Genomic insights into biocontrol potential of edible seaweed-associated Bacillus velezensis MTCC 10456 from Gulf of Mannar.

Bacillus velezensis MTCC 10456, is a marine mesophilic heterotrophic bacterium, isolated from edible red seaweed, Laurenciae papillosa, with a potential for plant growth promotion and biocontrol activity. We report the genome sequence analysis of strain MTCC 10456, which has a genome size of 4.19 Mb with an average G + C content of 45.9%, 4077 coding sequences and 94 RNAs. Comparative genome analysis of MTCC 10456 with 76 other land plant-based strains with complete information on the source of their isolation, was carried out. This study provided evidence that multiple gene clusters that contributed to the seaweed colonization, growth promotion, immunity and hyperosmotic stress tolerance are conserved in different B. velezensis strains. A unique methyltransferase gene that can cause global alterations in DNA methylation patterns affecting gene expression and regulation of transcription in MTCC 10456 genome was identified. The genome provides insights into multiple gene clusters encoding antagonistic metabolites, such as non-ribosomal peptide synthetases (NRPSs) and polyketide synthetases (PKSs), unidentified metabolites and genes for antimicrobial resistance. Overall, findings from this study indicate the application of B. velezensis MTCC 10456 in the aquaculture industry as a biocontrol agent and may also contribute in understanding the ecological adaptations of plant-associated B. velezensis strains in different habitats.