Mazhar Salim Al-Zoubi1, Osama Otoum2, Mohammed Alsmadi3, Riyadh Muhaidat2, Ahmed Albdour3, Ziyad Mohaidat4, Manal Issam Abu Alarjah1, Raed M Al-Zoubi5, Khalid M Al-Batayneh2. 1. Yarmouk University, Faculty of Medicine, Department of Basic Medical Sciences, Irbid, Jordan. 2. Yarmouk University, Faculty of Science, Department of Biological Sciences, Irbid, Jordan. 3. King Hussein Medical Centre, Royal Medical Services, Amman, Jordan. 4. Jordan University of Science and Technology, Faculty of Medicine, Irbid, Jordan. 5. Jordan University of Science & Technology, Department of Chemistry, Irbid, Jordan.
Abstract
BACKGROUND: Intervertebral disc degeneration (IDD) is a musculoskeletal disorder and one of the major causes of low back pain leading to the disability with high economic repercussions worldwide. This study applied the candidategene approach to investigate the potential association of selected polymorphisms with IDD development in a Jordanian population. METHODS: MRI-diagnosed IDD patients (N=155) and asymptomatic individuals as a control group (N=55). Whole blood samples for four variants in three genes (rs1800587 of IL-1α, rs1143634 of IL-1β and rs2228570 and rs731236 of VDR) were genotyped by PCR-RFLP. RESULTS: There was no significant association between the studied polymorphisms or their allelic frequency and the occurrence of IDD. However, the cohort presented a significant reverse association between rs1143634 C > T of the IL-1β gene and the occurrence of IDD (p<0.0001). In addition, BMI showed a significant association with the IDD in the study population (p<0.005). The current study was conceptualized based on the candidate-gene approach to investigate the role of inflammatory and metabolic genes, IL and VDR, respectively, in the occurrence of IDD. CONCLUSIONS: While the data presented in this study showed that polymorphisms in these genes were not associated with IDD of the cohort investigated, elevated BMI, as a measure of obesity, is strongly associated with IDD. Investigating potential roles of other structural genes, such as col-IX and aggrecan (ACAN), in IDD and considering a GWAS to elucidate a genomically global look at the basis of IDD development would be of considerable impact on our understanding of IDD. 2021 Zoubi Mazhar Salim Al, Osama Otoum, Mohammed Alsmadi, Riyadh Muhaidat, Ahmed Albdour, Ziyad Mohaidat, Abu Alarjah Manal Issam, Raed M. Al-Zoubi, Khalid M. Al-Batayneh, published by CEON/CEES.
BACKGROUND: Intervertebral disc degeneration (IDD) is a musculoskeletal disorder and one of the major causes of low back pain leading to the disability with high economic repercussions worldwide. This study applied the candidategene approach to investigate the potential association of selected polymorphisms with IDD development in a Jordanian population. METHODS: MRI-diagnosed IDD patients (N=155) and asymptomatic individuals as a control group (N=55). Whole blood samples for four variants in three genes (rs1800587 of IL-1α, rs1143634 of IL-1β and rs2228570 and rs731236 of VDR) were genotyped by PCR-RFLP. RESULTS: There was no significant association between the studied polymorphisms or their allelic frequency and the occurrence of IDD. However, the cohort presented a significant reverse association between rs1143634 C > T of the IL-1β gene and the occurrence of IDD (p<0.0001). In addition, BMI showed a significant association with the IDD in the study population (p<0.005). The current study was conceptualized based on the candidate-gene approach to investigate the role of inflammatory and metabolic genes, IL and VDR, respectively, in the occurrence of IDD. CONCLUSIONS: While the data presented in this study showed that polymorphisms in these genes were not associated with IDD of the cohort investigated, elevated BMI, as a measure of obesity, is strongly associated with IDD. Investigating potential roles of other structural genes, such as col-IX and aggrecan (ACAN), in IDD and considering a GWAS to elucidate a genomically global look at the basis of IDD development would be of considerable impact on our understanding of IDD. 2021 Zoubi Mazhar Salim Al, Osama Otoum, Mohammed Alsmadi, Riyadh Muhaidat, Ahmed Albdour, Ziyad Mohaidat, Abu Alarjah Manal Issam, Raed M. Al-Zoubi, Khalid M. Al-Batayneh, published by CEON/CEES.
Authors: Kenneth M C Cheung; Danny Chan; Jaro Karppinen; Yiquin Chen; Jeffrey J T Jim; Shea-Ping Yip; Jurg Ott; Kelvin K Wong; Pak Sham; Keith D K Luk; Kathryn S E Cheah; John C Y Leong; You-Qiang Song Journal: Spine (Phila Pa 1976) Date: 2006-05-01 Impact factor: 3.468
Authors: Anthi Kelempisioti; Pasi J Eskola; Annaleena Okuloff; Ulla Karjalainen; Jani Takatalo; Iita Daavittila; Jaakko Niinimäki; Roberto B Sequeiros; Osmo Tervonen; Svetlana Solovieva; Patrick Y P Kao; You-Qiang Song; Kenneth M C Cheung; Danny Chan; Leena Ala-Kokko; Marjo-Riitta Järvelin; Jaro Karppinen; Minna Männikkö Journal: BMC Med Genet Date: 2011-11-22 Impact factor: 2.103