| Literature DB >> 33795876 |
Lusheng Gu1,2,3,4,5, Yuanyuan Li1,2,5, Shuwen Zhang1,2,4,6, Maoge Zhou1,2,4, Yanhong Xue1,2,4, Weixing Li1,2,3,5, Tao Xu7,8,9,10,11, Wei Ji12,13,14,15,16.
Abstract
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.Entities:
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Year: 2021 PMID： 33795876 DOI： 10.1038/s41592-021-01099-2
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547