Literature DB >> 9430650

Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death.

E Sonoda1, M S Sasaki, J M Buerstedde, O Bezzubova, A Shinohara, H Ogawa, M Takata, Y Yamaguchi-Iwai, S Takeda.   

Abstract

Yeast rad51 mutants are viable, but extremely sensitive to gamma-rays due to defective repair of double-strand breaks. In contrast, disruption of the murine RAD51 homologue is lethal, indicating an essential role of Rad51 in vertebrate cells. We generated clones of the chicken B lymphocyte line DT40 carrying a human RAD51 transgene under the control of a repressible promoter and subsequently disrupted the endogenous RAD51 loci. Upon inhibition of the RAD51 transgene, Rad51- cells accumulated in the G2/M phase of the cell cycle before dying. Chromosome analysis revealed that most metaphase-arrested Rad51- cells carried isochromatid-type breaks. In conclusion, Rad51 fulfils an essential role in the repair of spontaneously occurring chromosome breaks in proliferating cells of higher eukaryotes.

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Year:  1998        PMID： 9430650      PMCID： PMC1170409          DOI： 10.1093/emboj/17.2.598

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  74 in total

1.  The immunology and developmental biology of the chicken.

Authors:  O Vainio; B A Imhof
Journal:  Immunol Today       Date:  1995-08

2.  Sensitivity and selectivity of the DNA damage sensor responsible for activating p53-dependent G1 arrest.

Authors:  L C Huang; K C Clarkin; G M Wahl
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

3.  Cell cycle-dependent expression of the mouse Rad51 gene in proliferating cells.

Authors:  A Yamamoto; T Taki; H Yagi; T Habu; K Yoshida; Y Yoshimura; K Yamamoto; A Matsushiro; Y Nishimune; T Morita
Journal:  Mol Gen Genet       Date:  1996-04-24

4.  A human RNA polymerase II complex associated with SRB and DNA-repair proteins.

Authors:  E Maldonado; R Shiekhattar; M Sheldon; H Cho; R Drapkin; P Rickert; E Lees; C W Anderson; S Linn; D Reinberg
Journal:  Nature       Date:  1996-05-02       Impact factor: 49.962

Review 5.  Homologous recombination and the roles of double-strand breaks.

Authors:  A Shinohara; T Ogawa
Journal:  Trends Biochem Sci       Date:  1995-10       Impact factor: 13.807

6.  S phase specific formation of the human Rad51 protein nuclear foci in lymphocytes.

Authors:  S Tashiro; N Kotomura; A Shinohara; K Tanaka; K Ueda; N Kamada
Journal:  Oncogene       Date:  1996-05-16       Impact factor: 9.867

7.  Replication slippage between distant short repeats in Saccharomyces cerevisiae depends on the direction of replication and the RAD50 and RAD52 genes.

Authors:  H T Tran; N P Degtyareva; N N Koloteva; A Sugino; H Masumoto; D A Gordenin; M A Resnick
Journal:  Mol Cell Biol       Date:  1995-10       Impact factor: 4.272

8.  Membrane-associated CD19-LYN complex is an endogenous p53-independent and Bc1-2-independent regulator of apoptosis in human B-lineage lymphoma cells.

Authors:  D E Myers; X Jun; K G Waddick; C Forsyth; L M Chelstrom; R L Gunther; N E Tumer; J Bolen; F M Uckun
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-10       Impact factor: 11.205

9.  p53 is linked directly to homologous recombination processes via RAD51/RecA protein interaction.

Authors:  H W Stürzbecher; B Donzelmann; W Henning; U Knippschild; S Buchhop
Journal:  EMBO J       Date:  1996-04-15       Impact factor: 11.598

10.  Requirement of phospholipase C-gamma 2 activation in surface immunoglobulin M-induced B cell apoptosis.

Authors:  M Takata; Y Homma; T Kurosaki
Journal:  J Exp Med       Date:  1995-10-01       Impact factor: 14.307
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  284 in total

1.  Impairment of lagging strand synthesis triggers the formation of a RuvABC substrate at replication forks.

Authors:  M J Flores; H Bierne; S D Ehrlich; B Michel
Journal:  EMBO J       Date:  2001-02-01       Impact factor: 11.598

2.  Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells.

Authors:  E Sonoda; M S Sasaki; C Morrison; Y Yamaguchi-Iwai; M Takata; S Takeda
Journal:  Mol Cell Biol       Date:  1999-07       Impact factor: 4.272

3.  Characterization of mammalian RAD51 double strand break repair using non-lethal dominant-negative forms.

Authors:  S Lambert; B S Lopez
Journal:  EMBO J       Date:  2000-06-15       Impact factor: 11.598

4.  Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of holliday junction resolution.

Authors:  A J van Gool; N M Hajibagheri; A Stasiak; S C West
Journal:  Genes Dev       Date:  1999-07-15       Impact factor: 11.361

5.  A cell cycle-specific requirement for the XRCC1 BRCT II domain during mammalian DNA strand break repair.

Authors:  R M Taylor; D J Moore; J Whitehouse; P Johnson; K W Caldecott
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

Review 6.  Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40.

Authors:  E Sonoda; C Morrison; Y M Yamashita; M Takata; S Takeda
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2001-01-29       Impact factor: 6.237

7.  Role for caspase-mediated cleavage of Rad51 in induction of apoptosis by DNA damage.

Authors:  Y Huang; S Nakada; T Ishiko; T Utsugisawa; R Datta; S Kharbanda; K Yoshida; R V Talanian; R Weichselbaum; D Kufe; Z M Yuan
Journal:  Mol Cell Biol       Date:  1999-04       Impact factor: 4.272

8.  Sister chromatid gene conversion is a prominent double-strand break repair pathway in mammalian cells.

Authors:  R D Johnson; M Jasin
Journal:  EMBO J       Date:  2000-07-03       Impact factor: 11.598

9.  Activation of Cdh1-dependent APC is required for G1 cell cycle arrest and DNA damage-induced G2 checkpoint in vertebrate cells.

Authors:  T Sudo; Y Ota; S Kotani; M Nakao; Y Takami; S Takeda; H Saya
Journal:  EMBO J       Date:  2001-11-15       Impact factor: 11.598

Review 10.  Homologous DNA recombination in vertebrate cells.

Authors:  E Sonoda; M Takata; Y M Yamashita; C Morrison; S Takeda
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205
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