Compact and adjustable compensator for AOD spatial and temporal dispersion using off-the-shelf components.

Random access multiphoton microscopy using two orthogonal acousto-optic deflectors (AODs) allows sampling only particular regions of interest within a plane, greatly speeding up the sampling rate. AODs introduce spatial and temporal dispersions, which distort the point spread function and decrease the peak intensity of the pulse. Both of these effects can be compensated for with a single dispersive element placed a distance before the AODs. An additional acousto-optic modulator, a custom cut prism, and a standard prism used with additional cylindrical optics have been demonstrated. All of these introduce additional cost or complexity and require an extended path length to achieve the needed negative group delay dispersion (GDD). By introducing a telescope between a transmission grating and the AODs, we correct for spatial and temporal dispersions in a compact design using only off-the-shelf components, and we show that the GDD can be tuned by translation of the telescope without adjustment of any other elements.