Shinichi Yamazaki1, Hossein Mardani-Korrani2, Rumi Kaida2, Kumiko Ochiai3, Masaru Kobayashi3, Atsushi J Nagano4, Yoshiharu Fujii2, Akifumi Sugiyama5, Yuichi Aoki6. 1. Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan. 2. Department of International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan. 3. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. 4. Faculty of Agriculture, Ryukoku University, Otsu, Japan. 5. Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Japan. 6. Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan. aokibluetree@gmail.com.
Abstract
The plant root-associated environments such as the rhizosphere, rhizoplane, and endosphere are different from the outer soil region (bulk soil). They establish characteristic conditions including microbiota, metabolites, and minerals, and they can directly affect plant growth and development. However, comprehensive insights into those characteristic environments, especially the rhizosphere, and molecular mechanisms of their formation are not well understood. In the present study, we investigated the spatiotemporal dynamics of the root-associated environment in actual field conditions by multi-omics analyses (mineral, microbiome, and transcriptome) of soybean plants. Mineral and microbiome analyses demonstrated a characteristic rhizosphere environment in which most of the minerals were highly accumulated and bacterial communities were distinct from those in the bulk soil. Mantel's test and co-abundance network analysis revealed that characteristic community structures and dominant bacterial taxa in the rhizosphere significantly interact with mineral contents in the rhizosphere, but not in the bulk soil. Our field multi-omics analysis suggests a rhizosphere-specific close association between the microbiota and mineral environment.
The plant root-associated envclass="Chemical">ironments such as the rhizosclass="Chemical">phere, rhizoclass="Chemical">plane, and endosclass="Chemical">phere are difclass="Chemical">pan class="Chemical">ferent from the outer soil region (bulk soil). They establish characteristic conditions including microbiota, metabolites, and minerals, and they can directly affect plant growth and development. However, comprehensive insights into those characteristic environments, especially the rhizosphere, and molecular mechanisms of their formation are not well understood. In the present study, we investigated the spatiotemporal dynamics of the root-associated environment in actual field conditions by multi-omics analyses (mineral, microbiome, and transcriptome) of soybean plants. Mineral and microbiome analyses demonstrated a characteristic rhizosphere environment in which most of the minerals were highly accumulated and bacterial communities were distinct from those in the bulk soil. Mantel's test and co-abundance network analysis revealed that characteristic community structures and dominant bacterial taxa in the rhizosphere significantly interact with mineral contents in the rhizosphere, but not in the bulk soil. Our field multi-omics analysis suggests a rhizosphere-specific close association between the microbiota and mineral environment.
Authors: Joseph Edwards; Cameron Johnson; Christian Santos-Medellín; Eugene Lurie; Natraj Kumar Podishetty; Srijak Bhatnagar; Jonathan A Eisen; Venkatesan Sundaresan Journal: Proc Natl Acad Sci U S A Date: 2015-01-20 Impact factor: 11.205