Entrectinib: A Review in NTRK+ Solid Tumours and ROS1+ NSCLC.

Entrectinib (Rozlytrek®) is an orally active, CNS-penetrant, small-molecule, selective inhibitor of the tropomyosin receptor tyrosine kinases TRKA/B/C [encoded by the neurotrophic tyrosine receptor kinase (NTRK) genes NTRK1/2/3, respectively], the proto-oncogene tyrosine-protein kinase ROS1 (ROS1) and the anaplastic lymphoma kinase gene (ALK). It is approved for the treatment of adults and paediatric patients aged ≥ 12 years with NTRK fusion-positive (NTRK+) solid tumours and adults with ROS1 fusion-positive (ROS1+) non-small-cell lung cancer (NSCLC). In trials in adults, entrectinib induced clinically meaningful and durable systemic responses in tyrosine kinase inhibitor (TKI)-naïve patients with locally-advanced or metastatic NTRK+ solid tumours or ROS1+ NSCLC, irrespective of the presence or absence of CNS metastases at baseline. Moreover, entrectinib demonstrated substantial intracranial efficacy in patients with baseline CNS metastases. Entrectinb efficacy in paediatric patients was established on the basis of extrapolation of clinical trial data from adults with NTRK+ solid tumours and children and adolescents aged < 21 years with recurrent or refractory NTRK+ CNS/solid tumours. Entrectinib was generally well tolerated, with a manageable safety profile. Thus, entrectinib expands the range of treatment options for advanced NTRK+ solid tumours and ROS1+ NSCLC, and may be of particular value in patients with existing CNS metastases and those who are at risk of developing CNS metastases.

Entrectinib: clinical considerations in NTRK+ solid tumours and ROS1+ NSCLC

Introduction

The diagnosis and treatment of solid tumours has firmly entered the era of personalized (or precision) medicine, with optimal patient management requiring molecular profiling aimed at identifying oncogenic driver mutations that are actionable or potential therapeutic targets [1-4]. In this regard, gene rearrangements (fusions) involving the neurotrophic tyrosine receptor kinase (NTRK) genes NTRK1, NTRK2 and NTRK3 [encoding the tropomyosin receptor kinase (TRK) receptors TRKA, TRKB and TRKC, respectively], the c-ros oncogene 1 receptor tyrosine kinase (ROS1) gene [encoding proto-oncogene tyrosine-protein kinase ROS (ROS1)] and the anaplastic lymphoma kinase gene (ALK; encoding the ALK receptor tyrosine kinase) are variously implicated, albeit often infrequently, in a broad range of (> 40) solid tumour types [5]. NTRK fusions, which are thought to be present in up to 1% of all solid tumours, are found in a wide variety of adult and paediatric tumour types; they are extremely common (incidence > 90%) in rare cancer types, such as infantile fibrosarcoma and mammary analogue secretory carcinoma (MASC), but rare (incidence predominantly < 1%) in more common cancer types, such as lung, breast and colorectal cancers, and melanomas [2, 6, 7]. NTRK, ROS1 and ALK fusions are present in 0.1–1%, 1–2% and 4–6% of cases of non-small-cell lung cancer (NSCLC), respectively [8].

Among the commercially available, orally active, small-molecule tyrosine kinase inhibitors (TKIs), larotrectinib (a selective inhibitor of TRKA/B/C [9]) has been approved for the treatment of NTRK fusion-positive (NTRK+) solid tumours and crizotinib (an inhibitor of multiple tyrosine kinases, including ROS1 and ALK [10]) has been approved for the treatment of ROS1 fusion-positive (ROS1+) NSCLC. However, since larotrectinib has been intentionally designed to have limited CNS penetration [9] and crizotinib also has limited brain penetration [10], there is a need for additional treatments with effective intracranial (IC) activity against primary or secondary brain tumours harbouring NTRK or ROS1 fusions [11]. Regarding the latter, NSCLC is the most common cause of brain metastases [12]; at the time of diagnosis, brain metastases were present in 50% of patients with metastatic NTRK+ NSCLC [13] and 20–40% of patients with metastatic ROS1+ NSCLC [14, 15], according to small retrospective studies. Patients with CNS involvement have a poorer prognosis than those without IC disease [16, 17].

Against this background, the orally active, small-molecule TKI entrectinib (Rozlytrek®; a selective inhibitor of TRKA/B/C, ROS1 and ALK) has been designed to have systemic activity and also to cross the blood-brain barrier (BBB) and be retained within the CNS [18, 19]. Entrectinib has been approved for the treatment of adults and paediatric patients aged ≥ 12 years with NTRK+ solid tumours, as well as adults with ROS1+ NSCLC in several countries, including those of the EU (conditionally) [20], the USA (conditionally) [21], and Japan [22]. This article reviews pharmacological, therapeutic efficacy and tolerability data relevant to the use of entrectinib in these patient populations.

Pharmacodynamic Properties of Entrectinib

Entrectinib is a potent and selective adenosine triphosphate-competitive inhibitor of TRKA (IC50 value of 1.7 nmol/L), TRKB (0.1 nmol/L), TRKC (0.1 nmol/L), ROS1 (0.2 nmol/L) and ALK (1.6 nmol/L) in vitro [20, 23]. M5, the major and only active circulating metabolite of entrectinib (Sect. 3), showed similar in vitro potency and activity to the parent drug against TRK, ROS1 and ALK [20, 21].

In preclinical studies, entrectinib inhibited the growth of human tumour cell lines driven by NTRK1 or ALK fusions [18, 24] and that of engineered murine (Ba/f3) cell lines reliant on NTRK1/2/3, ROS1 or ALK fusions for survival [18, 24]; it was 40-times more potent at inhibiting the growth of ROS1-dependent Ba/f3 cells than crizotinib [24]. Entrectinib also displayed antitumour activity and induced tumour regression in patient-derived xenograft models of multiple tumour types harbouring NTRK, ROS1 or ALK fusions [18, 19, 24, 25]. Notably, entrectinib inhibited the proliferation of cancer cell lines and Ba/f3 cells harbouring NTRK1 kinase domain mutations that confer resistance to another TRK inhibitor, including the ‘gatekeeper’ residue substitution F589L and the xDFG motif substitution G667C [26].

Importantly, entrectinib is a weak P-glycoprotein (P-gp) substrate that can sustain CNS exposure, unlike crizotinib and larotrectinib, which are strong P-gp substrates with poor brain distribution [11]. In particular, entrectinib showed a weaker interaction than crizotinib and larotrectinib with P-gp in an in vitro assay using transfected porcine kidney epithelial cells overexpressing human P-gp, based on noticeably lower ‘apical efflux ratio’ (AP-ER) values (1.1 vs 3.5 and 2.8). In addition, and consistent with these relative AP-ER values, entrectinib (despite not reaching steady state) showed a more favourable CSF-to-plasma unbound concentration ratio than crizotinib and larotrectinib (both of which reached steady state) following constant intravenous infusion for 4–6 h in an in vivo brain distribution study (> 0.2 vs ≈ 0.03 and ≈ 0.03) [11]. Entrectinib demonstrated CNS penetration [5, 11] (e.g. steady-state brain/plasma ratios of 1.4–2.2, 0.6–1.0 and 0.4 following repeated daily oral administration in dog, rat and mouse, respectively [5]) and inhibited tumour growth and improved survival in murine models intracranially injected with human tumour cell lines driven by an NTRK [11] or ALK [24] fusion.

Resistance

Experience with other TKIs suggests that acquired resistance to entrectinib limiting its effectiveness will inevitably emerge, either as a result of an ‘on-target’ mechanism (i.e. a target kinase domain mutation) or, alternatively, other, ‘off-target’ mechanisms (e.g. a bypass signalling pathway) [27-29]. Accordingly, reports of patients with NTRK+ solid tumours acquiring resistance to entrectinib have identified both ‘on-target’ mechanisms (e.g. G595R and G667C mutations in LMNA-NTRK1+ metastatic colorectal carcinoma [27], G595R mutation in TPR-NTRK1+ pancreatic cancer [29] and G623R mutation in ETV6-NTRK3+ MASC [30]) and an ‘off-target’ mechanism (emergent MET amplification in PLEKHA6-NTRK1+ cholangiocarcinoma [29]). Similarly, mechanisms of acquired resistance to entrectinib in ROS1+ NSCLC reportedly include the target kinase domain mutations G2032R, F2004C and F2004I [31] and—as identified in in vitro models using patient-derived tumour cells—activation of MET-[32] and RAS-[28] mediated bypass signalling.

Based on a blood analysis from an ongoing, global, phase II basket study (STARTRK-2; Sect. 4), acquired resistance mutations were detected in 10 (34%) of the 29 evaluable patients in the NTRK+ solid tumour cohort and 5 (28%) of the 18 evaluable patients in the ROS1+ NSCLC cohort, all of which were target kinase domain mutations. One additional patient from each cohort, both of whom had a partial response (PR) to entrectinib prior to progression, showed the emergence of a mutation in an oncogene within the MAPK pathway [31].

Pharmacokinetic Properties of Entrectinib

The pharmacokinetics of entrectinib in adult patients with solid tumours, like those of its major active metabolite, M5, are linear [20, 33] and not dose- or time-dependent [20]. Peak plasma concentrations of entrectinib and M5 were seen ≈ 4–6 h following single and multiple doses of oral entrectinib in the fed state; steady-state concentrations of entrectinib and M5 were reached within 2 weeks [34]. The estimated steady-state accumulation ratios for entrectinib and M5 were 1.89 and 2.01, respectively [20, 33]. The bioavailability of entrectinib is not altered to clinically significant extent by food [18]. Both entrectinib and M5 are highly (> 99%) bound to human plasma proteins [20]. The estimated apparent volumes of distribution for entrectinib and M5 are 551 and 81.1 L, respectively [21].

Entrectinib is metabolized primarily by cytochrome P450 (CYP) 3A4 (≈ 76%) to M5; it is also conjugated by uridine 5′-diphospho-glucuronosyltransferase 1A4 to its N-glucuronide conjugate (M-11) [20]. Entrectinib is predominantly eliminated through hepatic metabolism; renal clearance has a minor role [83% of a radiolabelled oral dose was excreted in faeces (36% as unchanged entrectinib; 22% as M5); 3% was excreted in urine] [20, 34]. The estimated apparent clearances of entrectinib and M5 are 19.6 and 52.4 L/h, respectively; the estimated elimination half-lives are 20 and 40 h, respectively [20].

No clinically significant differences were seen in the pharmacokinetics of entrectinib, based on age (4–86 years), gender, bodyweight (32–130 kg), ethnicity (White, Asian and Black), mild hepatic impairment and mild to moderate renal impairment. The effects of moderate to severe hepatic impairment or severe renal impairment on pharmacokinetics of entrectinib are unknown [20]. In paediatric patients aged ≥ 12 years, exposure to entrectinib following once-daily doses of 400 mg [for body surface area (BSA) 1.11–1.50 m2) and 600 mg (for BSA ≥ 1.51 m2) is predicted to be similar to that seen in adults following once-daily doses of 600 mg [20].

Potential drug-drug interactions involving entrectinib, a weak P-gp substrate (Sect. 2) that is predominantly metabolized by CYP3A4, are summarized in Table 1.

Table 1

Summary of potential drug-drug interactions involving entrectinib [20]

Drug(s)	Potential interaction
CYP3A and P-gp inhibitors	Itraconazole (strong CYP3A inhibitor) ↑ ENT exposure by 600%; avoid coadministering ENT with moderate or strong CYP3A inhibitors (e.g. ritonavir, itraconazole). If coadministration unavoidable, ↓ ENT dose
	ENT is a weak P-gp substrate (in vitro); caution advised when coadministering it with moderate or strong P-gp inhibitors (e.g. verapamil, felodipine, paroxetine) due to risk of ↑ ENT exposure
CYP3A and P-gp inducers	Rifampin (strong inducer of both CYP3A and P-gp) ↑ ENT exposure by 77%; avoid coadministering ENT with moderate or strong CYP3A or P-gp inducers (e.g. carbamazepine, phenytoin, rifampin)
CYP3A and P-gp substrates	ENT is a weak CYP3A4 inhibitor; caution advised when coadministering it with sensitive CYP3A4 substrates that have a narrow therapeutic range (e.g. cisapride, ergotamine, quinidine) due to ↑ risk of adverse drug reactions
	ENT is a weak P-gp inhibitor. Its effect on coadministered digoxin (a sensitive P-gp substrate) is not clinically relevant, but it is currently unknown whether its effect on more sensitive oral P-gp substrates (e.g. dabigatran etexilate) is larger
Other transporter substrates	ENT is a BCRP inhibitor and a weak OATP1B1 inhibitor (in vitro); caution advised when coadministering it with sensitive oral BCRP substrates (e.g. methotrexate) or OATP1B1 substrates (e.g. atorvastatin) due to the risk of ↑ absorption

BCRP breast cancer resistance protein, CYP cytochrome P450, ENT entrectinib, OATP organic anion transporting polypeptide, P-gp P-glycoprotein, ↑ increased, ↓ decreased

Therapeutic Efficacy of Entrectinib

The efficacy of entrectinib, as monotherapy, has been demonstrated in pooled cohorts of adults aged ≥ 18 years with locally advanced (unresectable) or metastatic NTRK+ solid tumours or ROS1+ NSCLC, including those with CNS disease, who were treated in one of three, ongoing, open-label, noncomparative, multicentre studies: two phase I trials (ALKA-372-001 and STARTRK-1); and one phase II global basket trial (STARTRK-2) [17, 20, 35–37]. All patients included in these prespecified integrated analyses had measurable disease per Response Evaluation Criteria in Solid Tumours (RECIST) version 1.1, an Eastern Cooperative Oncology Group performance status (ECOG PS) of 0–2 and could have received previous cancer therapies, with the exception of TRK inhibitors (those with NTRK+ solid tumours) or ROS1 inhibitors (those with ROS1+ NSCLC) [17, 20, 35–37].

Eligible patients received entrectinib until disease progression or unacceptable toxicity; treatment could continue post-progression if the investigator decided that the patient continued to derive clinical benefit [35, 36]. Initial (primary) analyses involving patients with non-CNS primary, NTRK+ solid tumours (n = 54) [35] or ROS1+ NSCLC (n = 53) [36] who received at least one dose of entrectinib ≥ 600 mg once daily and who were followed up for a median of 12.9 and 15.5 months, respectively, have been published in full [35, 36]. However, data discussed in this section are derived from updated analyses involving larger populations of patients with non-CNS primary NTRK+ solid tumours (n = 74) [20, 37] (Sect 4.1) or ROS1+ NSCLC (n = 161) [17, 20] (Sect. 4.2) who received entrectinb 600 mg/day and who were followed up for a median of 14.2 and 15.8 months, respectively. Results have been reported in full [17] and in the EU summary of product characteristics (SPC) [20], the European Public Assessment Report (EPAR) [23] and abstracts/posters/oral presentations [16, 37–44].

As in the initial analyses [35, 36], the primary endpoints in the updated analyses [17, 20, 37] were objective response rate (ORR) and duration of response (DoR), as assessed by blinded independent central review (BICR) using RECIST v1.1. Secondary endpoints included IC ORR and DoR (by BICR) in patients with CNS metastases (asymptomatic or pretreated; measurable or non-measurable) at baseline per BICR [16, 17, 37]. Of note, time to CNS progression was assessed as a secondary and as an exploratory endpoint in patients with and without baseline CNS metastases per investigator, respectively. In this regard, patients with baseline CNS metastases per investigator underwent regular brain scans, whereas CNS follow-up of those without baseline CNS metastases per investigator was less comprehensive, being based on symptomatic progression or routine CNS scans where customary [16, 17].

NTRK+ Solid Tumours

In the updated integrated analysis, the efficacy of entrectinib was assessed in 74 adult patients with NTRK+ solid tumours [37]. The median age of the cohort was 57 years; 52.7% of patients were female, 70.3% were Caucasian, 17.6% were Asian and 86.4% had an ECOG PS ≤ 1 [37]. Nearly all patients had an NTRK1 (n = 30) or NTRK3 (n = 42) fusion [37]. Twelve different NTRK+ solid tumour types and > 25 histologies were represented, the most common cancers being sarcoma (21.6%), NSCLC (17.6%), MASC (17.6%), colorectal cancer (9.5%), thyroid cancer (9.5%) and breast cancer (8.1%) [37, 40]. The vast majority of patients (97.3%) had metastatic disease, the most common sites being lung (60.8%), lymph nodes (52.7%) and brain (25.7% per investigator/21.6% per BICR) [20, 37].

Entrectinib induced clinically meaningful and durable responses in patients with advanced solid tumours harbouring NTRK fusions, demonstrating both systemic efficacy (irrespective of the presence or absence of CNS metastases at baseline) [37] and IC efficacy (in patients with CNS metastases at baseline) [16]. In this respect, systemic and IC efficacy findings in the subset of Asian patients with NTRK+ solid tumours (n = 13) were consistent with those in the overall study cohort (n = 74) [44].

In the overall cohort, the systemic ORR was 63.5% [comprising five complete responses (CRs) and 42 PRs] and the median DoR was 12.9 months [20, 37] (Table 2). Based on Kaplan-Meier (KM) estimates, the 6-, 9- and 12-month durable response rates (DRRs) were 71%, 65% and 55%, respectively [20]. ORRs were consistent irrespective of baseline CNS disease status per BICR, although median DoR, median progression-free survival (PFS) and median overall survival (OS) were shorter in patients with baseline CNS metastases than those without baseline CNS metastases [37] (Table 2), which is expected, as the former have a poorer prognosis (Sect. 1). Most responses were reported by the time of the first post-baseline tumour assessment at 1 month [37].

Table 2

Efficacy of oral entrectinib 600 mg once daily in patients with NTRK fusion-positive solid tumours: pooled results from three ongoing phase I or II trials (ALKA-372-001; STARTRK-1 and STARTRK-2) [16, 37]

Pt population analysed (no. of pts)	Outcomesa
	ORRb (%)	CR (%)	PR (%)	DoRb (mo) [95% CI]	PFS (mo) [95% CI]	OS (mo) [95% CI]
Systemic efficacy
All pts (74)	63.5	6.8	56.8	12.9 [9.3–NE]	11.2 [8.0–15.7]	23.9 [16.0–NE]
Pts with BL CNS metastasesc (16)	62.5	0	62.5	6.0 [4.2–NE]	6.7 [4.7–NE]	14.3 [7.6–NE]
Pts without BL CNS metastasesc (58)	63.8	8.6	55.2	12.9 [9.3–NE]	12.0 [8.7–16.0]	23.9 [16.8–NE]
IC efficacyd
Pts with BL CNS metastasesc (16)	50.0	25.0	25.0	8.0 [6.7–NE]	8.9 [5.9–14.3]	NR
Pts with measurable BLc CNS metastases (8)	62.5	12.5	50.0	NE [5.0–NE]	10.1 [2.8–NE]	NR

BICR blinded independent central review, BL baseline, CR complete response, DoR duration of response, IC intracranial, NE not estimable, NR not reported, ORR objective response rate, OS overall survival, PFS progression-free survival, PR partial response, pt(s) patient(s), RECIST Response Evaluation Criteria In Solid Tumours

aEvaluated by BICR using RECIST v1.1, except OS. DoR, PFS and OS values are medians

b(Co-)primary endpoint

cPer BICR

dReported outcomes are IC-ORR (secondary endpoint), IC-CR, IC-PR, IC-DoR (secondary endpoint) and IC-PFS

Entrectinib showed activity across multiple NTRK+ solid tumour types, including sarcoma (ORR of 56.3%; n = 16) [20], NSCLC [ORR of 69.2% (one CR and eight PRs); n = 13] [20, 41], MASC (ORR of 92.3%; n = 13) [20] and various gastrointestinal cancers [ORR of 50.0% (all PRs); n = 12] [40]. Moreover, a beneficial effect was generally seen irrespective of prior therapy exposure (ORRs of 80.0%, 61.9%, 65.0% and 38.5% in patients who received 0, 1, 2 and ≥ 3 prior lines of systemic therapy in the metastatic setting, respectively) [39]. The relatively low ORR in the ≥ 3 prior lines of systemic therapy subgroup may be a reflection of a poorer prognosis population (i.e. 31% of patients in this subgroup versus 0%, 19% and 10% in the 0, 1 and 2 prior lines of systemic therapy subgroups, respectively, had an ECOG PS of 2) [39].

In terms of the CNS activity of entrectinib, the IC ORR in the subpopulation of eight patients with measurable baseline CNS metastases (including four who had received IC radiotherapy to the brain < 2 months prior to starting entrectinib) was 62.5%; the median IC DoR was not reached [16, 20] (Table 2). The IC ORR was also 62.5% (three CRs and two PRs) in the subpopulation of eight patients with NTRK+ NSCLC who had measurable or non-measurable baseline CNS metastases; it was 60% (three CR/PRs) in the subpopulation of five patients with NTRK+ NSCLC who had measurable baseline CNS metastases [38].

Rates of detected CNS progression confirmed by scans were very low; the median time to CNS progression was not estimable at the time of the data cut-off (31 October 2018) in the overall cohort or the subpopulations of patients with and without baseline CNS metastases per investigator [16]. 3 (15.8%) of the 19 patients with baseline CNS metastases per investigator experienced CNS progression, while none (0%) of the 55 patients without baseline CNS metastases per investigator developed new scan-confirmed brain lesions [16].

Patient-reported outcomes (PROs) were evaluated in 71 patients with NTRK+ solid tumours participating in the STARTRK-2 study [42]. At baseline, these patients reported moderate to high health-related quality of life (HR-QOL) and functioning, as assessed using the European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire (QLQ-C30). While receiving entrectinib, they reported improvements in HR-QOL and physical and role functioning (including a clinically meaningful improvement in role functioning), as well as maintenance of cognitive function. They also reported a clinically meaningful improvement in pain. Patients with NTRK+ NSCLC (n = 12) also completed the EORTC Quality of Life Questionnaire-Lung Cancer Module (QLQ-LC13). They reported a moderate lung cancer symptom burden at baseline; their symptoms were unchanged or improved during treatment with entrectinib, including a clinically meaningful improvement in cough. Similarly, colorectal cancer-related symptoms, such as bloating, were unchanged or improved over time, as assessed in seven patients using the EORTC Quality of Life Questionnaire-Colorectal Cancer Module (QLQ-CR29) [42].

Paediatric Patients

The efficacy of entrectinib in paediatric patients aged ≥ 12 years with NTRK+ solid tumours has been established on the basis of data extrapolation from adults with NTRK+ solid tumours enrolled in the ALKA-372-001, STARTRK-1 and STARTRK-2 studies (Sect. 4.1), and relevant data from children and adolescents aged < 21 years with recurrent or refractory NTRK+ CNS/solid tumours enrolled in another ongoing, open-label, non-comparative, multicentre, phase I/II study (STARTRK-NG) [20, 45].

At the most recent data cut-off (5 November 2019) [45], 39 patients with relapsed or refractory CNS/solid tumours, with or without NTRK, ROS1 or ALK fusions, had been enrolled and evaluated in STARTRK-NG, including 14 with NTRK fusions. Best responses per investigator (Response Assessment in Neuro-Oncology criteria) in 11 patients with NTRK+ CNS primary tumours were four CRs and three PRs. Three patients with NTRK+ primary CNS tumours had stable disease; one had disease progression. Best responses per investigator in three patients with NTRK+ extracranial solid tumours were two CRs and one PR. All responses occurred at entrectinib doses of ≥ 400 mg/m2 [45].

ROS1+ NSCLC

In the updated integrated analysis, the efficacy of entrectinib was assessed in 161 adult patients with ROS1+ NSCLC [17]. The median age of the cohort was 54 years; 65% of patients were female, 44% were Caucasian, 45% were Asian, 37% were current/former smokers and 90% had an ECOG PS ≤ 1 [17]. The vast majority of patients (97.5%) had adenocarcinoma; 98.1% had metastatic disease, with the most common sites being lymph nodes (69.6%), lungs (50.3%) and brain (34.8% per investigator/28.6% per BICR) [17, 20].

Entrectinib therapy was associated with clinically meaningful and durable responses in patients with ROS1+ NSCLC, showing both systemic antitumour activity (irrespective of the presence or absence of baseline CNS metastases) and IC antitumour activity (in patients with baseline CNS metastases) [17]. In this respect, systemic and IC efficacy findings in the subset of Asian patients with ROS1+ NSCLC (n = 41) were consistent with those in the overall study cohort (n = 161) [44].

In the overall cohort, the systemic ORR was 67.1% (comprising 14 CRs and 94 PRs) and the median DoR was 15.7 months [17] (Table 3). KM estimates of the 6-, 9- and 12-month DRRs were 83%, 75% and 63%, respectively [17]. Median PFS, like median DoR, was 15.7 months; median OS was not estimable at the time of the data cut-off [17] (Table 3). The 6-, 9- and 12-month PFS rates were 77%, 66% and 55%, respectively; the 6-, 9- and 12-month OS rates were 91%, 86% and 81%, respectively (KM estimates) [17].

Table 3

Efficacy of oral entrectinib 600 mg once daily in patients with ROS1 fusion-positive metastatic NSCLC: pooled results from three ongoing phase I or II trials (ALKA-372-001; STARTRK-1 and STARTRK-2) [17]

Pt population analyzed (no. of pts)	Outcomesa
	ORRb (%)	CR (%)	PR (%)	DoRb (mo) [95% CI]	PFS (mo) [95% CI]	OS (mo) [95% CI]
Systemic efficacy
All pts (161)	67.1	8.7	58.4	15.7 [13.9–28.6]	15.7 [11.0–21.1]	NE [28.3–NE]
Pts with BL CNS metastasesc (56)	62.5	7.1	55.4	14.9 [9.6–20.5]	11.8 [6.4–15.7]	28.3 [16.1–NE]
Pts without BL CNS metastasesc (105)	69.5	9.5	60.0	24.6 [13.9–34.8]	19.0 [12.0–29.6]	NE [30.8–NE]
IC efficacyd
Pts with BL CNS metastasese (46)	52.2	17.4	34.8	12.9 [7.1–22.1]	8.3 [6.4–15.7]	NR
Pts with measurable BL CNS metastasese (24)	79.2	12.5	66.7	12.9 [6.8–22.1]	12.0 [6.2–19.3]	NR

BICR blinded independent central review, BL baseline, CR complete response, DoR duration of response, IC intracranial, NE not estimable, NR not reported, ORR objective response rate, OS overall survival, PFS progression-free survival, pt(s) patient(s), PR partial response, RECIST Response Evaluation Criteria In Solid Tumours

aEvaluated by BICR using RECIST v1.1, except OS. DoR, PFS and OS values are medians

b(Co-)primary endpoint

cPer investigator

dReported outcomes are IC-ORR (secondary endpoint), IC-CR, IC-PR, IC-DoR (secondary endpoint) and IC-PFS

ePer BICR

ORRs were consistent irrespective of investigator-assessed CNS disease status at baseline, although median DoR and median PFS were, as expected, shorter in patients with baseline CNS metastases who have a poorer prognosis than those without baseline CNS metastases [17] (Table 3; Sect. 1). Median OS was not estimable at the time of the data cut-off in the overall cohort or the subpopulation of patients without baseline CNS metastases; it was 28.3 months in the subpopulation of patients with baseline CNS metastases [17] (Table 3).

Responses occurred early, with most reported at the first follow-up tumour assessment (1 month) [17]. Additionally, ORR benefit was observed to be generally irrespective of prior therapy exposure, being 71.7%, 60.9%, 66.7% and 73.7% in patients who received 0, 1, 2 and ≥ 3 prior lines of systemic therapy in the metastatic setting, respectively [39].

In terms of the CNS activity of entrectinib, the IC ORR in the subpopulation of 24 patients with measurable baseline CNS metastases (including nine who had received IC radiotherapy to the brain < 2 months before initiating entrectinib) was 79.2%; the median IC DoR was 12.9 months (Table 3). KM estimates of the 6-, 9- and 12-month IC DRRs were 77%, 61% and 55%, respectively [17]. The median time to radiologically-confirmed CNS progression was not estimable at the time of the data cut-off (1 May 2019) in the overall cohort or the subpopulation of patients without baseline CNS metastases per investigator; it was 13.6 months in the subpopulation of patients with baseline CNS metastases per investigator [38]. Twenty-seven (48.2%) of the 56 patients with baseline CNS metastases per investigator experienced CNS progression, while new scan-confirmed brain lesions were discovered in 3 (2.9%) of the 105 patients without baseline CNS metastases per investigator [17].

PROs were evaluated in 145 patients with ROS1+ NSCLC participating in the STARTRK-2 study [43]. At baseline, they reported moderate to high HR-QOL and functioning (assessed using the QLQ-C30); while receiving entrectinib, they reported non-clinically meaningful improvements in HR-QOL and physical and role functioning scores that were accompanied by a non-clinically meaningful reduction in the cognitive functioning score. In general, tumour-related symptoms (assessed using the QLQ-LC13) were unchanged or improved during treatment with entrectinib, including a clinically meaningful improvement in cough [43].

Tolerability of Entrectinib

Entrectinib was generally well tolerated, based on initial [35, 36] and updated [20, 23] analyses of pooled safety data from the four ongoing phase I/II trials (ALKA-372-001 and STARTRK-1, -2 and -NG).

The integrated safety population comprised 504 patients (including 475 adults) who had received at least one dose of entrectinib; they had been exposed to the drug for a median of 5.5 months [20, 23]. The most common adverse drug reactions (ADRs) were fatigue (in 45% of patients), constipation (42.9%), dysgeusia (42.3%), dizziness (39.7%), oedema (37.3%), diarrhoea (33.5%), nausea (32.1%), dysaesthesia (29.0%), anaemia (28.2%), dyspnoea (27.0%), weight increased (26.4%), blood creatinine increased (25.4%), pain (24.4%), cognitive disorders [24.2% (29.7% and 23.1% in patients with and without baseline CNS disease, respectively)], vomiting (23.2%), cough (21.4%) and pyrexia (20.0%) [20]. Cognitive disorders included events reported as cognitive disorders (6.3%), confusional state (7.3%), memory impairment (4.2%), attention disturbance (3.8%), amnesia (2.8%), mental status changes (1.2%), hallucination (1.0%), delirium (0.8%), visual hallucination (0.4%) and mental disorder (0.2%) [20].

The most common grade ≥ 3 ADRs were anaemia (9.7%), weight increased (7.3%), lung infection (6.0%), dyspnoea (5.8%) and fatigue (5.0%); the most frequent serious ADRs were lung infection (5.2%), dyspnoea (4.6%), cognitive impairment (3.8%) and pleural effusion (2.4%) [20]. Most ADRs were transient and managed successfully with dose interruption or reduction [35, 36]; the rate of permanent entrectinib discontinuation due to ADRs was low (4.4%) [20].

The overall safety profile of entrectinib was similar in patients with NTRK+ solid tumours and those with ROS1+ NSCLC [35, 36]. Likewise, it was similar in elderly patients aged ≥ 65 years (n = 130) and younger patients aged < 65 years, albeit dizziness (48.5% vs 36.6%), blood creatinine increased (31.5% vs 23.3%), ataxia (23.8% vs 12.8%) and hypotension (21.5% vs 14.7%) occurred more frequently in the former than the latter [20].

The overall safety profile of entrectinib also appeared to be similar in paediatric patients and adults, based on updated data for 32 paediatric patients (30 enrolled in STARTRK-NG; two enrolled in STARTRK-2), including seven adolescents aged 12–17 years [20]. Grade 3 or 4 ADRs/laboratory abnormalities that occurred with an incidence ≥ 5% higher in paediatric than adult patients were neutropenia (28.1% vs 3.4%), weight increased (21.9% vs 6.9%), headache (6.3% vs 0.6%) and bone fractures (12.5% vs 1.9%) [20]. Grade ≥ 3ADRs reported in adolescents were neutropenia and headache (no further details are available) [20].

Fractures were experienced by 25 (5.3%) of the 475 adult patients and seven (21.8%) of the 32 paediatric patients [20]. Most fractures were hip or other lower extremity fractures (e.g. femoral or tibial shaft); the median time to fracture was 3.4 and 4.3 months in adults and paediatric patients, respectively. The seven paediatric patients were all aged < 12 years; they reported a total of 11 ADRs of fractures, including four grade 3 events (of which three were serious). No patient discontinued entrectinib due to fractures, and all but one event of fracture (in a paediatric patient) recovered. Some fractures in adults occurred in the setting of a fall or other trauma to the affected area, and there were also some reports of possible tumour involvement at the site of fracture. In contrast, all fractures in paediatric patients occurred in association with minimal or no trauma, and there were no reports of tumour involvement at the site of the fracture [20].

Other notable ADRs occurring in entrectinib recipients (n = 504) included peripheral sensory neuropathy (in 15.7% of patients), hyperuricaemia (in 9.1% of patients; grade ≥ 3 in 1.8%), congestive heart failure [(CHF) in 3.0% of patients; grade ≥ 3 in 2.2%], syncope (in 4.6% of patients; concurrently with hypotension, dehydration or QTc prolongation in some individuals), eye disorders [e.g. vision blurred (in 8.5% of patients), diplopia (2.6%) and visual impairment (1.6%)] and ECG QTc prolongation (in 2.0% of patients) [20]. Among patients with ≥ 1 post-baseline ECG assessment, 17 (4.0%) had a Fridericia QTc prolongation of > 60 ms; 12 (2.8%) had a Fridericia QTc of ≥ 500 ms] [20].

Dosage and Administration of Entrectinib

In the EU, entrectinib has been conditionally approved for the treatment of adult and paediatric patients aged ≥ 12 years with NTRK+ solid tumours who have a disease that is locally advanced, metastatic or where surgical resection is likely to result in severe morbidity; have not received a prior TRK inhibitor; and have no satisfactory treatment options. It has also been conditionally approved as monotherapy for the treatment of adult patients with advanced ROS1+ NSCLC not previously treated with ROS1 inhibitors [20].

Similarly, in the USA, entrectinib has been conditionally approved for the treatment of adult and paediatric patients aged ≥ 12 years with solid tumours that have a NTRK gene fusion without a known acquired resistance mechanism; are metastatic or where surgical resection is likely to result in severe morbidity; and have progressed following treatment or have no satisfactory alternative therapy. It has also been conditionally approved for the treatment of adult patients with metastatic NSCLC whose tumours are ROS1+ [21].

The recommended dosage in adults with ROS1+ NSCLC or NTRK+ solid tumours is 600 mg once daily. The recommended once-daily dosage in paediatric patients aged ≥ 12 years with NTRK+ solid tumours is based on body surface area (see local prescribing information for details) [20, 21]. Entrectinib may be taken with or without food; however, it should not be administered with grapefruit/juice (Sect. 3). It is recommended that treatment be continued until disease progression or unacceptable toxicity [20, 21].

Local prescribing information should be consulted for detailed information regarding dose modifications to manage ADRs, potential drug interactions, use in special patient populations, contraindications, and other warnings and precautions.

Place of Entrectinib in the Management of NTRK+ Solid Tumours and ROS1+ NSCLC

Entrectinib is an orally active, CNS-penetrant, small-molecule TKI of TRKA/B/C, ROS1 and ALK that has been developed to fulfill unmet clinical needs, including for a targeted therapy in NTRK+ solid tumours and ROS1+ NSCLC that has activity against brain tumours (either primary or metastatic) in addition to systemic efficacy (Sect. 1).

The efficacy of entrectinib monotherapy in treating a variety of locally-advanced or metastatic NTRK+ solid tumours in TRK inhibitor-naïve adults has been established according to an integrated analysis of three ongoing, single-arm trials, in which the drug induced clinically meaningful and durable systemic responses independent of the tumour type as well as the NTRK fusion type, and irrespective of the presence or absence of CNS metastases at baseline (Sect. 4.1). Moreover, in patients with measurable CNS metastases at baseline, the IC efficacy of entrectinib was substantial and comparable to its systemic efficacy (Sect. 4.1). The efficacy of entrectinib in paediatric patients aged ≥ 12 years with NTRK+ solid tumours was established, based on extrapolation of data from the single-arm studies in adults and relevant data from children and adolescents with recurrent or refractory NTRK+ CNS/solid tumours enrolled in another single-arm study (Sect. 4.1.1). Patients aged < 21 years with recurrent or refractory solid tumours (including primary CNS tumours) harbouring NTRK or ROS1 fusions are continuing to be enrolled in the expansion cohorts of this ongoing trial [45].

Due to its demonstrable activity against solid tumours harbouring a specific molecular alteration (in this case an NTRK fusion), regardless of their location, entrectinib is referred to as a ‘tumour-agnostic’ or ‘histology-independent’ therapy. It is the second such tumour-agnostic therapy to be conditionally approved for the treatment of locally-advanced or metastatic NTRK+ solid tumours, after larotrectinib (also on the basis of an integrated analysis of efficacy in three single-arm trials [46]). In terms of the EU-approved indication, the main difference between the two TKIs is that entrectinib is indicated for adults and paediatric patients aged ≥ 12 years [20], whereas larotrectinib is indicated for adults and paediatric patients of all ages [46]. Additionally, the EU label for entrectinib [20], unlike that for larotrectinib [46], specifies that patients must not have received a prior TRK inhibitor (Sect. 6). The conditional approval status of entrectinib and larotrectinib in the EU means that further evidence on these medicinal products is awaited; the European Medicines Agency will review new information at least annually and update the SPC as necessary [20, 46].

Entrectinib, like larotrectinib, has been incorporated into National Comprehensive Cancer Network (NCCN) guidelines pertaining to the treatment of various advanced or metastatic NTRK+ solid tumours [47] (Table 4). For example, both TKIs are recommended as a preferred option for the first-line treatment of patients with advanced or metastatic NTRK+ NSCLC; they are also recommended as an option for subsequent therapy in these patients following progression on alternative first-line systemic therapies that may be useful in certain circumstances (e.g. albumin-bound paclitaxel, docetaxel and gemcitabine) [47] (Table 4). With respect to tumours in the CNS, both entrectinib and larotrectinb are recommended as a systemic therapy for NTRK+ solid tumours with brain metastases [47] (Table 4). In this regard, entrectinib has demonstrable IC efficacy in adults with NTRK+ solid tumours and brain metastases (Sect. 4.1). Furthermore, there are data (albeit with limitations and therefore requiring cautious interpretation) suggestive of a potential CNS-protective effect of entrectinib in terms of preventing the onset of new lesions in patients without baseline CNS metastases (Sect. 4.1). Larotrectinib has also reportedly shown IC activity, including in a small number of patients with NTRK+ solid tumours and measurable brain metastases at baseline [48]. However, due to the rarity of NTRK+ solid tumours, directly comparing the two TKIs in randomized trials (e.g. focusing on IC activity in patients with metastatic brain tumours) is not considered feasible. Moreover, any indirect comparison of the two TKIs based on their existing integrated analyses of efficacy will be subject to a number of limitations. These include reliance on data derived from single-arm trials, low patient numbers and heterogeneous study populations, notably with respect to age (i.e. the larotectinib, but not entrectinib, cohort includes both adults and paediatric patients), the proportions of patients with primary/metastatic brain tumours (e.g. the entrectinib cohort currently has a higher proportion of patients with CNS metastases than the larotrectinib cohort [20, 46, 49]), the types and relative proportions of the NTRK+ solid tumours represented and the number and type of prior lines of therapy. Further expansion of the integrated analyses in addition to the emergence of real-world data from routine clinical practice will increase the evidence base for, and hence the robustness of, indirect comparisons of the relative efficacy of these two agents [7]; hence, both developments are awaited with interest. On-target resistant mutations have been identified in patients who have progressed on entrectinib (Sect. 2.1), as well as larotrectinib, indicating that these represent a shared liability for these first-generation TRK inhibitors [49]. Second-generation TRK inhibitors designed to overcome acquired on-target resistance to first-generation agents are currently being evaluated [49, 50].

Table 4

Recommendations regarding entrectinib as a treatment option in patients with NTRK fusion-positive solid tumours: summary of National Comprehensive Cancer Network (NCCN) guidelines [47]

Tumour type	Recommendationa
Breast cancer	Option in recurrent/stage IV disease in pts with no satisfactory alternatives or who have progressed after treatment
Cervical cancer	Option for 2nd-line treatment of recurrent/metastatic disease
Colon/rectal cancer	Subsequent-line option for metastatic disease following initial therapy
Cutaneous melanoma	Option for 2nd-line or subsequent-line treatment of metastatic or unresectable disease
CNS cancers	Option for tumours with newly-diagnosed or recurrent brain metastases
Gastric cancer	Other recommended regimens option for 2nd-line or subsequent-line treatment of unresectable locally advanced, recurrent, or metastatic disease, where local therapy is not indicated
Head/neck cancers	Option for treatment of recurrent salivary gland tumours with distant metastases in pts with a PS of 0–3
Hepatobiliary cancers	Subsequent-line option for HCC that is: unresectable and pt is not a transplant candidate or inoperable by PS or comorbidity, local/local with minimal extrahepatic disease only or metastatic or has extensive liver tumour burden Option for primary treatment and subsequent-line therapy upon disease progression of unresectable or metastatic gallbladder cancer, ICC or ECC
NSCLC	Preferred option for 1st-line and option for subsequent-line treatment of pts with advanced or metastatic disease
Oesophageal cancer/EJCs	Other recommended regimens option for 2nd-line or subsequent-line treatment of unresectable locally advanced, recurrent, or metastatic oesophageal cancer or EJCs, where local therapy is not indicated
Ovarian cancer	Option as recurrence therapy for platinum-sensitive and -resistant disease in pts with EOC, FTC, PPC, including LCOH
Pancreatic adenocarcinoma	1st-line option for metastatic disease in pts with poor PS; 2nd-line option for locally advanced/metastatic disease or therapy for recurrent disease in pts with good or poor PS
STS	Option for STS subtypes with non-specific histologies (not intended for adjuvant therapy of non-metastatic disease)
Thyroid cancer	Option for treatment of advanced, progressive, or threatening papillary carcinoma, follicular carcinoma, or Hurthle cell carcinoma; preferred regimen for treatment of metastatic (stage IVC) anaplastic carcinoma
Vulvar cancer	Option for treatment of advanced, recurrent or metastatic disease

ECC extrahepatic cholangiocarcinoma, EJCs esophagogastric junction cancers, EOC epithelial ovarian cancer, FTC fallopian tube cancer, HCC hepatocellular carcinoma, ICC intrahepatic cholangiocarcinoma, LCOH less common ovarian cancers, NSCLC non-small cell lung cancer, PPC primary peritoneal cancer, pt(s) patient(s), PS performance status, STS soft tissue sarcoma

aAlso applies to larotrectinib

The efficacy of entrectinib monotherapy in treating in ROS1+ NSCLC in ROS1 inhibitor-naïve adults has also been established according to the integrated analysis of the three ongoing, single-arm trials, in which the drug induced clinically meaningful and durable systemic responses, both in patients with and without baseline CNS metastases (Sect. 4.2). Entrectinib showed marked IC antitumour activity in patients with baseline CNS metastases and, as for patients with NTRK+ solid tumours, there are data (albeit with limitations and hence requiring cautious interpretation) suggestive of a potential CNS-protective effect of entrectinib in patients without baseline CNS metastases (Sect. 4.2).

Importantly, the efficient brain penetration and potent IC activity of entrectinib represents a potential advantage over crizotinib, the current standard of care for ROS1+ NSCLC, which demonstrates poor brain penetration and, hence, limited CNS activity [51] (Sect. 1). Accordingly, as set out in the NCCN guideline for ROS1+ NSCLC [47], entrectinib is a preferred first-line therapy alongside crizotinib, albeit with a note that it may be better for patients with brain metastases.

A head-to-head study directly comparing entrectinib with crizotinib in patients with advanced ROS1+ NSCLC, with or without CNS metastases, has commenced (NCT04603807). This randomized, open-label, multicentre, phase III study will enrol an estimated 220 patients and has an estimated completion date of late 2027; the primary endpoint is PFS in participants with baseline CNS metastases. Currently, the relative benefits of these two TKIs have been comprehensively assessed using the matching-adjusted indirect comparisons method [52]. Briefly, data for entrectinib were derived from the initial integrated analysis of the ALKA-372-001, STARTRK-1 and STARTRK-2 trials, while that for crizotinib were derived from the pivotal PROFILE 1001 study. Matching was based on known prognostic/predictive factors and, notably, the proportion of patients with CNS metastases (not reported in PROFILE 1001) was varied across three scenarios. The results of this analysis suggested improved outcomes with entrectinib versus crizotinib, with the former yielding significantly better ORRs than the latter in all three scenarios. OS and treatment discontinuation due to adverse events also favoured entrectinib, while PFS was generally similar [52]. Another analysis that indirectly compared the entrectinib clinical trial population (initial integrated analysis) with a matched real-world crizotinib population also suggested improved outcomes with the former versus the latter in terms of a longer PFS and a longer time to treatment discontinuation [53]. Accordingly, data on the effectiveness of entrectinib in ROS1+ NSCLC in clinical practice are awaited with interest, as are comparisons of the drug with crizotinib, based on real-world cohorts of ROS1+ NSCLC patients.

The NCCN guideline for ROS1+ NSCLC [47] also recommends entrectinib as an option for subsequent therapy, albeit with a note that it is primarily for patients with CNS progression after crizotinib [47]. The extent of the role of entrectinib in patients who develop systemic disease progression due to crizotinib resistance is uncertain [54], primarily because it does not show activity against the most common acquired mutation that confers resistance to crizotinib in ROS1+ NSCLC, namely ROS1-G2032R [15] (Sect. 2.1). The administration of entrectinib to ROS1+ NSCLC patients with acquired resistance to crizotinib is not supported by the limited amount of relevant data currently available [54]; further reports of experience with the drug in this setting would be of considerable interest. Most crizotinib-treated patients eventually progress, either as a result of acquired resistance or, frequently, the development of CNS metastases; this, in view of the CNS activity of entrectinib and its ineffectiveness against ROS1-G2032R, argues in favour of using entrectinib before, rather than after, crizotinib in advanced ROS1+ NSCLC, particularly in patients with baseline brain metastases [17, 54]. ROS1 TKIs with broad activity against kinase mutations, including ROS1-G2032R, are currently being evaluated [50, 54].

Of note, PROs from patients with NTRK+ solid tumours or ROS1+ NSCLC enrolled in STARTRK-2 have been presented in preliminary form (Sect. 4). The results support a positive benefit-risk profile for entrectinib; importantly, the drug is associated with a minimal treatment-related symptom burden [42, 43].

Entrectinib was generally well tolerated, with a manageable safety profile that was similar in patients with NTRK+ solid tumours and those with ROS1+ NSCLC, as well as in paediatric patients, younger adults and the elderly, based on data pooled from two or all four of the ongoing, single arm trials (Sect. 5). Among the most commonly reported ADRs, dizziness, weight increased and cognitive disorders are potentially related to TRK inhibition; hence, their occurrence is not unexpected [36]. Local prescribing information (e.g. the EU SPC [20]) should be consulted for recommended entrectinib dose modifications (interruption, reduction or discontinuation) to manage ADRs, such as cognitive changes, hyperuricaemia, QT interval prolongation and CHF (Sect. 6). According to the EU SPC [20], entrectinib should be avoided in patients with a baseline corrected QT interval (QTc) > 450 ms, those with congenital long QTc syndrome, and those taking medicinal products known to prolong the QTc interval [20]. Entrectinib should also be avoided in patients with electrolyte imbalances or significant cardiac disease, including recent myocardial infarction, CHF, unstable angina and bradyarrhythmias. Additional monitoring should be performed (e.g. assessment of left ventricular ejection fraction in patients with symptoms or known risk factors of CHF) and a specialist consultation considered, if the drug is to be prescribed to patients with any of these conditions. Assessment of ECG and electrolytes at baseline, 1 month after starting entrectinib and periodically throughout treatment (as clinically indicated) is recommended [20].

The assays currently required to select patients with NTRK+ solid tumours or ROS1+ NSCLC who are eligible to receive entrectinib are typically time-consuming and rely on invasive tissue biopsies [55]. Hence, a non-invasive (in vitro), next-generation sequencing-based companion diagnostic [FoundationOne® Liquid CDx (F1L®CDx)] is under development [56]. Recently, the clinical validity of the F1L®CDx assay as a complementary aid to tissue-based testing for the identification of patients with NTRK+ solid tumours and ROS1+ NSCLC who may benefit from entrectinib treatment was demonstrated in a bridging study [55].

In conclusion, entrectinib expands the range of treatment options for advanced NTRK+ solid tumours and ROS1+ NSCLC, and may be of particular value in patients with existing CNS metastases and those who are at risk of developing CNS metastases.

Data Selection Entrectinib: 293 records identified

Below is the link to the electronic supplementary material.

Supplementary material 2(PPTX 18604 kb)

Supplementary material 3(PDF 154 kb)

Digital Features for this Adis Drug Evaluation can be found at 10.6084/m9.figshare.14150207

Oral inhibitor of TRKA/B/C, ROS1 and ALK tyrosine kinases

Designed to be active systemically and in the CNS

Demonstrated durable systemic and intracranial efficacy

Generally well tolerated with a manageable safety profile

Duplicates removed	65
Excluded during initial screening (e.g. press releases; news reports; not relevant drug/indication; preclinical study; reviews; case reports; not randomized trial)	127
Excluded during writing (e.g. reviews; duplicate data; small patient number; nonrandomized/phase I/II trials)	45
Cited efficacy/tolerability articles	11
Cited articles not efficacy/tolerability	45
Search Strategy: EMBASE, MEDLINE and PubMed from 1946 to present. Clinical trial registries/databases and websites were also searched for relevant data. Key words were entrectinib, Rozlytrek, NSCLC, solid tumours. Records were limited to those in English language. Searches last updated 25 March 2021.