Literature DB >> 33847987

Targeted Integration Through Transformation of Hydroxyurea-Arrested Cells.

Vasiliki Tsakraklides1.   

Abstract

Homologous recombination is required to specifically target DNA to a desired genomic locus. Non-homologous end joining is the predominant form of recombination in Yarrowia lipolytica. Transformation of this organism with linear DNA therefore results in random integration of the introduced DNA into the genome. In this protocol, hydroxyurea-mediated cell cycle arrest is applied to significantly increase the rate of homologous recombination during transformation and enhance targeted integration.

Entities:  

Keywords:  Deletion; Homologous recombination; Hydroxyurea; Knock-out; Targeted integration; Yarrowia lipolytica

Year:  2021        PMID： 33847987     DOI： 10.1007/978-1-0716-1414-3_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Targeted gene replacement in fungi using a split-marker approach.

Authors:  Rubella S Goswami
Journal:  Methods Mol Biol       Date:  2012

Review 2.  Playing the end game: DNA double-strand break repair pathway choice.

Authors:  J Ross Chapman; Martin R G Taylor; Simon J Boulton
Journal:  Mol Cell       Date:  2012-08-24       Impact factor: 17.970

Review 3.  Cell cycle regulation of homologous recombination in Saccharomyces cerevisiae.

Authors:  David P Mathiasen; Michael Lisby
Journal:  FEMS Microbiol Rev       Date:  2014-02-24       Impact factor: 16.408

4.  Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration.

Authors:  H van Attikum; P Bundock; P J Hooykaas
Journal:  EMBO J       Date:  2001-11-15       Impact factor: 11.598

5.  Quantitative live cell imaging reveals a gradual shift between DNA repair mechanisms and a maximal use of HR in mid S phase.

Authors:  Ketki Karanam; Ran Kafri; Alexander Loewer; Galit Lahav
Journal:  Mol Cell       Date:  2012-07-27       Impact factor: 17.970

6.  Improved Gene Targeting through Cell Cycle Synchronization.

Authors:  Vasiliki Tsakraklides; Elena Brevnova; Gregory Stephanopoulos; A Joe Shaw
Journal:  PLoS One       Date:  2015-07-20       Impact factor: 3.240

7.  High-oleate yeast oil without polyunsaturated fatty acids.

Authors:  Vasiliki Tsakraklides; Annapurna Kamineni; Andrew L Consiglio; Kyle MacEwen; Jonathan Friedlander; Hannah G Blitzblau; Maureen A Hamilton; Donald V Crabtree; Austin Su; Jonathan Afshar; John E Sullivan; W Greg LaTouf; Colin R South; Emily H Greenhagen; A Joe Shaw; Elena E Brevnova
Journal:  Biotechnol Biofuels       Date:  2018-05-09       Impact factor: 6.040
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.