Morphological Changes in Cortical and Subcortical Structures in Multiple System Atrophy Patients With Mild Cognitive Impairment.

OBJECTIVE: This study aimed to investigate the morphometric alterations in the cortical and subcortical structures in multiple system atrophy (MSA) patients with mild cognitive impairment (MCI), and to explore the association with cognitive deficits.
METHODS: A total of 45 MSA patients (25 MSA-only, 20 MSA-MCI) and 29 healthy controls were recruited. FreeSurfer software was used to analyze cortical thickness, and voxel-based morphometry was used to analyze the gray matter volumes. Cortical thickness and gray matter volume changes were correlated with cognitive scores.
RESULTS: Compared to healthy controls, both MSA subgroups exhibited widespread morphology alterations of brain structures in the fronto-temporal regions. Direct comparison of MSA-MCI and MSA-only patients showed volume reduction in the left superior and middle temporal gyrus, while cortical thinning was found in the left middle and inferior temporal gyrus in MSA-MCI patients. Cortical thinning in the left middle temporal gyrus correlated with cognitive assessment and disease duration.
CONCLUSION: Structural changes in the brain occur in MSA-MCI patients. The alteration of brain structure in the left temporal regions might be a biomarker of cognitive decline in MSA-MCI patients.

Introduction

Multiple system atrophy (MSA) is a progressive neuro-degenerative disease with a wide range of motor and non-motor symptoms. Current research, assessments, and interventions mainly focus on motor impairment in patients with MSA, such as gait ataxia, limb ataxia, and scanning dysarthria (Iodice et al., 2012). However, relatively little research has been done on non-motor symptoms. As a domain that cannot be ignored in non-motor symptoms, cognitive impairment (CI) affects the processing speed and executive functions of MSA patients, and brings great burden to patients and their families (Koga et al., 2017). Unfortunately, the neuropathological mechanisms of cognitive impairment in MSA patients remain unclear, and this uncertainty might limit further research on MSA patients with cognitive deficits.

Structural magnetic resonance imaging (MRI) is a powerful tool for the study of neuro-degenerative disease. Previous structural MRI studies mainly used voxel-based morphometry (VBM) measures to investigate gray matter (GM) abnormalities. Significant subcortical structural impairment was found in MSA patients with CI (Lee et al., 2016). Further studies found that GM atrophy in the bilateral thalamus, the left cerebellum, and the left pericalcarine gyrus was significantly associated with cognitive deficits in the cerebellar subtype of MSA (Lee et al., 2016); In the parkinsonian subtype of MSA, cerebellum, striatal, temporal, and frontal cortical areas of GM atrophy showed correlation with cognitive deficits (Kim et al., 2015; Caso et al., 2019). These results illustrated that cognitive dysfunction was associated with widespread fronto-temporal subcortical GM, basal ganglia, and cerebellum atrophies in MSA patients (Fiorenzato et al., 2017). These studies focused on subcortical and infratentorial structural alterations in MSA patients, while little attention was paid to the changes of the cortex. Primary cortical injury and subcortical structural degeneration were reported to co-exist in MSA patients (Kim et al., 2013). Previous studies confirmed that the injury of motor cortex in MSA patients contributed to motor dysfunction (Su et al., 2001), but unfortunately studies aimed at the changes of cortical morphology in MSA patients with mild cognitive impairment (MCI) were lacking.

FreeSurfer is a software suite utilizing surface-based morphological analysis that provides accurate information on cortical thickness and is considered the gold standard for measuring cortical thickness (Seiger et al., 2018). Colloby et al. (2019) used FreeSurfer to explore dementia with Lewy bodies and Alzheimer’s disease (AD) dementia and found that, compared to AD, dementia with Lewy bodies retained the cortical structure of the medial temporal lobule, which could be a biomarker for dementia with Lewy bodies. Hanford et al. (2019), in a FreeSurfer study of schizophrenia, found a significant relationship between the cognitive impairment and the cortical thickness of the prefrontal cortex. These studies demonstrate the great potential of FreeSurfer in cortical studies in MSA-MCI patients.

The purpose of our study was to use FreeSurfer analysis combined with VBM to investigate the brain morphological changes that might cause cognitive decline in MSA-MCI patients. The results might help to better understand the relationship between CI and brain structure in MSA patients. We hypothesized that the changes in brain structure, particularly in the fronto-temporal lobules, were associated with the cognitive decline in MSA-MCI patients.

Materials and Methods

Subjects

Forty-five patients [25 MSA-no cognitive impairment (NCI) and 20 MSA-MCI patients] from the neurology outpatient clinic at the First Affiliated Hospital of China Medical University were enroled. Patients were diagnosed by a movement disorder specialist with “probable MSA” according to the MSA diagnostic criteria (second edition, 2008) (Gilman et al., 2008). Further, we recruited 29 age-, gender-, and education-matched healthy controls (HC). This study was approved by the ethics review committee of the China Medical University. Patient’s informed consent was obtained before the experiment. All participants had no mental or depressive symptoms. All participants were right-handed, Han Chinese, and had no contraindications to MRI. Subjects were excluded if they had: (i) symptomatic onset before 30 years of age, (ii) substance abuse that could cause cognitive impairment, or (iii) history of neurological illness.

Clinical and Neuropsychological Assessment

Cognitive status was assessed using the Montreal Cognitive Assessment (MoCA). We used the Movement Disorders Society Task Force criteria to assess MCI (Goldman et al., 2014). For the level I criteria, we defined cognitive impairment as an MoCA score < 26. For the level II criteria, we used 1.5 standard deviations (SD) below the normative values (healthy controls) as a cutoff. Patients were excluded if they fulfiled the Task Force criteria for Parkinson’s disease dementia: (i) MMSE score ≤ 25, (ii) cognitive deficiency severe enough to impair daily life, and (iii) impairment in more than one cognitive domain. Healthy controls with an MoCA score < 26, or < 25 for secondary school-educated subjects (12 years of education), were excluded. Additionally, parts II and III of the Unified Multiple System Atrophy Rating Scale (UMSARS) were used to rate the disease severity.

MRI Acquisition

MRIs were acquired on a 3.0T MRI scanner (Magnetom Verio, Siemens Healthineers, Germany) equipped with a 32-channel head coil located at the First Affiliated Hospital of China Medical University. High-resolution three-dimensional sagittal T1-weighted images were acquired in a magnetization-prepared rapid acquisition gradient-echo sequence with the following parameters: TR, 5,000 ms; TE, 2,960 ms; FOV, 256 × 256 mm2; flip angle, 12°; dist. Factor, 0.5; matrix size, 256 × 256; slice thickness, 1 mm; voxel size, 1.0 × 1.0 × 1.0 mm; slice number, 176.

Imaging Data Preprocessing

Cortical Thickness Measurement

FreeSurfer image analysis suite, version 6.0 (Fischl and Dale, 2000)[1], was used for cortical reconstruction and thickness analysis on the three-dimensional T1-weighted images data. Details of the methodological have been described previously (Fischl et al., 1999). In brief, the analysis pipeline included non-uniform intensity correction; Talairach transformation; removal of non-brain tissue; segmentation of the images into GM, white matter (WM), and cerebrospinal fluid (CSF); and tessellation of WM/GM boundary. The surface was then wrapped following intensity gradients to optimally identify the WM/GM and GM/CSF (pial) borders. In this segmentation procedure, we can manually edit by adding control points if necessary. Surface inflation, spherical atlas registration, and the parcelation of the cortex into gyral and sulcal structures were performed. Cortical thickness was then defined as the shortest distance from WM/GM boundary to pial surface at each vertex. Finally, the surface maps were realigned to a common surface space and smoothed with a 10 mm full width at half maximum surface-based Gaussian kernel.

Whole-Brain VBM Analysis

VBM was performed using SPM12 software package (Ashburner and Friston, 2000)[2]. The Diffeomorphic Anatomical Registration Through Exponentiated Lie Algebra (DARTEL) registration method (Ashburner, 2007) was used to analyze whole-brain GM abnormalities. All three-dimensional T1-weighted images were segmented into GM, WM, and CSF in MNI space. The study-specific template was created by DARTEL procedure, and the initial registration of the template into the tissue probability map was performed to create wrapped images. The Jacobian determinant was used for modulation to guarantee that the relative volume of GM was retained after the spatial normalization. The resulting images were smoothed with an 8 mm full width at half maximum Gaussian kernel.

Statistical Analysis

SPSS 20.0 software (IBM Software Analytics, New York, NY) was used to perform demographic, clinical, and cognitive data analysis. The Q-Q plot and the Kolmogorov-Smirnov test were used to check normal distribution assumption of the variables. The chi-square test, the two-sample t-test, the Kruskal-Wallis test, and the Mann-Whitney test were performed for pairwise comparisons of the variables. All the statistically significant thresholds were set at 0.05.

Statistical analysis of cortical thickness was performed using FreeSurfer software. A general linear model was used to explore the differences of cortical thickness between the MSA subgroups and the HC group. The cluster-level statistical threshold was set at p < 0.05 and false discovery rate (FDR) method for multiple comparisons was used with age, gender, education, and disease duration as covariates of no interest. If no results were identified through this correction, a less strict threshold of p < 0.01, Monte Carlo corrected, was performed. The whole-brain GM volumes obtained with SPM software were compared among MSA subgroups and the HC group, and post hoc analysis was performed to explore between-group GM volume differences with the total intracranial volume, age, gender, and education added as covariates. Multiple comparisons were performed using the family wise error (FWE) correction, and the cluster-level statistical threshold was set at p < 0.05. Considering that our data do not conform to a normal distribution, Spearman correlation analysis (SPSS 20.0) and the regression models (SPM12) were performed to investigate the correlation of cortical thickness measures and GM volumes with the clinical and cognitive variables. A significance threshold of p < 0.05 was set.

Results

Demographic, Clinical, and Cognitive Findings

Forty-five MSA patients (MSA-NCI, 25; MSA-MCI, 20) and 29 HCs were enroled. There were no significant differences in age, sex, or level of education between groups. Both patient groups showed significantly lower MoCA scores compared with the HC group, while MSA-MCI patients showed significantly lower MoCA scores and higher UMSARSII scores compared with MSA-NCI patients, as expected. There were no significant differences between MSA-MCI and MSA-NCI patients in terms of UMSARSIII scores and duration of illness (Table 1).

TABLE 1

Demographic, clinical, and cognitive data of MSA and HC.

Demographic variable	MSA-NCI (n = 25)	MSA-MCI (n = 20)	HC (n = 29)	Z/χ2	p-value
Age (years)	62.52 ± 6.90	64.15 ± 9.22	61.17 ± 6.77	0.672	0.715
Sex (F:M)	12: 13	14: 6	20: 9	3.225	0.199
Education (years)	11.32 ± 3.18	11.70 ± 2.97	12.52 ± 3.10	2.040	0.361
Disease duration (years)	4.44 ± 2.10	3.80 ± 2.19	–	–1.254	0.210
MoCA	26.80 ± 1.22	20.10 ± 2.71	27.17 ± 1.23	46.454	0.000*
UMSARSII	7.24 ± 5.16	15.60 ± 2.76	–	4.592	0.000*
UMSARSIII	34.00 ± 13.59	38.90 ± 17.48	–	1.006	0.314

Cortical Thickness

Compared with the HC group, MSA-MCI patients showed significant cortical thinning in the frontal and temporal cortex of both hemispheres, especially in the bilateral superior frontal gyrus, bilateral superior temporal gyrus, bilateral medial orbito-frontal cortex, and supramarginal gyrus. Other areas of significant cortical thinning were found in the bilateral inferior parietal lobule, right inferior temporal gyrus and superior parietal lobule, left insular cortex and isthmus of cingulate, left paracentral gyrus, and left inferior frontal gyrus. Moreover, cortical thickness of the right middle temporal gyrus, left inferior and superior temporal gyrus, left paracentral lobule, left fusiform gyrus, left supramarginal gyrus, and pars triangularis were decreased in the MSA-NCI group compared with the HC group (p < 0.05 FDR). Compared to MSA-NCI patients, MSA-MCI patients showed cortical thinning in the left middle and inferior temporal gyrus (p < 0.01 Monte Carlo corrected) (Figure 1).

FIGURE 1

Alterations of cortical thickness: (A,B) false discovery rates correction was used (p < 0.05); (C) left hemisphere after Monte Carlo correction (p < 0.01). Warm colors represent areas of cortical thinning. Color bar represents t-values.

Gray Matter Volumetry

Regarding GM differences, MSA-MCI patients showed significantly reduced GM in the bilateral cerebellum, bilateral temporal area, right parahippocampal gyrus, left cingulate, and left putamen compared with the HC group. Relative to the HC group, MSA-NCI patients showed significantly decreased GM in the bilateral cerebellum, right fusiform gyrus extending to parahippocampal gyrus areas, and left lingual gyrus extending to fusiform gyrus areas. Compared with the MSA-NCI groups, MSA-MCI patients lost GM volume in the left middle temporal gyrus extending to superior temporal gyrus (p < 0.05 FWE) (Table 2 and Figure 2).

TABLE 2

Brain regions with significant GM volume reduction between MSA subgroups and HC.

Index brain region	Cluster size	Peak MNI coordinates			T-values	Z-score	P-value
	(Voxels)	X	Y	Z
HC vs. MSA-MCI
Temporal_Inf_L	871	–34.5	–3	–42	7.67	6.06	0.000*
Fusiform_R	1,544	36	–19.5	–31.5	8.08	6.27	0.000*
Amygdala_R	715	18	−1.5	–13.5	6.22	5.23	0.000*
Temporal_Mid_R	48	60	–27	–12	5.77	5.29	0.002
Putamen_L	457	–19.5	10.5	–12	6.40	5.34	0.000*
Cingulum_Mid_L	31	–4.5	–18	43.5	5.79	4.96	0.011
Temporal_Mid_L	59	–52.5	–55.5	12	6.32	4.94	0.002
HC vs. MSA-NCI
Fusiform_R	913	37.5	–21	–30	4.53	4.12	0.015
Lingual_L	1,894	–18	–61.5	–12	4.73	4.27	0.002
MSA-NCI vs. MSA-MCI
Temporal_Mid_L	1,237	–58.5	–25.5	–6	5.39	4.63	0.001

FIGURE 2

Alterations of gray matter volumes. Comparison between MSA-MCI patients and MSA-NCI patients. Family-wise error correction was used (p < 0.05). Warm colors represent areas of GM atrophy.

Correlations Between Cognitive Deficits and MRI Findings

In MSA-MCI patients, UMSARSII scores showed significant negative correlation with the extent of cortical thinning in the left precentral gyrus. Significant positive correlations between GM volume and MoCA scores were observed in the right superior and middle frontal gyrus. However, cortical thickness in the left middle temporal gyrus showed significant positive correlation with MoCA scores and negative correlation with duration of disease. No correlations were found with other clinical and cognitive deficits (Figure 3).

FIGURE 3

Scatter plots of cortical thickness and MoCA scores/duration of disease in left MTG.

Discussion

To explore the contribution of whole-brain morphology alterations on CI in MSA-MCI patients, based on the results of FreeSurfer and VBM, we investigated the relationship between cortical and subcortical structures and clinical measurements. Compared to the HC group, MSA-MCI patients showed widespread structural alterations in the fronto-parieto-temporal lobule and the limbic system. In the direct comparison between MSA-MCI and MSA-NCI, we found that MSA-MCI patients showed extensive GM atrophy in left superior and middle temporal gyrus and cortical thinning in left middle and inferior temporal gyrus. We also observed that cortical thinning of the left middle temporal gyrus was associated with cognitive decline and disease duration in MSA-MCI patients, which was in line with our hypotheses.

Several previous studies of a large sample of patients have confirmed the presence of cognitive impairment in MSA patients (Koga et al., 2017). Our results suggested that the alterations in cortical and subcortical structures in the left temporal region were a major cause of cognitive decline in MSA-MCI patients. Similar results have been reported in previous cross-sectional (Donix et al., 2018) and longitudinal (Whitwell et al., 2008) studies of patients with MCI. These studies demonstrated that the presence of significant temporal atrophy in MCI patients can be used to predict progression to dementia. However, our findings contradicted some previous studies of MSA patients (Chang et al., 2009; Fiorenzato et al., 2017; Koga et al., 2017; Caso et al., 2019), which suggested that morphological alterations in frontal lobule played a key role in cognitive impairment in MSA patients. Koga et al. (2017) and Caso et al. (2019) found that cognitive declines emanated from impaired frontal lobule in MSA. Fiorenzato et al. (2017) divided MSA patients into two groups, normal cognitive function and cognitive impairment, and they found that MSA patients with cognitive impairment showed greater frontal atrophy, illustrating that the dysfunction of the frontal-striatal connections might lead to cognitive impairment in MSA patients. Armstrong et al. (2005) found significant hypoperfusion in the frontal lobes of MSA patients, and hypoperfusion in the dorsolateral prefrontal cortex was associated with the severity of cognitive impairment. Both results were confirmed by anatomic and postmortem pathology studies, suggesting that the loss of neurons, astrocytic proliferation, and cytoplasmic inclusion bodies in the fronto-temporal regions might explain the cognitive decline in some MSA patients (Barcelos et al., 2018). Compared with these studies, the patients we enroled in our study were confined to the range of MCI, which might be a reason for the inconsistency between the results of our study and previous studies.

The temporal lobule plays a key role in cognition. Previous functional MRI studies suggested that the left temporal regions were involved in many cognitive processes, including semantic aspects of comprehension (superior temporal gyrus) (Friederici et al., 2003), language processing (superior and middle temporal gyrus) (Fan et al., 2017), semantic memory processing (middle temporal gyrus) (Kiehl et al., 2004; Wei et al., 2012; Fan et al., 2017), delayed memory (inferior temporal gyrus) (Kuroki et al., 2006), and visual processing (inferior temporal gyrus) (Kiernan, 2012; Ju et al., 2020). In addition, several recent studies confirmed that MSA-MCI patients had significant language decline, visual function decline, and memory impairment (Caso et al., 2019; Wang et al., 2019). Therefore, morphological changes in the left temporal region might be involved in cognitive impairment in MSA-MCI patients by affecting language, vision, and memory function. Our conjecture was confirmed by functional MRI studies. Zheng et al. (2019) found that the functional connection between cerebellum and left superior temporal gyrus in MSA patients was damaged, resulting in incomplete default mode network structure and multifunctional cognitive impairment. Ren et al. (2018) found that middle temporal gyrus involvement in the cerebellum and temporal cortical circuit functional connection damage caused the memory decline of MSA patients. Our findings were consistent with a previous study (Caso et al., 2019) showing that, for MSA patients, the involvement of not only the frontal but also the temporal regions was associated with cognitive impairment, as detected by structural imaging. These studies all emphasized the critical role of the temporal lobule in cognitive decline in MSA patients. Our findings, supported by Zhang et al. (2018), were consistent with one of the hypotheses regarding the underlying mechanism of cognitive impairment in MSA patients. Since we detected morphological changes in both VBM and FreeSurfer, we believed that the damage to the cerebral cortex and subcortical structure together led to cognitive impairment in MSA patients. Additionally, we also found that the temporal cortical thickness was significantly correlated with disease duration. Thus, the cortical thinning in the temporal region might be a predictor of increased risk for future dementia in MSA patients (Teipel et al., 2010).

Compared with the HC group, MSA-MCI patients also showed more widespread cortical thinning in the frontal lobule, however correlation results showed that the cortical thinning in the frontal lobule had no significant correlation with MoCA scores (r = 0.41; p = 0.07). Meanwhile no alterations were found in the frontal lobule in the direct comparison between MSA-MCI and MSA-NCI patients. Previous studies (Ozawa et al., 2004; Koga et al., 2017; Caso et al., 2019) have shown significant frontal lobe changes in MSA patients with cognitive impairment. Compared to these studies, the patients in our study were roughly in the mid-stage of disease (mean duration: MSA-MCI, 4.44 years; MSA-NCI, 3.80 years) and the cognitive impairment was relatively mild, so the contradictory results might be due to the disparate course of disease and different cognitive states. Compared with the HC group, we also found more widespread structural alterations in the basal ganglia and limbic system. Not surprisingly, pathologic study demonstrated that severe neuronal cytoplasmic inclusions in the limbic regions such as the hippocampus, the parahippocampal gyrus, the thalamus cortex, and the orbito-frontal cortex were associated with cognitive impairment in MSA patients (Wang et al., 2019).

Of note, the structural alterations in our MSA-MCI patients were mainly in the left rather than right hemisphere, and these results were observed in previous studies (Karas et al., 2003; Yang et al., 2019; Zhou et al., 2020). One possible explanation for this finding was that in our patient group, 33 had right-onset disease and 12 had left-onset disease. Previous reports suggested that ipsilateral brain structural alterations in MSA patients were related to contralateral clinical symptoms, showing significant asymmetry of clinical symptoms (Hwang et al., 2015; Yang et al., 2019). Some studies also suggested that a “time lag” may occur in the development of structural damage in the right hemisphere (Karas et al., 2003; Oishi et al., 2018). However, these conclusions were preliminary and need to be confirmed by more targeted study designs.

The present study has the following limitations. First, our sample size of patients with this rare condition is relatively small. Therefore, some of the differences we found in the regions between MSA-MCI and MSA-NCI did not exist after multiple comparison correction. Second, our study is a preliminary study on the cognitive impairment and morphological alterations of MSA-MCI patients. We did not examine the different effects of the brain on domain-specific cognitive changes. Future studies should focus on the relationship between various cognitive domains and brain structural alterations. Finally, we noticed that autonomic dysfunction might have an impact on the cognitive assessment in our study, so a rough assessment of autonomic function was achieved using UMSARSIII scales. However, more comprehensive and systematic quantitative tools, such as the Scopa-Aut questionnaire, would be helpful for future research.

In conclusion, the present study suggests that cognitive decline due to temporal region alterations might be characteristic of MSA-MCI patients. Since the underlying mechanism and brain alterations of MSA-MCI patients are largely unknown, the results of our study may be helpful to improve the knowledge of health care professionals on the important role of the temporal lobe in the progression of this disease, and this finding may be helpful to the future study of MSA. Future studies need to conduct multiparametric MRI studies on sufficient sample of patients to better understand the brain alterations in MSA patients.

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics Statement

The studies involving human participants were reviewed and approved by The First Affiliated Hospital of China Medical University. The patients/participants provided their written informed consent to participate in this study.

Author Contributions

CC, QW, and GF contributed to the study conception and design. HYu revised important intellectual content. CC and QW were analyzed and interpreted the data. CC was drafted the manuscript. GF was critically revised the manuscript and involved in study design, data interpretation, manuscript revision, and final approval of the version to be submitted. HYa, MG, YL, and HH performed data acquisition. All authors contributed to the article and approved the submitted version.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.