Literature DB >> 33847956

Single-Molecule Tethered Particle Motion Studies on the DNA Recombinase Filament Assembly and Disassembly.

Chih-Hao Lu1,2, Wei-Hsuan Lan1, Hung-Wen Li3.   

Abstract

Bacterial RecA and eukaryotic Rad51 are recombinases indispensable for DNA homologous recombination and repair of double-stranded DNA breaks. Understanding the functions and biophysical properties of the DNA recombinases benefits the research in human medicine such as cancer biology. Single-molecule techniques provide the mechanistic details of complex biological reactions. Tethered particle motion (TPM) experiment is a simple and multiplex single-molecule tool to monitor DNA-protein interactions. We have developed a single-molecule TPM assay to study DNA recombinase filament assembly and disassembly on individual DNA molecules in real time. Characterization of the temporal change of the Brownian motion of DNA tethers during recombinase assembly and disassembly in real time allows the determination of multiple kinetic parameters of nucleation rate, extension rate, dissociation rate, and length of the recombinase-DNA filament.

Entities:  

Keywords:  DNA homologous recombination; Rad51; RecA; SSB; Single-molecule microscopy; Tethered particle motion

Year:  2021        PMID： 33847956     DOI： 10.1007/978-1-0716-1290-3_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  44 in total

Review 1.  Motoring along with the bacterial RecA protein.

Authors:  Michael M Cox
Journal:  Nat Rev Mol Cell Biol       Date:  2007-01-17       Impact factor: 94.444

Review 2.  Mechanism of eukaryotic homologous recombination.

Authors:  Joseph San Filippo; Patrick Sung; Hannah Klein
Journal:  Annu Rev Biochem       Date:  2008       Impact factor: 23.643

3.  Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family.

Authors:  S Keeney; C N Giroux; N Kleckner
Journal:  Cell       Date:  1997-02-07       Impact factor: 41.582

Review 4.  Mechanism of homologous recombination: mediators and helicases take on regulatory functions.

Authors:  Patrick Sung; Hannah Klein
Journal:  Nat Rev Mol Cell Biol       Date:  2006-08-23       Impact factor: 94.444

5.  Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death.

Authors:  E Sonoda; M S Sasaki; J M Buerstedde; O Bezzubova; A Shinohara; H Ogawa; M Takata; Y Yamaguchi-Iwai; S Takeda
Journal:  EMBO J       Date:  1998-01-15       Impact factor: 11.598

Review 6.  Regulation of homologous recombination in eukaryotes.

Authors:  Wolf-Dietrich Heyer; Kirk T Ehmsen; Jie Liu
Journal:  Annu Rev Genet       Date:  2010       Impact factor: 16.830

Review 7.  Regulation of bacterial RecA protein function.

Authors:  Michael M Cox
Journal:  Crit Rev Biochem Mol Biol       Date:  2007 Jan-Feb       Impact factor: 8.250

Review 8.  The bacterial RecA protein as a motor protein.

Authors:  Michael M Cox
Journal:  Annu Rev Microbiol       Date:  2003       Impact factor: 15.500

9.  SSB protein diffusion on single-stranded DNA stimulates RecA filament formation.

Authors:  Rahul Roy; Alexander G Kozlov; Timothy M Lohman; Taekjip Ha
Journal:  Nature       Date:  2009-10-11       Impact factor: 49.962

10.  Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA.

Authors:  Jason C Bell; Jody L Plank; Christopher C Dombrowski; Stephen C Kowalczykowski
Journal:  Nature       Date:  2012-10-24       Impact factor: 49.962
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