.05 was considered statistically significant.

Results

A total of 105 eyes of 75 participants were analyzed. Of the 105 eyes with an open angle, 68 had POAG, 27 had pseudoexfoliation glaucoma, and 10 had juvenile open-angle glaucoma. Seventy-nine eyes had undergone µLOT combined with uncomplicated phacoemulsification and intraocular lens implantation, and the other 26 eyes had undergone µLOT alone. Table 1 shows the continuous variables preoperatively and postoperatively in the study subjects. All participants were Japanese. The mean age (± SD) was 64.1 ± 15.6 years. PAS formed in 90 (86%) eyes postoperatively. The mean numbers (± SD) of images that showed postoperative PAS formation in all eyes were 4.1 ± 3.2, within the µLOT incision 3.1 ± 2.3, and outside of the µLOT incision 1.0 ± 1.3. Figure 1B shows the distribution of the average PAS formation rate in each sector of the GS-1 gonio-images.

Table 1

Continuous Preoperative and Postoperative Variables

Variable	Preoperative Conditions (n=105)	Postoperative Conditions (n=105)	p*
Baseline characteristics
Age, years, mean ± SD (range)	64.1±15.6 (11–87)	NA
Mean Shaffer grade, mean ± SD (range)	3.5±0.72 (1–4)	NA
Axial length, mm, mean ± SD (range)	24.9±2.0 (21.2–30.7)	NA
Visual field mean deviation, dB, mean ± SD (range)	−12.6±8.4 (−29.71–1.16)	NA
Postoperative days, mean ± SD (range)	0	225±226 (14–1021)	<0.01
LogMAR best corrected visual acuity, mean ± SD (range)	0.19±0.43 (−0.08–2.30)	0.11±0.39 (−0.08–2.30)	<0.01
Intraocular pressure, mmHg, mean ± SD (range)	20.7±6.7 (12.0–51.0)	13.7±3.7 (6–21)	<0.01
Central anterior chamber depth, mm, mean ± SD (range)	3.28±0.42 (2.06–4.77)	3.69±0.67 (2.33–5.41)	<0.01
Aqueous flare, pc/ms, mean ± SD (range)	9.1±5.0 (2.6–27.9)	15.3±14.5 (2.3–121.3)	<0.01
Topical glaucoma medications, mean ± SD (range)	3.3±0.9 (1–5)	2.7±0.8 (0–4)	<0.01
Primary outcome variables
Peripheral anterior synechiae formation, number (%)	0	90 (86)	<0.01
Images of peripheral anterior synechiae formation in total, mean ± SD (range)	0	4.1±3.2 (0–14)	<0.01
Images of peripheral anterior synechiae formation in incision extent of µLOT, mean ± SD (range)	0	3.1±2.3 (0–8)	<0.01
Images of peripheral anterior synechiae formation in non–incision extent of µLOT, mean ± SD (range)	0	1.0±1.3 (0–7)	<0.01

Note: Statistical significance was analyzed by the paired t-test (*).

Abbreviations: SD, standard deviation; µLOT, microhook ab interno trabeculotomy; n, number of eyes; NA, not applicable; dB, decibels; logMAR, logarithm of the minimum angle of resolution; pc/ms, photon counts per millisecond.

Table 2 shows the postoperative PAS formation frequencies and rates classified according to the categorical variables in the demographic data. In the group that underwent the combined surgery, the total PAS rate was significantly higher than that in the µLOT alone group (p=0.03). The PAS frequencies also differed significantly among the glaucoma types (p=0.03). Thus, we assumed that regardless of whether the combined surgery or solo surgery was performed, the glaucoma type could be related to PAS formation.

Table 2

PAS Formation Frequencies and Rates After µlot Classified by Categorical Variables in Demographic Data

Variable	PAS Number (%)	Total PAS Rate, Mean ± SD	Rate Within µLOT Incision, Mean ± SD	Rate Outside of µLOT Incision, Mean ± SD	p‡
All study subjects (n=105)	90 (86)	0.26±0.20	0.39±0.29	0.13±0.16	<0.0001
Sex
Male (n=60)	53 (88)	0.26±0.18	0.39±0.27	0.13±0.15	<0.0001
Female (n=45)	37 (82)	0.26±0.23	0.38±0.32	0.14±0.18	<0.0001
p*	0.38	0.96	0.81	0.74
Right or left eye
Right eye (n=53)	46 (87)	0.26±0.20	0.38±0.28	0.14±0.17	<0.0001
Left eye (n=52)	44 (85)	0.26±0.20	0.39±0.30	0.13±0.16	<0.0001
p*	0.75	0.96	0.84	0.76
µLOT combined with cataract surgery
Yes (n=79)	65 (82)	0.23±0.20	0.36±0.30	0.11±0.15	<0.0001
No (n=26)	25 (96)	0.33±0.19	0.48±0.24	0.19±0.19	<0.0001
p*	0.08	0.03	0.07	0.09
Glaucoma type
POAG (n=68)	62 (91)	0.25±0.17	0.39±0.27	0.11±0.12	<0.0001
PEG (n=27)	19 (70)	0.26±0.24	0.38±0.35	0.15±0.20	0.0005
JOAG (n=10)	9 (90)	0.29±0.25	0.38±0.28	0.20±0.26	0.02
p†	0.03	0.86	0.96	0.23

Notes: Statistical significance was analyzed by the chi-square test for categorical data and the Student’s t-test for continuous data (*); by the chi-square test for categorical data and one-way analysis of variance for continuous data (†); by the paired t-test (‡).

Abbreviations: PAS, peripheral anterior synechiae; µLOT, microhook ab-interno trabeculotomy; n, number of eyes; POAG, primary open angle glaucoma; PEG, pseudoexfoliation glaucoma; JOAG, juvenile open angle glaucoma; SD, standard deviation.

Table 3 shows the possible associations among the PAS rates and various continuous variables analyzed by the simple linear regression model. The preoperative mean Shaffer grade, preoperative axial length, and preoperative and postoperative central ACD were negatively associated with the total PAS rate (p<0.05, respectively). The preoperative central ACD had a higher negative regression coefficient with the total PAS rate, with a lower p value than the postoperative central ACD. The postoperative days and preoperative topical glaucoma medications were positively correlated with the total PAS rate (p<0.05 for both comparisons). Thus, we considered that the preoperative mean Shaffer grade, preoperative axial length, preoperative central ACD, postoperative days, and preoperative topical glaucoma medications also could be related to PAS formation.

Table 3

Possible Associations Among PAS Rates and Various Continuous Variables Analyzed by the Simple Linear Regression Model

Variable	Preoperative Conditions (n=105)						Postoperative Conditions (n=105)
	Total PAS Rate		PAS Rate Within µLOT Incision		PAS Rate outside of µLOT Incision		Total PAS rate		PAS Rate Within µLOT Incision		PAS Rate Outside of µLOT Incision
	r (95% CI)	p*	r (95% CI)	p*	r (95% CI)	p*	r (95% CI)	p*	r (95% CI)	p*	r (95% CI)	p*
Age (/year)	−0.0016	0.20	−0.0016	0.38	−0.0016	0.12	NA	NA	NA	NA	NA	NA
Mean Shaffer grade	−0.058	0.03	−0.070	0.08	−0.046	0.04	NA	NA	NA	NA	NA	NA
Axial length (/mm)	−0.023	0.02	−0.028	0.053	−0.019	0.02	NA	NA	NA	NA	NA	NA
Visual field mean deviation (/dB)	−0.0012	0.62	−0.0021	0.55	−0.00028	0.89	NA	NA	NA	NA	NA	NA
Postoperative days (/day)	NA	NA	NA	NA	NA	NA	0.00022	0.01	0.00034	<0.01	0.000096	0.18
LogMAR best-corrected visual acuity	0.060	0.18	0.12	0.07	0.00048	0.99	0.036	0.48	0.093	0.21	−0.020	0.63
Intraocular pressure (/mmHg)	0.00046	0.88	0.0050	0.25	−0.0040	0.10	−0.0055	0.23	−0.0052	0.43	−0.0056	0.13
Central anterior chamber depth (/mm)	−0.17	<0.01	−0.19	<0.01	−0.15	<0.01	−0.068	0.02	−0.096	0.03	−0.040	0.10
Aqueous flare (/pc/ms)	0.0012	0.77	0.00071	0.90	0.0016	0.63	−0.00050	0.71	−0.0018	0.38	0.00075	0.50
Topical glaucoma medications	0.062	<0.01	0.097	<0.01	0.027	0.12	0.024	0.32	0.056	0.11	−0.0066	0.74

Note: Possible association was analyzed by the simple linear regression model (*).

Abbreviations: PAS, peripheral anterior synechiae; n, number of eyes; µLOT, microhook ab-interno trabeculotomy; SD, standard deviation; r, regression coefficient; NA, not applicable; logMAR, logarithm of the minimum angle of resolution; dB, decibels; CI, confidence interval; pc/ms, photon counts per millisecond.

Table 4 shows the possible associations among the PAS rate and various variables analyzed by the mixed-effect model that was performed with the eye as the unit of analysis, with a fixed effect for the possible related factors detected in Tables 2 and 3, and with crossed random effects to adjust for a correlation with the fellow eyes. We also added the preoperative IOP to the analysis as a fixed effect, which should be strongly correlated with the preoperative topical glaucoma medications. Finally, we found that the total PAS rate and the rate within the incision were correlated positively with the postoperative days (p=0.01 and 0.004, respectively), that the extent outside of the incision was correlated positively with µLOT alone, and that there was a negative correlation with the preoperative central ACD (p=0.048 and 0.04, respectively).

Table 4

Possible Associations Among PAS Rate and Various Variables Analyzed by the Mixed-Effect Model

Variable	Total PAS Rate (n=105)		PAS Rate Within µLOT Incision (n=105)		PAS Rate Outside of µLOT Incision (n=105)
	Estimate (95% CI)	p*	Estimate (95% CI)	p*	Estimate (95% CI)	p*
Postoperative days (/day)	0.00022	0.01	0.00037	0.004	0.000078	0.29
µLOT combined with cataract surgery (/yes)	0.062	0.06	0.064	0.17	0.054	0.048
Preoperative central anterior chamber depth (/mm)	−0.11	0.07	−0.12	0.19	−0.11	0.04
Preoperative topical glaucoma medications	0.031	0.19	0.060	0.08	0.0056	0.77
Axial length (/mm)	−0.013	0.28	−0.014	0.41	−0.0093	0.35
Mean Shaffer grade	−0.033	0.35	−0.054	0.29	−0.0079	0.79
Preoperative intraocular pressure (/mmHg)	0.0028	0.42	0.0092	0.07	−0.0035	0.23
Glaucoma type (JOAG/POAG)	0.016	0.78	0.012	0.89	0.022	0.65
Glaucoma type (PEG/POAG)	−0.0052	0.90	−0.024	0.70	0.0086	0.81

Notes: Possible association was analyzed by the mixed-effect model with crossed random effects to adjust for correlation between fellow eyes (*).

Abbreviations: PAS, peripheral anterior synechiae; µLOT, microhook ab-interno trabeculotomy; POAG, primary open angle glaucoma; JOAG, juvenile open angle glaucoma; PEG, pseudoexfoliation glaucoma; n, number of eyes; CI, confidence interval.

Discussion

The current study investigated the prevalence, location, and related characteristics of PAS formation after µLOT using gonioscope GS-1 images that should the details of the entire circumference of the angle and analyzed their effects on clinical variables including the IOP and number of antiglaucoma medications.

Compared to baseline, examinations 225 days postoperatively showed that the IOP decreased significantly by 34% from 20.7 to 13.7 mmHg. The number of antiglaucoma medications also decreased significantly by 18% from 3.3 to 2.7. The 6‑month postoperative examination showed that the IOP decreased significantly by 43% from 25.9 to 14.7 mmHg as the result of µLOT alone and the number of antiglaucoma medications decreased by 15% from 3.3 to 2.8.14 At the 9.5‑month examination after µLOT combined with cataract surgery, the IOP decreased significantly by 28% from 16.4 to 11.8 mmHg, and the number of antiglaucoma medications decreased by 13% from 2.4 to 2.1.15 Moreover, a recent study reported the significant continuous effects of the combined procedures on reducing the IOP and the number of antiglaucoma medications at all time points between 1 and 6 months postoperatively.10 Therefore, similar to previous reports, µLOT alone or µLOT combined with cataract surgery resulted in significant decreases in the IOP and number of antiglaucoma medications during follow-up examinations.

We initially evaluated PAS formation after trabeculotomy in detail using GS-1 gonio-images that cover 360 degrees of the angle. Tables 1 and 2 show that PAS developed after µLOT in 86% of patients and the total formation rate was 0.26. In addition, the rate was significantly higher within the µLOT incision (rate, 0.39) than outside of the incision (rate, 0.13). Figure 1B is a radar chart that shows the distribution of the average PAS formation rates in each sector on the 360-degree gonio-images of the GS-1. The results could be generalized in PAS formation after the other trabeculotomy-related glaucoma surgeries. Moreover, the methodology would be useful to objectively assess the postoperative angle changes.

The results of the current analyses indicated that the total PAS rate and the rate within the incision may be associated with the postoperative days, that PAS formation outside of the incision may be associated with the combined surgery or not and with the preoperative central ACD, which might help elucidate the etiology and pathophysiology of PAS formation in different conditions. Synechiae are thought to form during two different conditions, ie, in association with inflammation and cellular proliferation and in association with non-proliferation. The pathophysiology of the former would be related to inflammatory cells, fibrin, and protein deposition, which stimulate the formation of adhesions between structures. However, in the latter condition, PAS may result from iris apposition on the TM, which includes trauma and increased IOP.22 Therefore, the total PAS and PAS within the µLOT incision would develop gradually over time postoperatively because of the continuous increased outflow into Schlemm’s canal within the incision, which induces iris apposition on the TM. Inflammation associated with surgery might contribute to PAS formation; however, we did not detect this because aqueous flare, which is used to estimate postoperative inflammation, was not associated significantly with the PAS rate. Accordingly, inflammation can be associated with formation of PAS during early postoperative periods rather than the gradual increase of PAS during later periods. While aqueous outflow into the area of the TM that was not incised during µLOT should not increase, PAS in the non-incised area may have developed due to the shallow postoperative ACD, which also stimulates iris apposition on the TM. Therefore, a deep preoperative ACD and combined cataract surgery, which deepen the postoperative ACD, would be significantly negatively correlated with the PAS rate in the current study. Accordingly, PAS within the incision and PAS in the non-incised area might result from different causes.

We did not detect a correlation between the rate of PAS formation and a therapeutic effect by µLOT; however, several studies have reported a relationship between PAS and increased IOP or attenuation of the IOP–lowering effect as a result of treatment.23–25 One reason may be that the total PAS rate (rate, 0.26) was not sufficient to increase the IOP. Moreover, the actual extent of the PAS would be shorter than the estimated PAS rate because we calculated the rate based on the presence of PAS in each gonio-image, which could be overestimated. Thus, we should study this hypothesis in a future study that measures the actual extent of the PAS.

The current study had other limitations. First, postoperative observation was performed at only one point for a relatively a short period and the time points of observation were not consistent because of the retrospective observational nature of the study. Although we did not detect a significant difference in the relationship, we cannot deny that possibility. Second, the study included relatively few patients, so the statistical power might have been insufficient to detect all factors involved in PAS formation. Finally, our study might have limited generalizability because of the exclusion of the poor-quality images. Therefore, further studies are needed to reach a definitive conclusion about the relationships between PAS after µLOT and the IOP-lowering effect.

Conclusion

The current study identified postoperative PAS formation after µLOT in most patients and the rate was significantly higher within the incision compared with the rate outside of the incision. A radar chart also showed the distribution of the average PAS formation rate in each sector. Moreover, we showed that the total PAS rate and the rate within the incision may be associated with the postoperative days, that in the area not incised there was a possible association with combined surgery or not and with preoperative central ACD in the mixed-effect model analysis, which suggested that PAS within the incision and the PAS in the area outside the incision would develop from different causes. However, while further studies are needed, the current study contributes knowledge about the surgical outcomes of trabeculotomy-related glaucoma surgeries.