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Literature DB >> 23833182

Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.

Zhongsheng Yu¹, Mengda Ren, Zhanxiang Wang, Bo Zhang, Yikang S Rong, Renjie Jiao, Guanjun Gao.

Abstract

We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

Entities: Gene Species

Keywords: CRISPR/Cas9; Drosophila; NHEJ; gRNA; genome modifications

Mesh：

Year: 2013 PMID： 23833182 PMCID： PMC3761309 DOI： 10.1534/genetics.113.153825

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

13 in total

1. Gene targeting by homologous recombination in Drosophila.

Authors: Y S Rong; K G Golic
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2. Ends-out, or replacement, gene targeting in Drosophila.

Authors: Wei J Gong; Kent G Golic
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5. Generation of gene-modified mice via Cas9/RNA-mediated gene targeting.

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7. Multiplex genome engineering using CRISPR/Cas systems.

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8. Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos.

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9. Efficient genome editing in zebrafish using a CRISPR-Cas system.

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10. Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

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4. RNA-guided nucleases: a new era for engineering the genomes of model and nonmodel organisms.

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5. Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9.

Authors: Xingjie Ren; Jin Sun; Benjamin E Housden; Yanhui Hu; Charles Roesel; Shuailiang Lin; Lu-Ping Liu; Zhihao Yang; Decai Mao; Lingzhu Sun; Qujie Wu; Jun-Yuan Ji; Jianzhong Xi; Stephanie E Mohr; Jiang Xu; Norbert Perrimon; Jian-Quan Ni
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