Literature DB >> 33786461

CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos.

Caroline Hoppe1, Hilary L Ashe1.   

Abstract

CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila. For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).
© 2021 The Author(s).

Entities:  

Keywords:  CRISPR; Genetics; Model organisms; Molecular biology

Mesh：

Substances：

Year:  2021        PMID： 33786461      PMCID： PMC7988240          DOI： 10.1016/j.xpro.2021.100380

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  40 in total

1.  Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.

Authors:  Zhongsheng Yu; Mengda Ren; Zhanxiang Wang; Bo Zhang; Yikang S Rong; Renjie Jiao; Guanjun Gao
Journal:  Genetics       Date:  2013-07-05       Impact factor: 4.562

Review 2.  A Growing Toolbox to Image Gene Expression in Single Cells: Sensitive Approaches for Demanding Challenges.

Authors:  Xavier Pichon; Mounia Lagha; Florian Mueller; Edouard Bertrand
Journal:  Mol Cell       Date:  2018-08-02       Impact factor: 17.970

3.  Visualization of Transvection in Living Drosophila Embryos.

Authors:  Bomyi Lim; Tyler Heist; Michael Levine; Takashi Fukaya
Journal:  Mol Cell       Date:  2018-03-29       Impact factor: 17.970

4.  MS2 coat proteins bound to yeast mRNAs block 5' to 3' degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system.

Authors:  Jennifer F Garcia; Roy Parker
Journal:  RNA       Date:  2015-06-19       Impact factor: 4.942

5.  Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease.

Authors:  James E Dahlman; Omar O Abudayyeh; Julia Joung; Jonathan S Gootenberg; Feng Zhang; Silvana Konermann
Journal:  Nat Biotechnol       Date:  2015-11       Impact factor: 54.908

6.  Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.

Authors:  Fillip Port; Hui-Min Chen; Tzumin Lee; Simon L Bullock
Journal:  Proc Natl Acad Sci U S A       Date:  2014-07-07       Impact factor: 11.205

7.  Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

Authors:  Scott J Gratz; Alexander M Cummings; Jennifer N Nguyen; Danielle C Hamm; Laura K Donohue; Melissa M Harrison; Jill Wildonger; Kate M O'Connor-Giles
Journal:  Genetics       Date:  2013-05-24       Impact factor: 4.562

Review 8.  The CRISPR tool kit for genome editing and beyond.

Authors:  Mazhar Adli
Journal:  Nat Commun       Date:  2018-05-15       Impact factor: 14.919

9.  An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms.

Authors:  Oguz Kanca; Jonathan Zirin; Jorge Garcia-Marques; Shannon Marie Knight; Donghui Yang-Zhou; Gabriel Amador; Hyunglok Chung; Zhongyuan Zuo; Liwen Ma; Yuchun He; Wen-Wen Lin; Ying Fang; Ming Ge; Shinya Yamamoto; Karen L Schulze; Yanhui Hu; Allan C Spradling; Stephanie E Mohr; Norbert Perrimon; Hugo J Bellen
Journal:  Elife       Date:  2019-11-01       Impact factor: 8.140

10.  Modulation of the Promoter Activation Rate Dictates the Transcriptional Response to Graded BMP Signaling Levels in the Drosophila Embryo.

Authors:  Caroline Hoppe; Jonathan R Bowles; Thomas G Minchington; Catherine Sutcliffe; Priyanka Upadhyai; Magnus Rattray; Hilary L Ashe
Journal:  Dev Cell       Date:  2020-08-05       Impact factor: 12.270
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  4 in total

1.  Modelling the structure of Short Gastrulation and generation of a toolkit for studying its function in Drosophila.

Authors:  Sophie L Frampton; Catherine Sutcliffe; Clair Baldock; Hilary L Ashe
Journal:  Biol Open       Date:  2022-06-10       Impact factor: 2.643

Review 2.  Expanding Gene-Editing Potential in Crop Improvement with Pangenomes.

Authors:  Cassandria G Tay Fernandez; Benjamin J Nestor; Monica F Danilevicz; Jacob I Marsh; Jakob Petereit; Philipp E Bayer; Jacqueline Batley; David Edwards
Journal:  Int J Mol Sci       Date:  2022-02-18       Impact factor: 5.923

3.  One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster.

Authors:  Joyce J S Yu; Jean-Paul Vincent; Ian J McGough
Journal:  STAR Protoc       Date:  2021-05-25

4.  Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo.

Authors:  Daisy J Vinter; Caroline Hoppe; Hilary L Ashe
Journal:  STAR Protoc       Date:  2021-09-13
  4 in total

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