Literature DB >> 33847962

Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-Stranded DNA-Binding Proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A).

Serene El-Kamand1, Mar-Dean Du Plessis1, Teegan Lawson1, Liza Cubeddu2,3, Roland Gamsjaeger4,5.   

Abstract

Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years, we have used NMR spectroscopy to determine the molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.

Entities:  

Keywords:  Cancer; DNA repair; NMR; hSSB1; hSSB2

Year:  2021        PMID： 33847962     DOI： 10.1007/978-1-0716-1290-3_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

Review 1.  Macromolecular NMR spectroscopy for the non-spectroscopist.

Authors:  Ann H Kwan; Mehdi Mobli; Paul R Gooley; Glenn F King; Joel P Mackay
Journal:  FEBS J       Date:  2011-01-28       Impact factor: 5.542

Review 2.  Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.

Authors:  Michael Bieri; Ann H Kwan; Mehdi Mobli; Glenn F King; Joel P Mackay; Paul R Gooley
Journal:  FEBS J       Date:  2011-01-28       Impact factor: 5.542

Review 3.  The structural analysis of protein-protein interactions by NMR spectroscopy.

Authors:  Mitchell R O'Connell; Roland Gamsjaeger; Joel P Mackay
Journal:  Proteomics       Date:  2009-12       Impact factor: 3.984

4.  NMR studies on protein-nucleic acid interaction.

Authors:  Robert Kaptein
Journal:  J Biomol NMR       Date:  2013-05       Impact factor: 2.835

5.  The structural details of the interaction of single-stranded DNA binding protein hSSB2 (NABP1/OBFC2A) with UV-damaged DNA.

Authors:  Teegan Lawson; Serene El-Kamand; Didier Boucher; Duc Cong Duong; Ruvini Kariawasam; Alexandre M J J Bonvin; Derek J Richard; Roland Gamsjaeger; Liza Cubeddu
Journal:  Proteins       Date:  2019-08-30

Review 6.  Human single-stranded DNA binding protein 1 (hSSB1, OBFC2B), a critical component of the DNA damage response.

Authors:  Laura V Croft; Emma Bolderson; Mark N Adams; Serene El-Kamand; Ruvini Kariawasam; Liza Cubeddu; Roland Gamsjaeger; Derek J Richard
Journal:  Semin Cell Dev Biol       Date:  2018-03-28       Impact factor: 7.727

Review 7.  Single-stranded DNA-binding proteins: multiple domains for multiple functions.

Authors:  Thayne H Dickey; Sarah E Altschuler; Deborah S Wuttke
Journal:  Structure       Date:  2013-07-02       Impact factor: 5.006

8.  Backbone 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB2 (NABP1/OBFC2A) and chemical shift mapping of the DNA-binding interface.

Authors:  Ruvini Kariawasam; Maddison Knight; Roland Gamsjaeger; Liza Cubeddu
Journal:  Biomol NMR Assign       Date:  2017-10-24       Impact factor: 0.746

9.  Backbone (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB1 (NABP2/OBFC2B) and chemical shift mapping of the DNA-binding interface.

Authors:  Ruvini Kariawasam; Christine Touma; Liza Cubeddu; Roland Gamsjaeger
Journal:  Biomol NMR Assign       Date:  2016-05-18       Impact factor: 0.746

Review 10.  A Structural Perspective on the Regulation of Human Single-Stranded DNA Binding Protein 1 (hSSB1, OBFC2B) Function in DNA Repair.

Authors:  Teegan Lawson; Serene El-Kamand; Ruvini Kariawasam; Derek J Richard; Liza Cubeddu; Roland Gamsjaeger
Journal:  Comput Struct Biotechnol J       Date:  2019-03-28       Impact factor: 7.271
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