Literature DB >> 33806448

Expression of Interferons Lambda 3 and 4 Induces Identical Response in Human Liver Cell Lines Depending Exclusively on Canonical Signaling.

Mariia Lunova1, Jan Kubovciak2, Barbora Smolková3, Mariia Uzhytchak3, Kyra Michalova4, Alexandr Dejneka3, Pavel Strnad5, Oleg Lunov3, Milan Jirsa1,4.   

Abstract

Lambda interferons mediate antiviral immunity by inducing interferon-stimulated genes (ISGs) in epithelial tissues. A common variant rs368234815TT/∆G creating functional gene from an <span class="Gene">IFNL4 pseudogene is associated with the expression of major ISGs in the liver but impaired clearance of hepatitis C. To explain this, we compared Halo-tagged and non-tagged IFNL3 and IFNL4 signaling in liver-derived cell lines. Transfection with non-tagged IFNL3, non-tagged IFNL4 and Halo-tagged IFNL4 led to a similar degree of JAK-STAT activation and ISG induction; however, the response to transfection with Halo-tagged IFNL3 was lower and delayed. Transfection with non-tagged IFNL3 or IFNL4 induced no transcriptome change in the cells lacking either IL10R2 or IFNLR1 receptor subunits. Cytosolic overexpression of signal peptide-lacking IFNL3 or IFNL4 in wild type cells did not interfere with JAK-STAT signaling triggered by interferons in the medium. Finally, expression profile changes induced by transfection with non-tagged IFNL3 and IFNL4 were highly similar. These data do not support the hypothesis about IFNL4-specific non-canonical signaling and point out that functional studies conducted with tagged interferons should be interpreted with caution.

Entities:  

Keywords:  IFNLR1; IL10R2; interferon stimulated genes; knockout; transcriptome

Mesh：

Substances：

Year:  2021        PMID： 33806448      PMCID： PMC7961969          DOI： 10.3390/ijms22052560

Source DB:  PubMed          Journal:  Int J Mol Sci        ISSN： 1422-0067            Impact factor:   5.923


  40 in total

1.  featureCounts: an efficient general purpose program for assigning sequence reads to genomic features.

Authors:  Yang Liao; Gordon K Smyth; Wei Shi
Journal:  Bioinformatics       Date:  2013-11-13       Impact factor: 6.937

2.  Loss of function of the new interferon IFN-λ4 may confer protection from hepatitis C.

Authors:  David Booth; Jacob George
Journal:  Nat Genet       Date:  2013-02       Impact factor: 38.330

3.  Hepatic ISG expression is associated with genetic variation in interleukin 28B and the outcome of IFN therapy for chronic hepatitis C.

Authors:  Masao Honda; Akito Sakai; Tatsuya Yamashita; Yasunari Nakamoto; Eishiro Mizukoshi; Yoshio Sakai; Taro Yamashita; Mikiko Nakamura; Takayoshi Shirasaki; Katsuhisa Horimoto; Yasuhito Tanaka; Katsushi Tokunaga; Masashi Mizokami; Shuichi Kaneko
Journal:  Gastroenterology       Date:  2010-04-29       Impact factor: 22.682

Review 4.  Molecular and cellular mechanisms of liver fibrosis and its regression.

Authors:  Tatiana Kisseleva; David Brenner
Journal:  Nat Rev Gastroenterol Hepatol       Date:  2020-10-30       Impact factor: 46.802

Review 5.  IFN-λ4: the paradoxical new member of the interferon lambda family.

Authors:  Thomas R O'Brien; Ludmila Prokunina-Olsson; Raymond P Donnelly
Journal:  J Interferon Cytokine Res       Date:  2014-04-30       Impact factor: 2.607

6.  Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance.

Authors:  Dongliang Ge; Jacques Fellay; Alexander J Thompson; Jason S Simon; Kevin V Shianna; Thomas J Urban; Erin L Heinzen; Ping Qiu; Arthur H Bertelsen; Andrew J Muir; Mark Sulkowski; John G McHutchison; David B Goldstein
Journal:  Nature       Date:  2009-08-16       Impact factor: 49.962

Review 7.  Crystal structures of fusion proteins with large-affinity tags.

Authors:  Douglas R Smyth; Marek K Mrozkiewicz; William J McGrath; Pawel Listwan; Bostjan Kobe
Journal:  Protein Sci       Date:  2003-07       Impact factor: 6.725

8.  Transcriptome analysis reveals a classical interferon signature induced by IFNλ4 in human primary cells.

Authors:  C Lauber; G Vieyres; E Terczyńska-Dyla; R Dijkman; H H Gad; H Akhtar; R Geffers; F W R Vondran; V Thiel; L Kaderali; T Pietschmann; R Hartmann
Journal:  Genes Immun       Date:  2015-06-11       Impact factor: 2.676

Review 9.  Lambda interferons come to light: dual function cytokines mediating antiviral immunity and damage control.

Authors:  Evangelos Andreakos; Ivan Zanoni; Ioanna E Galani
Journal:  Curr Opin Immunol       Date:  2018-11-03       Impact factor: 7.486

10.  Ensembl 2019.

Authors:  Fiona Cunningham; Premanand Achuthan; Wasiu Akanni; James Allen; M Ridwan Amode; Irina M Armean; Ruth Bennett; Jyothish Bhai; Konstantinos Billis; Sanjay Boddu; Carla Cummins; Claire Davidson; Kamalkumar Jayantilal Dodiya; Astrid Gall; Carlos García Girón; Laurent Gil; Tiago Grego; Leanne Haggerty; Erin Haskell; Thibaut Hourlier; Osagie G Izuogu; Sophie H Janacek; Thomas Juettemann; Mike Kay; Matthew R Laird; Ilias Lavidas; Zhicheng Liu; Jane E Loveland; José C Marugán; Thomas Maurel; Aoife C McMahon; Benjamin Moore; Joannella Morales; Jonathan M Mudge; Michael Nuhn; Denye Ogeh; Anne Parker; Andrew Parton; Mateus Patricio; Ahamed Imran Abdul Salam; Bianca M Schmitt; Helen Schuilenburg; Dan Sheppard; Helen Sparrow; Eloise Stapleton; Marek Szuba; Kieron Taylor; Glen Threadgold; Anja Thormann; Alessandro Vullo; Brandon Walts; Andrea Winterbottom; Amonida Zadissa; Marc Chakiachvili; Adam Frankish; Sarah E Hunt; Myrto Kostadima; Nick Langridge; Fergal J Martin; Matthieu Muffato; Emily Perry; Magali Ruffier; Daniel M Staines; Stephen J Trevanion; Bronwen L Aken; Andrew D Yates; Daniel R Zerbino; Paul Flicek
Journal:  Nucleic Acids Res       Date:  2019-01-08       Impact factor: 16.971
View more
  4 in total

1.  High cysteine diet reduces insulin resistance in SHR-CRP rats.

Authors:  J Krijt; J Sokolová; J Šilhavý; P Mlejnek; J Kubovčiak; F Liška; H Malínská; M Hüttl; I Marková; M Křížková; M H Stipanuk; T Křížek; T Ditroi; P Nagy; V Kožich; M Pravenec
Journal:  Physiol Res       Date:  2021-09-10       Impact factor: 1.881

2.  Sodium Accumulation and Blood Capillary Rarefaction in the Skin Predispose Spontaneously Hypertensive Rats to Salt Sensitive Hypertension.

Authors:  Jan Šilhavý; Petr Mlejnek; Miroslava Šimáková; František Liška; Jan Kubovčiak; Eva Sticová; Michal Pravenec
Journal:  Biomedicines       Date:  2022-02-04

3.  Pathophysiology of Chronic Liver Disease Development.

Authors:  Malin Fromme; Pavel Strnad
Journal:  Int J Mol Sci       Date:  2022-03-21       Impact factor: 5.923

4.  Distinct molecular phenotypes involving several human diseases are induced by IFN-λ3 and IFN-λ4 in monocyte-derived macrophages.

Authors:  Manjarika De; Anand Bhushan; William S Grubbe; Subhajit Roy; Juan L Mendoza; Sreedhar Chinnaswamy
Journal:  Genes Immun       Date:  2022-02-03       Impact factor: 4.248
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.