Eduardo Anitua1, Andreia Cerqueira2, Francisco Romero-Gavilán2, Iñaki García-Arnáez3, Cristina Martinez-Ramos4, Seda Ozturan5, Mikel Azkargorta6, Félix Elortza6, Mariló Gurruchaga3, Isabel Goñi3, Julio Suay2, Ricardo Tejero7. 1. University Institute of Regenerative Medicine and Oral Implantology (UIRMI), University of the Basque Country (UPV-EHU), C/ Jacinto Quincoces, 39, 01007, Vitoria, Spain. 2. Department of Industrial Systems Engineering and Design, Universitat Jaume I, Av. Vicent Sos Baynat s/n, 12071, Castellón de la Plana, Spain. 3. Faculty of Chemical Sciences, University of the Basque Country (UPV-EHU), P.M. de Lardizábal, 3, 20018, San Sebastián, Spain. 4. Center for Biomaterials and Tissue Engineering, Universitat Politècnica de Valencia, Camino de Vera, s/n, 46022, Valencia, Spain. 5. Department of Periodontology, Faculty of Dentistry, Istambul Medeniyet University, Istanbul, Turkey. 6. Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII, Bizkaia Science and Technology Park, 48160, Derio, Spain. 7. University Institute of Regenerative Medicine and Oral Implantology (UIRMI), University of the Basque Country (UPV-EHU), C/ Jacinto Quincoces, 39, 01007, Vitoria, Spain. ricardo@minin.es.
Abstract
BACKGROUND: Calcium (Ca) is a well-known element in bone metabolism and blood coagulation. Here, we investigate the link between the protein adsorption pattern and the in vivo responses of surfaces modified with calcium ions (Ca-ion) as compared to standard titanium implant surfaces (control). We used LC-MS/MS to identify the proteins adhered to the surfaces after incubation with human serum and performed bilateral surgeries in the medial section of the femoral condyles of 18 New Zealand white rabbits to test osseointegration at 2 and 8 weeks post-implantation (n=9). RESULTS: Ca-ion surfaces adsorbed 181.42 times more FA10 and 3.85 times less FA12 (p<0.001), which are factors of the common and the intrinsic coagulation pathways respectively. We also detected differences in A1AT, PLMN, FA12, KNG1, HEP2, LYSC, PIP, SAMP, VTNC, SAA4, and CFAH (p<0.01). At 2 and 8 weeks post-implantation, the mean bone implant contact (BIC) with Ca-ion surfaces was respectively 1.52 and 1.25 times higher, and the mean bone volume density (BVD) was respectively 1.35 and 1.13 times higher. Differences were statistically significant for BIC at 2 and 8 weeks and for BVD at 2 weeks (p<0.05). CONCLUSIONS: The strong thrombogenic protein adsorption pattern at Ca-ion surfaces correlated with significantly higher levels of implant osseointegration. More effective implant surfaces combined with smaller implants enable less invasive surgeries, shorter healing times, and overall lower intervention costs, especially in cases of low quantity or quality of bone.
BACKGROUND:Calcium (Ca) is a well-known element in bone metabolism and blood coagulation. Here, we investigate the link between the protein adsorption pattern and the in vivo responses of surfaces modified with calcium ions (Ca-ion) as compared to standard titanium implant surfaces (control). We used LC-MS/MS to identify the proteins adhered to the surfaces after incubation with human serum and performed bilateral surgeries in the medial section of the femoral condyles of 18 New Zealand white rabbits to test osseointegration at 2 and 8 weeks post-implantation (n=9). RESULTS: Ca-ion surfaces adsorbed 181.42 times more FA10 and 3.85 times less FA12 (p<0.001), which are factors of the common and the intrinsic coagulation pathways respectively. We also detected differences in A1AT, PLMN, FA12, KNG1, HEP2, LYSC, PIP, SAMP, VTNC, SAA4, and CFAH (p<0.01). At 2 and 8 weeks post-implantation, the mean bone implant contact (BIC) with Ca-ion surfaces was respectively 1.52 and 1.25 times higher, and the mean bone volume density (BVD) was respectively 1.35 and 1.13 times higher. Differences were statistically significant for BIC at 2 and 8 weeks and for BVD at 2 weeks (p<0.05). CONCLUSIONS: The strong thrombogenic protein adsorption pattern at Ca-ion surfaces correlated with significantly higher levels of implant osseointegration. More effective implant surfaces combined with smaller implants enable less invasive surgeries, shorter healing times, and overall lower intervention costs, especially in cases of low quantity or quality of bone.
Authors: Stacey L Hirsh; David R McKenzie; Neil J Nosworthy; John A Denman; Osman U Sezerman; Marcela M M Bilek Journal: Colloids Surf B Biointerfaces Date: 2012-11-23 Impact factor: 5.268
Authors: N Araújo-Gomes; F Romero-Gavilán; I García-Arnáez; C Martínez-Ramos; A M Sánchez-Pérez; M Azkargorta; F Elortza; J J Martín de Llano; M Gurruchaga; I Goñi; J Suay Journal: J Biol Inorg Chem Date: 2018-03-23 Impact factor: 3.358
Authors: Jakub Litak; Michał Szymoniuk; Wojciech Czyżewski; Zofia Hoffman; Joanna Litak; Leon Sakwa; Piotr Kamieniak Journal: Materials (Basel) Date: 2022-05-20 Impact factor: 3.748